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<p>Protein secretion is mainly orchestrated by Signal Peptides (SP). However, secretion efficiency is not determined by the SP but the combination of a SP with a specific protein. This necessitates establishing screening procedures to evaluate all possible combinations. We developed such an approach, the Signal Peptide Toolbox, and succeeded in identifying the most potent SP-protein combinations for three different proteins. The Signal Peptide Toolbox enables an organism-independent, straightforward approach to enhance protein secretion levels.</p></figcaption> | <p>Protein secretion is mainly orchestrated by Signal Peptides (SP). However, secretion efficiency is not determined by the SP but the combination of a SP with a specific protein. This necessitates establishing screening procedures to evaluate all possible combinations. We developed such an approach, the Signal Peptide Toolbox, and succeeded in identifying the most potent SP-protein combinations for three different proteins. The Signal Peptide Toolbox enables an organism-independent, straightforward approach to enhance protein secretion levels.</p></figcaption> | ||
</figure></a> | </figure></a> | ||
+ | |||
<a href="https://2017.igem.org/Team:TU_Dresden/Composite_Part"> | <a href="https://2017.igem.org/Team:TU_Dresden/Composite_Part"> | ||
− | <figure style="width: | + | <figure style="width: 89%;" class="makeresponsive linkpicture"><img src="https://static.igem.org/mediawiki/2017/2/29/T--TU_Dresden--bacilli.png"> |
<figcaption><h2>Evaluation Vector</h2> | <figcaption><h2>Evaluation Vector</h2> | ||
<p>Peptidosomes offer a perfect platform for enhanced protein overproduction by the means of efficient protein secretion and purification due to physical separation of bacteria and the final product. In order to take full advantage of <i>B. subtilis</i>' native protein secretion potential, it is necessary to evaluate its secretion orchastrating signal peptides. Therefore, we developed the Evaluation Vector. This powerful genetic tool containing a multiple cloning site to easily build all combinatorial constructs of a protein with a set of signal peptides.</p></figcaption> | <p>Peptidosomes offer a perfect platform for enhanced protein overproduction by the means of efficient protein secretion and purification due to physical separation of bacteria and the final product. In order to take full advantage of <i>B. subtilis</i>' native protein secretion potential, it is necessary to evaluate its secretion orchastrating signal peptides. Therefore, we developed the Evaluation Vector. This powerful genetic tool containing a multiple cloning site to easily build all combinatorial constructs of a protein with a set of signal peptides.</p></figcaption> |
Revision as of 01:15, 2 November 2017