Team:ZJUT-China/Description
ZJUT-China
Description
- References and sources we used in our research.
Nowadays intracellular products take up a great share o the market, and with the increase of people’s need, they will create a growing market value. But the way to extra the target products from inside of the cells has always been a problem. Chemical disruption ways will mix in new impurities which need to be separated later, and physical ways may bring high temperature leading to unstable products so they need chillers. Considering of this problem, we came up with an idea that if cells could self-lyse under the control of some convenient conditions provided by environment, for example, the light because light serves as an excellent trigger to achieve precise control on synthetic systems as it can regulate in wavelength, timing, intensity and location. Based on this thought, the project showed in the video just came out. In particular, we focused on Escherichia coli in the project because it’s common used and easy to operate. The project may fit other bacteria in further study.
①Jeffrey J. Tabor,Anselm Levskaya,and Christopher A. Voigt. Multichromatic control of gene expression in Escherichia coli.J Mol Biol. 2011 January 14; 405(2): 315–324.
②Wu H, Wang Y, Wang Y, Cao X, Wu Y, Meng Z, Su Q, Wang Z, Yang S, Xu W, Liu S, Cheng P, Wu J, Khan MR, He L, Ma G.Quantitatively relating gene expression to light intensity via the serial connection of blue light sensor and CRISPRi.ACS Synth Biol. 2014 Dec 19;3(12):979-82. doi: 10.1021/sb500059x.
③Yoshihiro Ojima, Minh Hong Nguyen, Reiki Yajima, and Masahito Taya.Flocculation of Escherichia coli Cells in Association with Enhanced Production of Outer Membrane Vesicles. Appl Environ Microbiol. 2015 Sep 1;81(17):5900-6. doi: 10.1128/AEM.01011-15. Epub 2015 Jun 19.
④Laura Grande, Valeria Michelacci, Rosangela Tozzoli, Paola Ranieri, Antonella Maugliani, Alfredo Caprioli, and Stefano Morabito. Whole genome sequence comparison of vtx2-converting phages from Enteroaggregative Haemorrhagic Escherichia coli strains. BMC Genomics. 2014; 15(1): 574.Published online 2014 Jul 8. doi: 10.1186/1471-2164-15-574.
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References
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