Difference between revisions of "Team:Paris Bettencourt/Results"

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<h1>Optogenetic</h1>
 
<h1>Optogenetic</h1>
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<div class=text2><div class=text2left><h4>Membrane photosensor</h4><ul><li></li></li></div>
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<div class=text2><div class=text2left><h4>Membrane photosensor</h4><ul><li> PHA production under two light AND-gate.</li><li> Characterisation of slow growth in multi-plasmid system. <li> Chacterisation of AND-gate using transmembrane photoreceptors.</li></ul></li></li></div>
 
       <div class=text2right><h4>Optics</h4><ul><li> Results confirm the feasibility of achieving a high targeting resolution at significant length within the gel medium.</li></ul></div></div>
 
       <div class=text2right><h4>Optics</h4><ul><li> Results confirm the feasibility of achieving a high targeting resolution at significant length within the gel medium.</li></ul></div></div>
 
<h1>Biomaterial</h1>
 
<h1>Biomaterial</h1>

Revision as of 02:13, 2 November 2017

RESULTS

Optogenetic

Membrane photosensor

  • PHA production under two light AND-gate.
  • Characterisation of slow growth in multi-plasmid system.
  • Chacterisation of AND-gate using transmembrane photoreceptors.

Optics

  • Results confirm the feasibility of achieving a high targeting resolution at significant length within the gel medium.

Biomaterial

Calcium Carbonate

  • Coral acid-rich proteins(CARP1-CARP4) were implemented into the expression vector and transformed into Escherichia coli BL21.
  • Right size proteins were expressed and checked for calcium carbonate precipitation ability.

Polysilicate

  • Characterized a biobrick from iGEM Pasteur

PHA

  • Improved the characterization of part BBa_K1149051 using flow cytometry to better quantify P3HB production in E. coli.

RNA organelle

  • RNA organelle was shown to decrease background expression of GFP, giving evidence to our model.
  • The RNA organelle was long-living, as it was observed to irreversible and stable in culture 20h after induction.

    Logic Gate & Photo-Caging

    • Successful testing in cell-free system with Dronpa.
    • Found evidence that Dronpa "sticks" the repressors to DNA.

    Human Practice

    Centre for Research and Interdisciplinarity (CRI)
    Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
    Paris Descartes University
    24, rue du Faubourg Saint Jacques
    75014 Paris, France
    bettencourt.igem2017@gmail.com