Team:ZJUT-China/Description
ZJUT-China
Description
- Wu H, Wang Y, Wang Y, Cao X, Wu Y, Meng Z, Su Q, Wang Z, Yang S, Xu W, Liu S, Cheng P, Wu J, Khan MR, He L, Ma G., Quantitatively relating gene expression to light intensity via the serial connection of blue light sensor and CRISPRi. ACS Synth Biol. 2014, 3(12): 979-982
- Gardner L, Deiters A. Light-controlled synthetic gene circuits. Curr Opin Chem Biol. 2012, 16(3-4):292-299
- Camsund D, Lindblad P, Jaramillo A. Genetically engineered light sensors for control of bacterial gene expression. Biotechnol J. 2011, 6(7):826-836
- Ohlendorf R, Vidavski RR, Eldar A, Moffat K, Möglich A., From dusk till dawn: one-plasmid systems for light-regulated gene expression. J Mol Biol. 2012, 416(4): 534-542
- Sun Q, Kuty GF, Arockiasamy A, Xu M, Young R, Sacchettini JC., Regulation of a muralytic enzyme by dynamic membrane topology. Nat Struct Mol Biol. 2009 Nov;16(11):1192-1194
Conception
Nowadays intracellular products take up a great share o the market, and with the increase of people’s need, they will create a growing market value. But the way to extra the target products from inside of the cells has always been a problem. Chemical disruption ways will mix in new impurities which need to be separated later, and physical ways may bring high temperature leading to unstable products so they need chillers. Considering of this problem, we came up with an idea that if cells could self-lyse under the control of some convenient conditions provided by environment, for example, the light, because light serves as an excellent trigger to achieve precise control on synthetic systems as it can regulate in wavelength, timing, intensity and location. Based on this thought, the project showed in the video just came out. In particular, we focused on Escherichia coli in the project because it’s common used and easy to operate. The project may fit other bacteria in further study.
References and sources we used in our research.
Advice on writing your Project Description
We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
References
iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.
Inspiration
See how other teams have described and presented their projects:
