Team:ZJUT-China/Results
ZJUT-China
Results
Experiments
- The functional gene lysin (BBa_K2277000) was tested using OD600 testing&dilution-spread method and works well.
- The functional gene bcsB (BBa_K2277110) has been tested and being abandoned.
- The reporter gene eGFP (BBa_K2277033) works well as reporter gene.
- The new FixK2 promoter (BBa_K2277233) has improved the efficiency of the existing part BBa_K1065303.
- The light-controlled part (BBa_K2277555) has been tested using eGFP as the reporter gene and works well.
- The whole light-controlled lysis system (BBa_K2277666) is constructed successfully and was tested using OD600 testing.
The whole system’s constitution is shown in the picture.
Cells will self-lyse in the absence of blue light.
Device
| Light Source | Light Intensity | Compositions | Parameters | Notes |
|---|---|---|---|---|
| When we were making the circuit boards using as the light source, function had a priority.According to the requirements of our cells culture, which is, a fixed wavelength of the blue light irradiation which could start our lysin gene.Therefore, in the process of experimental variables involved, we controlled the wavelength and strength of light source. Then we built bottom fixed wavelength light source in the completely dark black case. In order to control conveniently, we designed a multi paralleled DC circuit which was simple, stable and switchable. Battery powered LED lamps were chosen as the light source so that the device would be easy to carry out. | 34lux | Bottom: pvc cover | ф7.6cmcm*0.5cm | |
| Bottom: thick cardboard | Bottom: thick cardboard |
- Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
- Show data, but remember all measurement and characterization data must be on part pages in the Registry.
- Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.
Project Achievements
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- A list of linked bullet points of the successful results during your project
- A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.
Inspiration
See how other teams presented their results.
