Difference between revisions of "Team:East Chapel Hill/Contribution"
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<h1>Contribution</h1> | <h1>Contribution</h1> | ||
<p style="font-size:18px;"> | <p style="font-size:18px;"> | ||
| − | Part: BBa_K2290000 </p> | + | Part: <a href="http://parts.igem.org/Part:BBa_K2290000">BBa_KK2990000</a> </p> |
<p style="font-size:18px;"> <b>Plasmid Backbone:</b> pSB1A3 </p> | <p style="font-size:18px;"> <b>Plasmid Backbone:</b> pSB1A3 </p> | ||
<p style="font-size:18px;"> | <p style="font-size:18px;"> | ||
| − | Below is a list of the basic parts that we used in our part <b | + | Below is a list of the basic parts that we used in our part <b> BBa_KK2990000</b> </p> |
<p style="font-size:18px;"> <b> BBa_J23100 </b> – a constitutively active promoter from the Anderson collection. </p> | <p style="font-size:18px;"> <b> BBa_J23100 </b> – a constitutively active promoter from the Anderson collection. </p> | ||
<p style="font-size:18px;"> <b> BBa_K2290008 </b> – The <i>B. cereus</i> riboswitch with an engineered terminator that results in less read through/background. (This part is highly similar to BBa_K911003, but the BBa_K911003 sequence includes both the Lys promoter and the riboswitch). </p> | <p style="font-size:18px;"> <b> BBa_K2290008 </b> – The <i>B. cereus</i> riboswitch with an engineered terminator that results in less read through/background. (This part is highly similar to BBa_K911003, but the BBa_K911003 sequence includes both the Lys promoter and the riboswitch). </p> | ||
Revision as of 01:37, 2 November 2017
Contribution
Part: BBa_KK2990000
Plasmid Backbone: pSB1A3
Below is a list of the basic parts that we used in our part BBa_KK2990000
BBa_J23100 – a constitutively active promoter from the Anderson collection.
BBa_K2290008 – The B. cereus riboswitch with an engineered terminator that results in less read through/background. (This part is highly similar to BBa_K911003, but the BBa_K911003 sequence includes both the Lys promoter and the riboswitch).
BBa_K2290004 – This is a sequence for a strong RBS that doesn't appear to be in the iGEM repository.
BBa_K2290005 – This is an E. coli optimized chloramphenicol resistance gene that we had synthesized.
BBa_B0010 – This is the T1 terminator from E. coli rrnB, reportedly a strong terminator
We characterized and improved upon part BBa_K911003 . First, BBa_K911003 listed as a basic part in the iGEM repository is actually a composite part that consists of a promoter and the B. cereus fluoride riboswitch. We more accurately annotated the B. cereus fluoride riboswitch alone as BBa_K2290008. We then characterized the responsiveness of the the B. cereus riboswitch and found that the switch works best at 100uM fluoride. To our knowledge part BBa_K911003 was not characterized in iGEM. We also used part BBa_J23100 from the Anderson promoter collection to regulate the expression of our operon and the BBa_B0010 terminator to efficiently terminate transcription . Given, that our composite part works these other two parts are functioning as intended in our operon.
