Team:Heidelberg/Sandbox1025

Week .DS_Store
Optopace
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Software
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Modeling
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Week 34
Optopace
Weekly summary 14.-20.08.2017 CG ==== Opto PACE === Primer for Opto PACE arrived and cloning was started. SP Opto -- PCR amplification of SP fragments: -- For the generation of the Opto SP two PCR fragments are necessary, SP_Opto_BB and EL222. SP_Opto_BB In order to amplify the SP Opto phage backbone primer CG_011_fwd and SP_R were used together with SP_ATMTAL (plasmid ID:8) as template in a 50 µl Q5 PCR reaction. Three different reaction mixtures were tested:

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Week 35
Optopace
Weekly summary 21.-27.08.2017 CG ==== Opto PACE === SP Opto -- PCR amplification of SP fragments: --- The blue-light mediated transcription activator EL222 was amplified from a gblock (EL222_expression_cassette) using CG_012_fwd and CG_013_rev as primers in a 25 µl Phusion Flash reaction mix. The cycler conditions for the 678 bp fragment were as follows:

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Week Icon
Optopace
No entry for this subproject this week.
Software
No entry for this subproject this week.
Modeling
No entry for this subproject this week.

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