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<p style="font-size:1.6em;color:black;margin-bottom:5px"><b>Sensing Plasmid</b></p> | <p style="font-size:1.6em;color:black;margin-bottom:5px"><b>Sensing Plasmid</b></p> | ||
− | The second half of our project revolved around an assembly that sensed the production of L-DOPA. | + | <p>The second half of our project revolved around an assembly that sensed the production of L-DOPA. |
<br></br> | <br></br> | ||
Our sensing construct consisted of several ---. We constructed this assembly using golden gate, gibson, and traditional cloning techniques. | Our sensing construct consisted of several ---. We constructed this assembly using golden gate, gibson, and traditional cloning techniques. | ||
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<li>Venus fluorescent protein (BBa_---): | <li>Venus fluorescent protein (BBa_---): | ||
<li>rpoc terminator (BBa_K2458001): | <li>rpoc terminator (BBa_K2458001): | ||
− | + | </p> | |
</p> | </p> | ||
Revision as of 17:22, 1 November 2017
Design
Production Plasmid
Part of our project revolved around an assembly that produced L-DOPA (levadopa or L-3,4-dihydroxyphenylalanine) from glucose.
We constructed our production assembly by cloning a CP25/PA3-hpaBC-rpoc transcriptional unit into a vector containing a --- ori and spectinomycin resistance marker. Our construct was assembled using golden gate cloning techniques.
Diagram here.
Here is a brief overview of each main constituent of the hpaBC transcriptional unit:
Sensing Plasmid
The second half of our project revolved around an assembly that sensed the production of L-DOPA.
Our sensing construct consisted of several ---. We constructed this assembly using golden gate, gibson, and traditional cloning techniques.
Diagram here.
Here is a brief overview of the transcriptional units and their functions.
lacI Transcriptional Unit -
This page is different to the "Applied Design Award" page. Please see the Applied Design page for more information on how to compete for that award.
What should this page contain?
- Explanation of the engineering principles your team used in your design
- Discussion of the design iterations your team went through
- Experimental plan to test your designs
Inspiration