Protocols
Here are the protocols we used. We are very proud that we did them all to achieve our goal for iGEM competition this year.
Protocol for Animal Experiment: https://static.igem.org/mediawiki/2017/6/6f/T--SCU-WestChina--Wiki-Notebook-Protocol-animal.pdf
Protocol for BCA: https://static.igem.org/mediawiki/2017/8/8d/T--SCU-WestChina--Wiki-Notebook-protocol-bca.pdf
Protocol for eGFP Purification: https://static.igem.org/mediawiki/2017/2/2c/T--SCU-WestChina--Wiki-Notebook-protocol-eGFP_Purification.pdf
Protocol for Enzymatic Digestion & Ligation: https://static.igem.org/mediawiki/2017/2/27/T--SCU-WestChina--Wiki-Notebook-protocol-enzyme.pdf
Protocol for Fast cloning: https://static.igem.org/mediawiki/2017/8/80/T--SCU-WestChina--Wiki-Notebook-protocol-fast_floning.pdf
Protocol for Gibson assembling: https://static.igem.org/mediawiki/2017/e/e8/T--SCU-WestChina--Wiki-Notebook-protocol-gibson.pdf
Protocol for Knock-out: https://static.igem.org/mediawiki/2017/7/78/T--SCU-WestChina--Wiki-Notebook-protocol-knock_out.pdf/p>
Protocol for PCR: https://static.igem.org/mediawiki/2017/4/43/T--SCU-WestChina--Wiki-Notebook-protocol-pcr.pdf
Protocol for Special Tests: https://static.igem.org/mediawiki/2017/a/a9/T--SCU-WestChina--Wiki-Notebook-protocol-special.pdf