Difference between revisions of "Team:William and Mary/Part Collection"
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| − | <div style = 'padding-left: | + | <div style = 'padding-left: 14%; padding-bottom: 10px;font-size: 25px' ><b>Modular Protein Degradation System for Speed Control</b></div> |
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Our part collection provides future iGEM teams with the basic toolkit required to achieve modular, predictive speed control over their genetic circuits. We provide six distinct protein degradation tags (pdt) with distinct strengths and hence distinct effects on the speed of a tagged protein’s expression, in a ready-to-clone format for immediate use. We also provide constitutive and inducible reporter constructs tagged with each of the six tags. In total, this part collection represents the first experimental confirmation of a foundational mathematical model, as well as an accessible system that will allow future iGEM teams to access modular, predictive control over the temporal dynamics of their circuits by swapping parts at the genetic sequence level. We hope that our pdt system will, in addition to enabling this first foray into circuit speed control, serve as a foundation to drive continued innovations in controlling the dynamical properties of circuits. | Our part collection provides future iGEM teams with the basic toolkit required to achieve modular, predictive speed control over their genetic circuits. We provide six distinct protein degradation tags (pdt) with distinct strengths and hence distinct effects on the speed of a tagged protein’s expression, in a ready-to-clone format for immediate use. We also provide constitutive and inducible reporter constructs tagged with each of the six tags. In total, this part collection represents the first experimental confirmation of a foundational mathematical model, as well as an accessible system that will allow future iGEM teams to access modular, predictive control over the temporal dynamics of their circuits by swapping parts at the genetic sequence level. We hope that our pdt system will, in addition to enabling this first foray into circuit speed control, serve as a foundation to drive continued innovations in controlling the dynamical properties of circuits. | ||
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For more information on these, and the rest of our composite parts please see our <a href = "https://2017.igem.org/Team:William_and_Mary/Parts" style='text-decoration: underline;'>parts</a> page. We'd also like to ask that Judges consider these parts as fufillment of silver medal criteria I. For more information please see our <a href='https://2017.igem.org/Team:William_and_Mary/For_Judges' style='text-decoration: underline;'>for judges</a> page. | For more information on these, and the rest of our composite parts please see our <a href = "https://2017.igem.org/Team:William_and_Mary/Parts" style='text-decoration: underline;'>parts</a> page. We'd also like to ask that Judges consider these parts as fufillment of silver medal criteria I. For more information please see our <a href='https://2017.igem.org/Team:William_and_Mary/For_Judges' style='text-decoration: underline;'>for judges</a> page. | ||
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| − | <div style = 'padding-left: | + | <div style = 'padding-left: 14%; padding-bottom: 10px;font-size: 25px' ><b>Part Collection</b></div> |
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Latest revision as of 03:44, 2 November 2017
BioBrick ID
Construct
K2333401
Cloning Ready pdt A with Double Terminator
K2333402
Cloning Ready pdt B with Double Terminator
K2333403
Cloning Ready pdt C with Double Terminator
K2333404
Cloning Ready pdt D with Double Terminator
K2333405
Cloning Ready pdt E with Double Terminator
K2333406
Cloning Ready pdt F with Double Terminator
K2333413
UNS J23100 mScarlet-I
K2333414
UNS J23100 mScarlet-I pdt A
K2333415
UNS J23100 mScarlet-I pdt B
K2333416
UNS J23100 mScarlet-I pdt C
K2333417
UNS J23100 mScarlet-I pdt D
K2333418
UNS J23100 mScarlet-I pdt E
K2333419
UNS J23100 mScarlet-I pdt F
K2333427
UNS pTet mScarlet-I
K2333428
UNS pTet mScarlet-I pdt A
K2333429
UNS pTet mScarlet-I pdt B
K2333430
UNS pTet mScarlet-I pdt C
K2333431
UNS pTet mScarlet-I pdt D
K2333432
UNS pTet mScarlet-I pdt E
K2333433
UNS pTet mScarlet-I pdt F
K2333434
UNS plac mf-Lon
K2333435
UNS pBad mf-Lon
