Difference between revisions of "Team:Grenoble-Alpes/Protocols"
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Revision as of 15:39, 29 August 2017
Protocols
Preparations
Experimentations
LB Broth
Materials
Materials
- Add 20g of LB Broth Base per 1L of distillated water
- Homogenize
- Autoclave at 121°C for 15 minutes
CLOSE
LB Agar
Materials
Materials
- Add 32g of LB Agar per 1L of distilled water
- Homogenize
- Autoclave at 121°C for 15 minutes
CLOSE
Chloramphenicol (Cam) 25ug/mL Stock
Materials
Materials
- Add 0,25g of Cam per 10mL of ethanol
- Homogenize by vortexing
- Filter with a 22mm membrane filter
- Conserve at -20°C in 1mL aliquot
CLOSE
Petri Dish Preparation
Materials
Materials
- Add of 20uL Cam in 20mL of liquid LB Agar
- Pour the solution in the plate
- Wait until the agar solidifies
- Conserve returned in 4°C
CLOSE
Glycerol 80%
Materials
Materials
- Harvest 80mL of glycerol 100%
- Add 20mL of sterile water
- Conserve at 4°C
CLOSE
CaCl2 100mM
Materials
Materials
- Dissolve 0,056g of CaCl2 in 5mL of sterile water
- Conserve at 4°C
CLOSE
MgCl2 100mM
Materials
Materials
- Dissolve 0,6105g of MgCl2 in 30mL of sterile water
- Conserve at 4°C
CLOSE
Sucrose 100mM
Materials
Materials
- Dissolve 0,171g of sucrose in 5mL of sterile water
- Conserve at 4°C
CLOSE
IPTG 4mM
Materials
Materials
- Add 10uL of Cam in 10mL of LB
- Homogenize
- Remove 90uL of solution and add 80uL of IPTG
- Conserve at -20°C
CLOSE
