Team:William and Mary/Part Collection

Proof of Concept

Our part collection provides future iGEM teams with the basic toolkit required to achieve modular, predictive speed control over their genetic circuits. We provide six distinct protein degradation tags (pdt) with distinct strengths and hence distinct effects on the speed of a tagged protein’s expression, in a ready-to-clone format for immediate use. We also provide constitutive and inducible reporter constructs tagged with each of the six tags. In total, this part collection represents the first experimental confirmation of a foundational mathematical model, as well as an accessible system that will allow future iGEM teams to access modular, predictive control over the temporal dynamics of their circuits by swapping parts at the genetic sequence level. We hope that our pdt system will, in addition to enabling this first foray into circuit speed control, serve as a foundation to drive continued innovations in controlling the dynamical properties of circuits.

Part	Description
K2333401	UNS pdt#3 double stop DT
K2333402	UNS pdt#3a double stop DT
K2333403	UNS pdt#3b double stop DT
K2333404	UNS pdt#3c double stop DT
K2333405	UNS pdt#3d double stop DT
K2333406	UNS pdt#3e double stop DT
K2333413	UNS J23100 mScarlet-I
K2333414	UNS J23100 mScarlet-I pdt A
K2333415	UNS J23100 mScarlet-I pdt B
K2333416	UNS J23100 mScarlet-I pdt C
K2333416	UNS J23100 mScarlet-I pdt D
K2333416	UNS J23100 mScarlet-I pdt E
K2333416	UNS J23100 mScarlet-I pdt F

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