We went to Hangzhou Zhongmei Huadong Pharmaceutical Co., Ltd. introducing our project to their director of Macromolecular Laboratory and asking for the possibility that if our blue-light-controlled self-lysis system could fit their production in the middle of our experiments. But our system was consisted of two plasmids (one for light-controlled part and one for FixK2 promoter and lysin) at that time. Therefore the director told us only if we could solve the problem of multi-plasmids pressure in cells then could our project be used in fermentation industry. We had spent a long time connecting the light-controlled system and the functional gene so that they can be put in the same one plasmid. In some ways we solved the problem of multi-plasmids pressure by reducing one plasmid in the system. And we may work on making one kind of plasmid vector with our blue-light-controlled self-lysis system which can be directly used by fermentation factories for genetically engineered producing strains in the future.

Others

To make our human practice work more efficient! We held different activities involving safety, public education and social activities in different modus according to the group in different age.

Questionnaire

As our project is related to intracellular product, we did a survey among the public to know their attitude to synthetic technology as well as how much they know about iGEM.Here goes the result.The main content

Question 3: Have you ever heard about synthetic biology?

Analysis: only 1/5 of respondents have heard and know synthetic biology which is a main kind of technology used in the industry. The result implied that it’s necessary to spread the knowledge to the public.

Question 7: Have you ever used or heard about intracellular product?

Analysis: nowadays intracellular products take a share of the market, but they don’t arose enough attention. The result shows that only 20% of the respondents have ever used and cared about product.

Question 12: Among the following traditional method used in disrupting cells to extra the intracellular product, which method have you heard about ?

Analysis: cell disruption in the industry are traditionally under harsh condition. We are surprised to find that 25% of the respondents thought it was no unnecessary. We listed several common method in the questionnaire , hoping it would help a bit.

Question 14: If the engineering bacteria can be disrupted by the light what’s your attitude towards the technology ?

Analysis: a majority of the respondents hold a active attitudes to our idea, as it’s more gentle and more environment friendly, though they didn’t understand the technology thoroughly.

According to the result, we can get the conclusion that, public don’t acquire enough knowledge about synthetic biology as well as intracellular product, but they appeared the awareness of clean producing which is also our idea aims at. Therefore, the propaganda work and public education are both essential.

Best Integrated Human Practices Special Prize

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Inspiration

Here are a few examples of excellent Integrated Human Practices work:

Team:ZJUT-China/HP/Gold Integrated

ZJUT-China

Gold Medal and Integrated Human Practices

Gold Medal Criterion #1

Best Integrated Human Practices Special Prize

Inspiration