Difference between revisions of "Team:Austin UTexas LASA/Notebook"
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| − | <p> | + | <p>Week 1: Did PCR to get HpaB out of the HpaBC lab plasmid, sequence verified ppddc in BTK001 backbone, sequence verified pp2551 and HpaC, made glycerol stocks of sequence verified parts |
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| − | <h4 class="media-heading"> | + | <h4 class="media-heading">Oct 10 - Oct 14, 2016</h4> |
| − | <p> | + | <p>Week 2: Ordered HpaBC operon from IDT, grew up pEM7 [BTK156], did Gibson assembly of HpaBC in YTK001 entry vector and grew it up |
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| − | <h4 class="media-heading"> | + | <h4 class="media-heading">Oct 17 - Oct 21, 2016</h4> |
| − | <p> | + | <p>Week 3: Split parts into different teams, each team working on assembling and sequence verifying individual parts into entry vecotrs[ppddc, HpaBC pEM7, pp2551] |
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| + | <h4 class="media-heading">Oct 24 - Oct 28, 2016</h4> | ||
| + | <p>Week 4: Grew up and miniprepped HpaBC, and pp2551, but transformation of HpaBC did not work. | ||
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Revision as of 22:25, 1 November 2017
Notebook
Sept 1 - Sept 9, 2016
Week 1: Got aliquots of HpaBC from lab, grew up parts (Hpab, HpaC, pp2551) and digested them to check lengths.
Oct 3- Oct 7, 2016
Week 1: Did PCR to get HpaB out of the HpaBC lab plasmid, sequence verified ppddc in BTK001 backbone, sequence verified pp2551 and HpaC, made glycerol stocks of sequence verified parts
Oct 10 - Oct 14, 2016
Week 2: Ordered HpaBC operon from IDT, grew up pEM7 [BTK156], did Gibson assembly of HpaBC in YTK001 entry vector and grew it up
Oct 17 - Oct 21, 2016
Week 3: Split parts into different teams, each team working on assembling and sequence verifying individual parts into entry vecotrs[ppddc, HpaBC pEM7, pp2551]
Oct 24 - Oct 28, 2016
Week 4: Grew up and miniprepped HpaBC, and pp2551, but transformation of HpaBC did not work.
What should this page have?
- Chronological notes of what your team is doing.
- Brief descriptions of daily important events.
- Pictures of your progress.
- Mention who participated in what task.
Inspiration
You can see what others teams have done to organize their notes:
