Difference between revisions of "Team:TU Dresden/Experiments"
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<div class="contentbox"> | <div class="contentbox"> | ||
| − | <h1 class="box-heading"> | + | <h1 class="box-heading"> Cloning </h1> |
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/dc/New_cloning.pdf">Cloning Procedure</a> | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/dc/New_cloning.pdf">Cloning Procedure</a> | ||
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/db/SOP_protocols_for_cloning_with_the_Evaluation_Vector_and_the_Signal_Peptide_Toolbox.pdf">SOP Protocols for Cloning with the Evaluation Vector and the Signal Peptide Toolbox</a> | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/db/SOP_protocols_for_cloning_with_the_Evaluation_Vector_and_the_Signal_Peptide_Toolbox.pdf">SOP Protocols for Cloning with the Evaluation Vector and the Signal Peptide Toolbox</a> | ||
| − | < | + | </div> |
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| − | < | + | <div class="contentbox"> |
| + | <h1 class="box-heading">Working with <i>Bacillus subtilis</i></h1> | ||
<a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/0/05/Dna_isolation_for_pcr.pdf">DNA Isolation (for PCR)</a> | <a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/0/05/Dna_isolation_for_pcr.pdf">DNA Isolation (for PCR)</a> | ||
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/5/5f/Bacillus_transformation.pdf">Transformation of <i>B. subtilis </i></a> | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/5/5f/Bacillus_transformation.pdf">Transformation of <i>B. subtilis </i></a> | ||
| − | < | + | </div> |
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| − | < | + | <div class="contentbox"> |
| + | <h1 class="box-heading">Media and Solutions </h1> | ||
<a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/6/62/Media_antibiotics.pdf">Media, Solutions & Antibiotics</a> | <a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/6/62/Media_antibiotics.pdf">Media, Solutions & Antibiotics</a> | ||
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/03/Mcse.pdf">MCSE Medium</a> | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/03/Mcse.pdf">MCSE Medium</a> | ||
| − | < | + | </div> |
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| − | < | + | <div class="contentbox"> |
| + | <h1 class="box-heading">Primer</h1> | ||
<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/0f/Primer_list.pdf">Primerlist General</a> | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/0f/Primer_list.pdf">Primerlist General</a> | ||
<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/b/b8/Primer_list_signal_peptides.pdf">Primerlist Signalpeptides</a> | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/b/b8/Primer_list_signal_peptides.pdf">Primerlist Signalpeptides</a> | ||
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| − | |||
| + | </div> | ||
| − | + | <div class="contentbox"> | |
| − | + | <h1 class="box-heading">Assays</h1> | |
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| − | < | + | |
<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/c/c8/Disc_diffusion.pdf">Disc Diffussion Assay</a> | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/c/c8/Disc_diffusion.pdf">Disc Diffussion Assay</a> | ||
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/3/3b/Secretion_assay.pdf">Fluorescence Assay</a> | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/3/3b/Secretion_assay.pdf">Fluorescence Assay</a> | ||
| + | </div> | ||
| − | < | + | <div class="contentbox"> |
| − | + | <h1 class="box-heading">Protein Work</h1> | |
| − | < | + | |
<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/d9/Proteins.pdf">His-Tag Purification with Agarose Beads</a> | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/d9/Proteins.pdf">His-Tag Purification with Agarose Beads</a> | ||
<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/f/fa/Laemmli_SDS-PAGE.pdf">Laemmli SDS-PAGE</a | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/f/fa/Laemmli_SDS-PAGE.pdf">Laemmli SDS-PAGE</a | ||
| + | </div> | ||
| − | < | + | <div class="contentbox"> |
| − | + | <h1 class="box-heading">Peptidosome Methods </h1> | |
| − | < | + | |
<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/8/87/Protocols_peptidosomes.pdf">Peptidosome Creation</a> | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/8/87/Protocols_peptidosomes.pdf">Peptidosome Creation</a> | ||
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/4/41/Wellscan.pdf">Plate Reader Wellscan Peptidosomes</a> | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/4/41/Wellscan.pdf">Plate Reader Wellscan Peptidosomes</a> | ||
| − | + | </div> | |
Revision as of 17:07, 13 December 2017
