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+ | <h1 class="box-heading"><i>Bacillus subtilis</i></h1> | ||
+ | <p id="B-subtilis"><i>B. subtilis</i> is one of the best-studied gram-positive microorganism, and a model bacterium for studying bacterial differentiation (e.g. endospore formation) and phenotypic heterogeneity.<a target="_blank" href ="https://www.ncbi.nlm.nih.gov/pubmed/19054118">[1]</a><a target="_blank" href ="https://www.ncbi.nlm.nih.gov/pubmed/20030732">[2]</a> Its ability to become naturally competent makes <i>B. subtilis</i> an organism which is easily accessible for genetic manipulation.<a target="_blank" href ="https://www.ncbi.nlm.nih.gov/pubmed/21278288">[3]</a> The GRAS (generally recognized as safe) status and the secretory capacity made <i>B. subtilis</i> a preferred host of choice for big scale production of secreted proteins, such as lipases, proteases and amylases, highlighting the industrial relevance of this bacterium.<a target="_blank" href ="https://www.ncbi.nlm.nih.gov/pubmed/16997527">[4]</a> <a target="_blank" href ="https://www.ncbi.nlm.nih.gov/pubmed/1368322">[5]</a></p> | ||
+ | <p> | ||
+ | In addition, the iGEM Team LMU-Munich 2012 has constructed the <i>Bacillus</i> BioBrickBox, which contains several well evaluated integrative vectors and other parts for the use in <i>B. subtilis</i>, thus providing a powerful toolbox to engineer <i>B. subtilis</i>.<a target="_blank" href ="https://www.ncbi.nlm.nih.gov/pubmed/24295448">[6]</a></p> | ||
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+ | <h1 class="box-heading">References</h1> | ||
+ | <table class="source"> | ||
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+ | <col width="95%"> | ||
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+ | <td><a target="_blank" href ="https://www.ncbi.nlm.nih.gov/pubmed/19054118">[1]</a></td> | ||
+ | <td>Lopez, D., Vlamakis, H. & Kolter, R. (2009) Generation of multiple cell types in <i>Bacillus subtilis</i>: from soil bacterium to super-secreting cell factory. <i>FEMS Microbiol. Rev.</i>, 33, 152–163.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a target="_blank" href ="https://www.ncbi.nlm.nih.gov/pubmed/20030732">[2]</a></td> | ||
+ | <td>Lopez, D. & Kolter, R. (2010) Extracellular signals that define distinct and coexisting cell fates in <i>Bacillus subtilis</i>. <i> FEMS Microbiol. Rev.</i> 34, 134–149</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a target="_blank" href ="https://www.ncbi.nlm.nih.gov/pubmed/21278288">[3]</a></td> | ||
+ | <td>Kaufenstein, M., van der Laan, M. & Graumann, P. L. (2011) The three-layered DNA uptake machinery at the cell pole in competent <i>Bacillus subtilis</i> cells is a stable complex. <i>J. Bacteriol.</i> 193, 1633–1642.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a target="_blank" href ="https://www.ncbi.nlm.nih.gov/pubmed/16997527">[4]</a></td> | ||
+ | <td>Fu L. L., Xu Z. R., Li W. F., Shuai J. B., Lu P. and Hu C. X. (2006) Protein secretion pathways in <i>Bacillus subtilis</i>: implication for optimization of heterologous protein secretion. <i>Biotechnology advances 25</i>, 1 (1-12).</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a target="_blank" href ="https://www.ncbi.nlm.nih.gov/pubmed/1368322">[5]</a></td> | ||
+ | <td>Harwood, C. R. (1992) <i>Bacillus subtilis</i> and its relatives: molecular biological and industrial workhorses. <i>Trends Biotechnol.</i> 10, 247–256</td> | ||
+ | <tr> | ||
+ | <td><a target="_blank" href ="https://www.ncbi.nlm.nih.gov/pubmed/24295448">[6]</a></td> | ||
+ | <td>Radeck, J., Kraft, K., Bartels, J., Cikovic, T., Dürr, F., Emenegger, J., Kelterborn, S., Sauer, C., Fritz, G., Gebhard, S., and Mascher, T. (2013) The <i>Bacillus</i> BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with <i>Bacillus subtilis</i>.<i> J Biol Eng 7</i>, 29.</td> | ||
+ | </tr> | ||
+ | </tr> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
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Revision as of 22:01, 1 November 2017