Plasmid
1. First, choose your preferred backbone from the pull down menu. We provide three different origins of replication that differ in their copy number and therefore affect the selection stringency, your AP will have. Be aware that the origin of replication should be compatible to the origin of replication of all other plasmids you plan to use. In most of the cases it is best to combine your origin of replication with an ampicillin resistance, but we provide other antibiotic resistances for special applications as well.
2. Second, decide which RBS you need for control of geneIII translation. We offer six different RBS with different strengths. For further information look...
3. Next, decide, which reporter you would like to choose. A set of different fluorescent and luminescent reporters is available
Resistance & Ori
RBS + geneIII
RBS + Reporter
Activator & Promoter
4. When all standard parts are defined, the promoter and associated regulatory sequences must be set. Decide between the given options. Enter either an own sequence (as text or upload the file) or use one of the provided standard promoters that were already used by our team. Notice that the length of this part should not be below 200 bp. Ensure, that your sequence does not include a RBS.
