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@iGEM_Paris

Light Signalling in Gels

OPTIC MODEL

Introduction

Medusa aims at optically controlling bacteria immobilized in a gel by targetting them with two intersecting lasers. Two questions are important to address to achieve this goal:

How far can a laser go in a gel?

What resolution can the laser achieve?

We present in this page our study of the optical properties of commonly used culture gels. We show that it is possible to obtain a clean light signal even at gel depths greater than 10cm.

Gel Choice

The optical properties of a gel depends on the gel type, its concentration and the nutrient source. For our study we focused on Agar, Alginate and Gelrite (see Table 1)
It is best to use a low gel concentration to reduce absorbance. Conveniently some gels have a higher melting point than solidifying point. This hysteresis phenomenon means that the gel concentration can be lowered and still be kept at in a solid state at 37C by pre-cooling them (to 6-8C) prior to warm incubation. Alginate gelling is induced by calcium ions which crosslink gelling polymers at room temperature. To get a homogenous gel, calcium ions cannot be mixed with an Alginate solution but must diffuse from an underlying calcium source (1). Since the optical properties of Alginate depends on the amount of crosslinking, they ultimately depend on calcium diffusion which is a difficult parameter to control. It is important to note that keeping the same diffusion conditions (time, temperature, calcium source concentration etc.) is necessary to maintain identical alginate optical properties between experiments.

Table 1: Gel Included in this Study

	Alginate	Agar	Gelrite
Key advantage	Solidifies at room temperature by adding calcium ion	Most ubiquitous microbiology gel	Low absorbance
Recommended concentration	1.75-4%	1-1.5%	0.15-0.25%
Melting-Gelling Point	NA	0.5%:85-20C 1%:90-35C	0.5%:65-15 1%:100-35C
Key disadvantage	Properties depend on calcium ion diffusion which is hard to control	High absorbance	Melts less well than agar
Concentration studied	1% with LB	1% with LB	1% & 0.5% with LB

RNA is a light cost nucleotide material in the cell, We aim to recreate RNA agglomerations as formed in mammalian cells with triple repeat disorders, which show liquid phase separation, forming a organelle-like vesicle, where local concentrations of enzymes can be created.

RNA is a light cost nucleotide material in the cell, We aim to recreate RNA agglomerations as formed in mammalian cells with triple repeat disorders, which show liquid phase separation, forming a organelle-like vesicle, where local concentrations of enzymes can be created.

SECOND MODEL

your text

RNA is a light cost nucleotide material in the cell, We aim to recreate RNA agglomerations as formed in mammalian cells with triple repeat disorders, which show liquid phase separation, forming a organelle-like vesicle, where local concentrations of enzymes can be created.

RNA is a light cost nucleotide material in the cell, We aim to recreate RNA agglomerations as formed in mammalian cells with triple repeat disorders, which show liquid phase separation, forming a organelle-like vesicle, where local concentrations of enzymes can be created.

RNA is a light cost nucleotide material in the cell, We aim to recreate RNA agglomerations as formed in mammalian cells with triple repeat disorders, which show liquid phase separation, forming a organelle-like vesicle, where local concentrations of enzymes can be created.

RNA is a light cost nucleotide material in the cell, We aim to recreate RNA agglomerations as formed in mammalian cells with triple repeat disorders, which show liquid phase separation, forming a organelle-like vesicle, where local concentrations of enzymes can be created.

THIRD MODEL

your text

RNA is a light cost nucleotide material in the cell, We aim to recreate RNA agglomerations as formed in mammalian cells with triple repeat disorders, which show liquid phase separation, forming a organelle-like vesicle, where local concentrations of enzymes can be created.

RNA is a light cost nucleotide material in the cell, We aim to recreate RNA agglomerations as formed in mammalian cells with triple repeat disorders, which show liquid phase separation, forming a organelle-like vesicle, where local concentrations of enzymes can be created.

RNA is a light cost nucleotide material in the cell, We aim to recreate RNA agglomerations as formed in mammalian cells with triple repeat disorders, which show liquid phase separation, forming a organelle-like vesicle, where local concentrations of enzymes can be created.

RNA is a light cost nucleotide material in the cell, We aim to recreate RNA agglomerations as formed in mammalian cells with triple repeat disorders, which show liquid phase separation, forming a organelle-like vesicle, where local concentrations of enzymes can be created.