Difference between revisions of "Team:Grenoble-Alpes/Protocols"

Line 95: Line 95:
 
         </header>
 
         </header>
  
−
<!- -------------- PROTOCOLS LOGO --------------- ->
+
<!- -------------- PROTOCOLS --------------- ->
  
 
       <h2 class="titre_categorie" id="prep" style="padding-bottom: 2%; background:#27ae60; margin:0%;"> Preparations </h2>
 
       <h2 class="titre_categorie" id="prep" style="padding-bottom: 2%; background:#27ae60; margin:0%;"> Preparations </h2>
Line 110: Line 110:
 
               <a href="#iptg" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 
               <a href="#iptg" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 
         </center></div>
 
         </center></div>
−
 
−
      <h2 class="titre_categorie" id="exp" style="padding-bottom: 2%; background:#27ae60; margin:0%;"> Experimentations </h2>
 
−
 
−
        <div style="width:100%; margin: 2% 0%;"><center>
 
−
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 
−
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 
−
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 
−
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 
−
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 
−
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 
−
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 
−
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 
−
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 
−
        </center></div>
 
−
 
−
<!- -------------- PROTOCOLS DETAILS --------------- ->
 
  
 
<div id="lb_broth">
 
<div id="lb_broth">
Line 271: Line 255:
 
   <a href="#prep" style="text-decoration: none;"><h2 class="cls">CLOSE</h2></a>
 
   <a href="#prep" style="text-decoration: none;"><h2 class="cls">CLOSE</h2></a>
 
</div>
 
</div>
 +
 +
      <h2 class="titre_categorie" id="exp" style="padding-bottom: 2%; background:#27ae60; margin:0%;"> Experimentations </h2>
 +
 +
        <div style="width:100%; margin: 2% 0%;"><center>
 +
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 +
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 +
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 +
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 +
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 +
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 +
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 +
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 +
              <a href="#" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 +
        </center></div>
 +
  
 
<!- -------------- RESULTS BANNER --------------- ->
 
<!- -------------- RESULTS BANNER --------------- ->

Revision as of 15:48, 29 August 2017

Lab

Protocols

Preparations

LB Broth

Materials
  • 20g LB Broth Base (powder)
  • 1L Distillated Water
    • Methods
    1. Add 20g of LB Broth Base per 1L of distillated water
    2. Homogenize
    3. Autoclave at 121°C for 15 minutes

    CLOSE

    LB Agar

    Materials
  • 32g LB Agar (powder)
  • 1L Distilled Water
    • Methods
    1. Add 32g of LB Agar per 1L of distilled water
    2. Homogenize
    3. Autoclave at 121°C for 15 minutes

    CLOSE

    Chloramphenicol (Cam) 25ug/mL Stock

    Materials
  • 0,25g Chloramphenicol (25mg/mL)
  • 10mL Ethanol
    • Methods
    1. Add 0,25g of Cam per 10mL of ethanol
    2. Homogenize by vortexing
    3. Filter with a 22mm membrane filter
    4. Conserve at -20°C in 1mL aliquot

    CLOSE

    Petri Dish Preparation

    Materials
  • 20mL LB Agar
  • 20uL Cam (25ug/mL)
    • Methods
    1. Add of 20uL Cam in 20mL of liquid LB Agar
    2. Pour the solution in the plate
    3. Wait until the agar solidifies
    4. Conserve returned in 4°C

    CLOSE

    Glycerol 80%

    Materials
  • 80mL Glycerol 100%
  • 20mL Sterile Water
    • Methods
    1. Harvest 80mL of glycerol 100%
    2. Add 20mL of sterile water
    3. Conserve at 4°C

    CLOSE

    CaCl2 100mM

    Materials
  • 0,0555g CaCl2 (111g/mol)
  • 5mL Sterile Water
    • Methods
    1. Dissolve 0,056g of CaCl2 in 5mL of sterile water
    2. Conserve at 4°C

    CLOSE

    MgCl2 100mM

    Materials
  • 0,610g MgCl2 (203,31g/mol)
  • 30mL Sterile Water
    • Methods
    1. Dissolve 0,6105g of MgCl2 in 30mL of sterile water
    2. Conserve at 4°C

    CLOSE

    Sucrose 100mM

    Materials
  • 0,171g Sucrose
  • 5mL Sterile Water
    • Methods
    1. Dissolve 0,171g of sucrose in 5mL of sterile water
    3. Conserve at 4°C

    CLOSE

    IPTG 4mM

    Materials
  • 80uL IPTG (500mM)
  • 10mL LB
  • 10µL Cam (25ug/mL)
    • Methods
    1. Add 10uL of Cam in 10mL of LB
    2. Homogenize
    3. Remove 90uL of solution and add 80uL of IPTG
    4. Conserve at -20°C

    CLOSE

    Experimentations

    Designed by iGEM Grenoble-Alpes 2017 team | Site Map