Over the past three years, iGEM has advanced the frontiers of science with the biggest interlaboratory studies ever done in synthetic biology. The SCU-WestChina iGEM team is honored to participate in the Interlab study this year.
The aim of the Interlab study is to analyze the measurement data of fluorescence with a detailed protocol, but different lab equipment and environment. In this way, researchers are able to get an access to compare fluorescence data from laboratories all over the world, in a standardized way.
InterLab 2017 Plate Reader Protocol was followed. The device utilized in our Interlab experiments was BioTek Cytation™ 3.
For this study, following constructs are used:
•Negative Control: I20270
•Positive Control: R0040
•Test Device 1: J23101.BCD2.E0040.B0015
•Test Device 2: J23106.BCD2.E0040.B0015
•Test Device 3: J23117.BCD2.E0040.B0015
•Test Device 4: J23101+I13504
•Test Device 5: J23106+I13504
•Test Device 6: J23117+I13504
All devices and parts were obtained from the iGEM 2017 InterLab Distribution Kit. Devices were made with a pSB1C3 plasmid backbone and transformed into Escherichia coli DH5α.
Results from the Plate Reader indicated that the promoter of the Device 4 is strongest, followed by the promoter of the Device 2 and Device 1.
The lowest Intensity was detected in Device 3, Device 5 and Device 6.