Team:Paris Bettencourt/Hardware setup
Light Signalling in Gels
OPTIC MODEL
Medusa aims at controlling bacteria immobilized in a gel thanks to two laser beams. When entering the gel, the laser signal is affected by scattering and absorption . To ensure that the laser kept its adequate properties within the gel, we developed a strategy associating simple light data fitting and modelling to characterize the behaviour of a light beam within a gel.
We included in our study Agar (the most ubiquitous microbiology gel), Gelrite (which is more transparent than agar) and Alginate (which can be solidified at room temperature by addition of calcium ions). We chose a common working concentration of 1% w/v and studied our with LB nutrient (which surprisingly shows better transmission at most visible wavelengths than the M9 media). refer to our guide to choose the best gel for your optic experiments
The aim of our study is to enable the prediction of the light intensity in all points (x,y,z) of a volume of gel exposed to a laser beam. We employ a simple modelling strategy to represent the absorbance and the diffraction from the laser beam. Once calibrated, our model allows us to predict the light intensity landscape within a gel from a standard cuvette absorbance reading . We compare the predicted landscape to real intensity measurements and demonstrate that our model can accurately predict the resolution of our laser by solidifying light-curing resin particles of the predicted size.
We included in our study Agar (the most ubiquitous microbiology gel), Gelrite (which is more transparent than agar) and Alginate (which can be solidified at room temperature by addition of calcium ions). We chose a common working concentration of 1% w/v and studied our with LB nutrient (which surprisingly shows better transmission at most visible wavelengths than the M9 media). refer to our guide to choose the best gel for your optic experiments
The aim of our study is to enable the prediction of the light intensity in all points (x,y,z) of a volume of gel exposed to a laser beam. We employ a simple modelling strategy to represent the absorbance and the diffraction from the laser beam. Once calibrated, our model allows us to predict the light intensity landscape within a gel from a standard cuvette absorbance reading . We compare the predicted landscape to real intensity measurements and demonstrate that our model can accurately predict the resolution of our laser by solidifying light-curing resin particles of the predicted size.
RNA is a light cost nucleotide material in the cell,
We aim to recreate RNA agglomerations as formed
in mammalian cells with triple repeat disorders,
which show liquid phase separation, forming a
organelle-like vesicle, where local concentrations of
enzymes can be created.
RNA is a light cost nucleotide material in the cell,
We aim to recreate RNA agglomerations as formed
in mammalian cells with triple repeat disorders,
which show liquid phase separation, forming a
organelle-like vesicle, where local concentrations of
enzymes can be created.
RNA is a light cost nucleotide material in the cell,
We aim to recreate RNA agglomerations as formed
in mammalian cells with triple repeat disorders,
which show liquid phase separation, forming a
organelle-like vesicle, where local concentrations of
enzymes can be created.
RNA is a light cost nucleotide material in the cell,
We aim to recreate RNA agglomerations as formed
in mammalian cells with triple repeat disorders,
which show liquid phase separation, forming a
organelle-like vesicle, where local concentrations of
enzymes can be created.
SECOND MODEL
your text
RNA is a light cost nucleotide material in the cell,
We aim to recreate RNA agglomerations as formed
in mammalian cells with triple repeat disorders,
which show liquid phase separation, forming a
organelle-like vesicle, where local concentrations of
enzymes can be created.
RNA is a light cost nucleotide material in the cell,
We aim to recreate RNA agglomerations as formed
in mammalian cells with triple repeat disorders,
which show liquid phase separation, forming a
organelle-like vesicle, where local concentrations of
enzymes can be created.
RNA is a light cost nucleotide material in the cell,
We aim to recreate RNA agglomerations as formed
in mammalian cells with triple repeat disorders,
which show liquid phase separation, forming a
organelle-like vesicle, where local concentrations of
enzymes can be created.
RNA is a light cost nucleotide material in the cell,
We aim to recreate RNA agglomerations as formed
in mammalian cells with triple repeat disorders,
which show liquid phase separation, forming a
organelle-like vesicle, where local concentrations of
enzymes can be created.
THIRD MODEL
your text
RNA is a light cost nucleotide material in the cell,
We aim to recreate RNA agglomerations as formed
in mammalian cells with triple repeat disorders,
which show liquid phase separation, forming a
organelle-like vesicle, where local concentrations of
enzymes can be created.
RNA is a light cost nucleotide material in the cell,
We aim to recreate RNA agglomerations as formed
in mammalian cells with triple repeat disorders,
which show liquid phase separation, forming a
organelle-like vesicle, where local concentrations of
enzymes can be created.
RNA is a light cost nucleotide material in the cell,
We aim to recreate RNA agglomerations as formed
in mammalian cells with triple repeat disorders,
which show liquid phase separation, forming a
organelle-like vesicle, where local concentrations of
enzymes can be created.
RNA is a light cost nucleotide material in the cell,
We aim to recreate RNA agglomerations as formed
in mammalian cells with triple repeat disorders,
which show liquid phase separation, forming a
organelle-like vesicle, where local concentrations of
enzymes can be created.
Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
bettencourt.igem2017@gmail.com
bettencourt.igem2017@gmail.com 
