Difference between revisions of "Team:CCU Taiwan/HP/Gold Integrated"

Line 222: Line 222:
 
   <section>
 
   <section>
  
<embed src="https://static.igem.org/mediawiki/2017/6/62/%E5%B0%8F%E6%9B%B86.0_5.pdf" width="110%" height="600px">
+
<h2>Gold and integrated</h2>
<div id="Fluorescein">
+
<h2>Fluorescein Fluorescence standard curve</h2>
+
  
</div>
+
<p>
 +
After we thought some foundation survey, we found that Carindex maybe would be needed in Taiwan. So we made a series of survey to observe the use willingness.<br/>
 +
We also thought some public communicate to know what are they need, and we seek to their opinion, helping we can improve our device.
 +
</p>
  
<div id="Fluorescein-Plate-reader">
+
<h2>Survey</h2>
<div class="aaa"></div>
+
<h3>Plate reader</h3>
+
  
    <p>
+
<p>1. Online product form survey and PMR survey: In order to know what our products can expand to the community, as well as the public for the use of our products will be willingness, and whether foreign regions are also used for our product necessary. We conducted online product form questionnaire survey and PMR. (Potential Market Research)<br/>
      microplate reader FLUOstar Omega</br>
+
We real took to ask on the streets, to understand the real needs of users, to expect to get the relevant data. We expect that our products can be more in line with the needs of users. <br/>
emission filter: 520 nm</br>
+
The survey results are as follows:<br/>
excitation filter: 485 nm
+
Total sample<br/>
    </p>
+
Taiwan: 187 persons<br/>
 
+
Foreign: 70 person
</div>
+
</p>
 
+
<div id="Fluorescein-Material">
+
<div class="aaa"></div>
+
<h3>Material</h3>
+
<p>
+
Fluorescein sodium salt</br>
+
1xPBS</br>
+
Tissue culture testplate (black with flat bottom)
+
</p>
+
 
+
</div>
+
  
<div id="Fluorescein-Method">
+
<img src="https://static.igem.org/mediawiki/2017/9/94/G1.jpg" style="display:block; margin:auto;"><br/>
<div class="aaa"></div>
+
<img src="https://static.igem.org/mediawiki/2017/8/8e/G2.jpg" style="display:block; margin:auto;"><br/>
                <h3>Method</h3>
+
<img src="https://static.igem.org/mediawiki/2017/7/7d/G3.jpg" style="display:block; margin:auto;"><br/>
+
<img src="https://static.igem.org/mediawiki/2017/6/65/G4.jpg" style="display:block; margin:auto;"><br/>
<ol><li>Prepare fluorescein stock solution</li></ol>
+
<h3>Foreign</h3>
 +
<img src="https://static.igem.org/mediawiki/2017/3/3d/G5.jpg" style="display:block; margin:auto;"><br/>
 +
<img src="https://static.igem.org/mediawiki/2017/8/89/G6.jpg" style="display:block; margin:auto;"><br/>
 +
<img src="https://static.igem.org/mediawiki/2017/c/cd/G7.jpg" style="display:block; margin:auto;"><br/>
 +
<img src="https://static.igem.org/mediawiki/2017/4/4d/G8.jpg" style="display:block; margin:auto;"><br/>
 +
<img src="https://static.igem.org/mediawiki/2017/5/51/G9.jpg" style="display:block; margin:auto;"><br/>
 +
<img src="https://static.igem.org/mediawiki/2017/5/5d/G10.jpg" style="display:block; margin:auto;"><br/>
 +
<h3>Summary</h3>
 
<p>
 
<p>
1. Spin down fluorescein stock tube to make sure pellet is at the bottom of tube.</br>  
+
From above information, we can see that the range of 7-12 years old children, school will have a school dentist to check. (Before we asked to the primary school staff, the sample of 36 parents, 93% agreed. And nine schools, all that willing use this product, and will have the intention to let the children with the check), so they think that don’t need to buy this for children.<br/>
2. Prepare 2x fluorescein stock solution (100 μM) by resuspending fluorescein in 1 mL of 1xPBS. </br>
+
16 years old will be own regular go to dentist.<br/>
3. Dilute the 2x fluorescein stock solution with 1xPBS to make a 1x fluorescein solution and resulting concentration of fluorescein  stock solution 50 μM </br></br>
+
19 years old are basically no need. (more factors)<br/>
 +
We found that approximately 73% people don’t want to use this product, because they feel only go to dentist enough, and the product is expensive.<br/>
 +
On the other hand, foreign people have more high willingness to use this product in home, because they would pay more money to go to dentist, compare this product price, the product is much cheaper.
 
</p>
 
</p>
<ol><li>Serial dilutions</li></ol>
 
<p>
 
1. Add 100 μl of PBS into wells A2, B2, C2, D2....A12, B12, C12, D12</br>
 
2. Add 200 μl of fluorescein 1x stock solution into A1, B1, C1, D1</br>
 
3. Transfer 100 μl of fluorescein stock solution from A1 into A2. </br>
 
4. Mix A2 by pipetting up and down 3x and transfer 100 μl into A3. </br>
 
5. Mix A3 by pipetting up and down 3x and transfer 100 μl into A4. </br>
 
6. Mix A4 by pipetting up and down 3x and transfer 100 μl into A5. </br>
 
7. Mix A5 by pipetting up and down 3x and transfer 100 μl into A6. </br>
 
8. Mix A6 by pipetting up and down 3x and transfer 100 μl into A7. </br>
 
9. Mix A7 by pipetting up and down 3x and transfer 100 μl into A8. </br>
 
10. Mix A8 by pipetting up and down 3x and transfer 100 μl into A9. </br>
 
11. Mix A9 by pipetting up and down 3x and transfer 100 μl into A10.</br>
 
12. Mix A10 by pipetting up and down 3x and transfer 100 μl into A11. </br>
 
13. Mix A11 by pipetting up and down 3x and transfer 100 μl into liquid waste.
 
  (Caution: Do not to continue serial dilution into column 12.)</br>
 
</p>
 
<ol><li>repeat serial dilute for Row B、D、E</strong></li></ol>
 
<ol><li>Measure fluorescence of all samples in all standard measurement modes in instrument Record the data in your notebook</strong></li></ol>
 
<ol><li>Import data into Excel (fluorescein standard curve tab ) Sheet_1 provided</li></ol>
 
 
<br/><br/>
 
  
</div>
+
<h2>Communicate with public</h2>
  
<div id="Fluorescein-Data-result">
+
<h3>Product pamphlet</h3>
<div class="aaa"></div>
+
<h3>Data result</h3>
+
<br/>
+
<img src="https://static.igem.org/mediawiki/2017/8/85/FFs_1.png" style="display:block; margin:auto;"><br/><br/>
+
<img src="https://static.igem.org/mediawiki/2017/a/a4/FFs_2.png" style="display:block; margin:auto;"><br/><br/>
+
<img src="https://static.igem.org/mediawiki/2017/5/5c/FFs_3.png" style="display:block; margin:auto;"><br/>
+
</div>
+
</section>
+
  
<section>
+
<p>
 +
In order to make the public more convenient and clear understanding of our products, we made a pamphlet for the research and development of products. In the pamphlet, we briefly introduced the use of the product, technology, security and other issues, and tell their in which we used genetically modified technology in the product. We had design a gene that is a kill switch in our gene design, so that when we not only contact to the public in communication can be more convenient to understand the safety of our products, but also help our products Promotion.
 +
</p>
  
 +
<embed src="https://static.igem.org/mediawiki/2017/6/62/%E5%B0%8F%E6%9B%B86.0_5.pdf" width="100%" height="600px">
  
<div id="OD600">
+
<h3>Public booth activity</h3>
<h2>OD600 Reference point</h2>
+
<p>
 +
In order to enable our products to actually reach the public, through communication with the public to improve our products, so that products can be more intimate social needs. We cooperate with kumquat farm from Taiwan Chiayi local famous tourist attractions. In the crowd booth, we put our analog product and product pamphlet on our booth, to the public who introduced a lot about our project, including health care teeth of acknowledge, as well as the function of our products and follow-up benefits. Many parents and children after our introduction, not only have a great interest in our products, but also like our exquisite analog device. In which we have received a lot of feedback from the general public, and we have been more understand to their facing problem of oral cavity. Which will help us to improve the product and make the product more in line with the needs of the user!
 +
</p>
  
</div>
+
<img src="https://static.igem.org/mediawiki/2017/a/a9/Eng1.jpg" style="display:block; margin:auto;"><br/>
 +
<img src="https://static.igem.org/mediawiki/2017/9/99/Eng2.jpg" style="display:block; margin:auto;"><br/>
  
 +
<h2>Improve our product</h2>
  
<div id="OD600-Plate-reader">
+
<h3>Visited to dentist</h3>
<div class="aaa"></div>
+
<p>
<h3>Plate reader</h3>
+
In order to be able to make our products more in line with the actual social needs of the situation, we visited a number of Taiwan Chiayi local dental clinic: 百利、沃爾、邱. In several visits, we understand the actual needs of the patient from the perspective of the dentist and the patient actual oral cavity.  We also have a better understanding in the common consumer habits of patients. The information is great help us to plan how to send the product to the public, and what kind of use can make the user the most convenient and greatest help.
 +
</p>
  
    <p>
+
<img src="https://static.igem.org/mediawiki/2017/8/8b/Eng9.jpg" width="400" height="550" style="display:block; margin:auto; float:left;">
Thermo Scientific™ Multiskan™ FC Filter-based Microplate Photometer</br>
+
<img src="https://static.igem.org/mediawiki/2017/b/ba/Eng10.jpg" width="350" height="550" style="display:block; margin:auto;"><br/>
Filter: 595 nm</br>
+
    </p>
+
  
</div>
+
<h3>Dentist collaboration</h3>
 +
<p>
 +
In order to enable our data to be accurate, we collaborate with a number of local dentists in Chiayi. Asking them to help the team define our product internal data. When we visited the dentist, we discussed the concept of our product with the dentist, and then we asked whether the dentist could set up the database for the team to build the product. The dentist was very enthusiastic to help us build the product database. We hope to be able to use the dentist's expertise to ensure the accuracy of the product. In which the standard for defining the oral tooth decay are the standards established by the ICDAS (International Caries Detection and Assessment System) organization. This standard is recommended by the students of the dentistry department.
 +
</p>
  
<div id="OD600-Material">
+
<img src="https://static.igem.org/mediawiki/2017/e/ef/Eng11.jpg" style="display:block; margin:auto;"><br/>
  <div class="aaa"></div>
+
<img src="https://static.igem.org/mediawiki/2017/6/62/Eng12.jpg"  width="700" style="display:block; margin:auto;"><br/>
<h3>Material</h3>
+
<p>
+
1 ml LUDOX</br>
+
mQH<sub>2</sub>O</br>
+
96 well cell culture plate (clear with flat-bottom)
+
</p>
+
  
</div>
+
<h3>Primary school collaboration</h3>
 +
<p>
 +
In the course of our contact with the public, we found that the vast majority of parents are willing to buy this product to the children at home, so we turn to visit primary school students in Chiayi, and ask whether there is a willingness to collaborate to use the device. The scope of the user extends to the children. In the interview process, we found that the school current will ask the dentist to school to examine the Taiwanese young children every three months, if the dentist found the children that the oral condition is not good, He will ask the children to see again dentist after child back to home. In this process, the dentist check children oral condition is often too long, causing many inconvenience. Therefore, the school said that if the device in the follow-up to improve the accuracy, then very willing to cooperate with us, hope that through our team of device instead of dentists for children to check the mouth, hoping to easily check the process.
 +
</p>
  
<div id="OD600-Method">
 
<div class="aaa"></div>
 
                <h3>Method</h3>
 
  
 +
<h2>Integrated</h2>
 +
<h3>Get advises and improve device</h3>
 
<p>
 
<p>
1. Add 100 μl LUDOX into wells A1, B1, C1, D1 (or 1 mL LUDOX into cuvette)</br>
+
After visiting many people, elementary school students and public booth, they are interested in our products and expect that we can continue to develop this product, hoping to improve the accurate so that the future for health care will be a very good product. As a result, we have made the following improvements to our products after we have given us the advice of the public.
2. Add 100 μl of H<sub>2</sub>O into wells A2, B2, C2, D2 (or 1 mL H<sub>2</sub>O into cuvette)</br>
+
3. Measure absorbance 600 nm of all samples in all standard measurement modes in instrument</br>
+
4. Record the data in excel and Import data into Excel ( OD600 reference point tab ) Sheet_1 provided</br>
+
 
</p>
 
</p>
 
</div>
 
  
<div id="OD600-Data-result">
+
<ol><li>To improve the appearance of the device</ol></li>
<div class="aaa"></div>
+
<p>
<h3>Data result</h3>
+
After we cooperate the local elementary school, they suggested that we can design the device into a lovely and can attract style of children that will increase the use of children willing!
<br/>
+
</p>
<img src="https://static.igem.org/mediawiki/2017/c/cd/Ee.jpeg" style="display:block; margin:auto;"><br/>
+
</div>
+
</section>
+
  
<section>
+
<ol><li>The installation of the slide was originally required to use three slides and was to be installed in three installments. Now change to install once</ol></li>
 +
<p>
 +
After we communicate with a lot of public, they feedback that the device is not easy to use. They hope that they can be installed like an ink clip of printer. Therefore, we are going to change the product design. The original product would be used to three slides, and each slide must be installed, the design is too troublesome, so we combined the three slide into one set, the user only install once when he will use it. That is to simplify the use process of the product.
 +
</p>
  
 +
<ol><li>Product appearance of the material from iron to acrylic</ol></li>
 +
<p>
 +
When we actual contact with the public and our product form, the vast majority of people said that the sale price of the product is too high. Therefore, we will change the appearance of the product material, from the original appearance of iron changed to acrylic material to reduce the cost of the product, so that product prices can be reduced.
 +
4. Adding an extra space for germinating consumptive material:After we discussed with the local primary school, the health center staff to provide some advices on our products. They are hoping that we can set a space that can placed next to the slide space in the device. The space will prepare to slide that is next one will using in device. When health care personnel are check to mouth of children, which can easy and quickly to take the slide, the design not only easy to manage those flow of slides, but also to avoid the children casually took the slide to cause accident in the inspection. Each class requires the use of three instruments, each of our instruments can be placed in the preparation of slides 8 to 9, a total of about 25 to meet the Taiwanese member of each class.
  
<div id="Cell">
+
</p>
<h2>Cell measure</h2>
+
  
</div>
 
  
  
<div id="Cell-Material">
 
  <div class="aaa"></div>
 
<h3>Material</h3>
 
<p>
 
Competent cells ( Escherichia coli strain DH5α)</br>
 
LB (Luria Bertani) media</br>
 
Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH - working stock 25 μg /mL)</br>
 
50 ml Falcon tube (or equivalent, preferably amber or covered in foil to block light)</br>
 
Incubator at 37°C</br>
 
1.5 ml eppendorf tubes for sample storage</br>
 
Ice bucket with ice</br>
 
Pipettes</br>
 
96 well plate(cell culture 96 well plate、tissue culture testplate)</br>
 
Devices (from InterLab Measurement Kit):</br>
 
1. Negative control(BBa_R0040)</br>
 
2. Positive control(J23151+B0032+E0040+B0010+B0012)</br>
 
3. Test Device 1: J23101+I13504</br>
 
4. Test Device 2: J23106+I13504</br>
 
5. Test Device 3: J23117+I13504</br>
 
6. Test Device 4: J23101+BCD2+E0040+B0015</br>
 
7. Test Device 5: J23106+BCD2+E0040+B0015</br>
 
8. Test Device 6: J23117+BCD2+E0040+B0015</br>
 
</p>
 
  
</div>
 
  
<div id="Cell-Method">
 
<div class="aaa"></div>
 
                <h3>Method</h3>
 
 
<p>
 
1. Day 1 : Resuspended each plasmid in plate 7 and transform into Escherichia coli DH5α.</br>
 
&nbsp;(Transformation protocol is from iGEM)</br>
 
2. Day 2 : Pick 2 colonies from each of plate and inoculate it on 5-10 mL LB medium +Chloramphenicol.Grow the cells overnight (16-18 hours) at 37°C and 170 rpm.</br>
 
3. Day 3 : Set instrument to read OD600 (as OD calibration setting)and measure OD600 of the overnight cultures</br>
 
4. Dilute the cultures to a target OD 600 of 0.02 in 12 ml LB medium + Chloramphenicol in 50 mL falcon tube (covered with foil to block light).</br>
 
5. Incubate the cultures at 37°C and 170 rpm.</br>
 
6. Take 1000 μL samples of the cultures at 0, 2, 4, and 6 hours of incubation and place samples on ice</br>
 
7. 4 replicates of 100 uL samples were taken from each culture at 0, 2, 4, and 6 hours of incubation and placed in a 96 well plate for OD and fluorescence measurements using the setup described above</br>
 
</p>
 
  
</div>
 
  
<div id="Cell-Data-result">
 
<div class="aaa"></div>
 
<h3>Data result</h3>
 
<br/>
 
<img src="https://static.igem.org/mediawiki/2017/f/f7/IL_cell1.jpg" style="display:block; margin:auto;"><br/><br/>
 
<img src="https://static.igem.org/mediawiki/2017/4/41/IL_cell2.jpg" style="display:block; margin:auto;"><br/>
 
</div>
 
 
</section>
 
</section>
  

Revision as of 17:46, 29 October 2017

No Sidebar - Helios by HTML5 UP

Gold and integrated

After we thought some foundation survey, we found that Carindex maybe would be needed in Taiwan. So we made a series of survey to observe the use willingness.
We also thought some public communicate to know what are they need, and we seek to their opinion, helping we can improve our device.

Survey

1. Online product form survey and PMR survey: In order to know what our products can expand to the community, as well as the public for the use of our products will be willingness, and whether foreign regions are also used for our product necessary. We conducted online product form questionnaire survey and PMR. (Potential Market Research)
We real took to ask on the streets, to understand the real needs of users, to expect to get the relevant data. We expect that our products can be more in line with the needs of users.
The survey results are as follows:
Total sample
Taiwan: 187 persons
Foreign: 70 person





Foreign







Summary

From above information, we can see that the range of 7-12 years old children, school will have a school dentist to check. (Before we asked to the primary school staff, the sample of 36 parents, 93% agreed. And nine schools, all that willing use this product, and will have the intention to let the children with the check), so they think that don’t need to buy this for children.
16 years old will be own regular go to dentist.
19 years old are basically no need. (more factors)
We found that approximately 73% people don’t want to use this product, because they feel only go to dentist enough, and the product is expensive.
On the other hand, foreign people have more high willingness to use this product in home, because they would pay more money to go to dentist, compare this product price, the product is much cheaper.

Communicate with public

Product pamphlet

In order to make the public more convenient and clear understanding of our products, we made a pamphlet for the research and development of products. In the pamphlet, we briefly introduced the use of the product, technology, security and other issues, and tell their in which we used genetically modified technology in the product. We had design a gene that is a kill switch in our gene design, so that when we not only contact to the public in communication can be more convenient to understand the safety of our products, but also help our products Promotion.

Public booth activity

In order to enable our products to actually reach the public, through communication with the public to improve our products, so that products can be more intimate social needs. We cooperate with kumquat farm from Taiwan Chiayi local famous tourist attractions. In the crowd booth, we put our analog product and product pamphlet on our booth, to the public who introduced a lot about our project, including health care teeth of acknowledge, as well as the function of our products and follow-up benefits. Many parents and children after our introduction, not only have a great interest in our products, but also like our exquisite analog device. In which we have received a lot of feedback from the general public, and we have been more understand to their facing problem of oral cavity. Which will help us to improve the product and make the product more in line with the needs of the user!



Improve our product

Visited to dentist

In order to be able to make our products more in line with the actual social needs of the situation, we visited a number of Taiwan Chiayi local dental clinic: 百利、沃爾、邱. In several visits, we understand the actual needs of the patient from the perspective of the dentist and the patient actual oral cavity. We also have a better understanding in the common consumer habits of patients. The information is great help us to plan how to send the product to the public, and what kind of use can make the user the most convenient and greatest help.


Dentist collaboration

In order to enable our data to be accurate, we collaborate with a number of local dentists in Chiayi. Asking them to help the team define our product internal data. When we visited the dentist, we discussed the concept of our product with the dentist, and then we asked whether the dentist could set up the database for the team to build the product. The dentist was very enthusiastic to help us build the product database. We hope to be able to use the dentist's expertise to ensure the accuracy of the product. In which the standard for defining the oral tooth decay are the standards established by the ICDAS (International Caries Detection and Assessment System) organization. This standard is recommended by the students of the dentistry department.



Primary school collaboration

In the course of our contact with the public, we found that the vast majority of parents are willing to buy this product to the children at home, so we turn to visit primary school students in Chiayi, and ask whether there is a willingness to collaborate to use the device. The scope of the user extends to the children. In the interview process, we found that the school current will ask the dentist to school to examine the Taiwanese young children every three months, if the dentist found the children that the oral condition is not good, He will ask the children to see again dentist after child back to home. In this process, the dentist check children oral condition is often too long, causing many inconvenience. Therefore, the school said that if the device in the follow-up to improve the accuracy, then very willing to cooperate with us, hope that through our team of device instead of dentists for children to check the mouth, hoping to easily check the process.

Integrated

Get advises and improve device

After visiting many people, elementary school students and public booth, they are interested in our products and expect that we can continue to develop this product, hoping to improve the accurate so that the future for health care will be a very good product. As a result, we have made the following improvements to our products after we have given us the advice of the public.

  1. To improve the appearance of the device

After we cooperate the local elementary school, they suggested that we can design the device into a lovely and can attract style of children that will increase the use of children willing!

  1. The installation of the slide was originally required to use three slides and was to be installed in three installments. Now change to install once

After we communicate with a lot of public, they feedback that the device is not easy to use. They hope that they can be installed like an ink clip of printer. Therefore, we are going to change the product design. The original product would be used to three slides, and each slide must be installed, the design is too troublesome, so we combined the three slide into one set, the user only install once when he will use it. That is to simplify the use process of the product.

  1. Product appearance of the material from iron to acrylic

When we actual contact with the public and our product form, the vast majority of people said that the sale price of the product is too high. Therefore, we will change the appearance of the product material, from the original appearance of iron changed to acrylic material to reduce the cost of the product, so that product prices can be reduced. 4. Adding an extra space for germinating consumptive material:After we discussed with the local primary school, the health center staff to provide some advices on our products. They are hoping that we can set a space that can placed next to the slide space in the device. The space will prepare to slide that is next one will using in device. When health care personnel are check to mouth of children, which can easy and quickly to take the slide, the design not only easy to manage those flow of slides, but also to avoid the children casually took the slide to cause accident in the inspection. Each class requires the use of three instruments, each of our instruments can be placed in the preparation of slides 8 to 9, a total of about 25 to meet the Taiwanese member of each class.