Line 152: | Line 152: | ||
</p> | </p> | ||
<div class="title-center">Data & Result</div> | <div class="title-center">Data & Result</div> | ||
− | <img style="width:60%; height:auto; src="https://static.igem.org/mediawiki/2017/9/98/T--SCU-WestChina--Wiki-Interlab-Fig1.png" alt=""> | + | <img style="width:60%; height:auto;" src="https://static.igem.org/mediawiki/2017/9/98/T--SCU-WestChina--Wiki-Interlab-Fig1.png" alt=""> |
<div class="img-describe"> | <div class="img-describe"> | ||
Fig. 1 OD600 curve for each replicate, which suggests that most devices had no influence on the replication ability of the cells, while Device 4 and Device 1 showed much lower growth curves. | Fig. 1 OD600 curve for each replicate, which suggests that most devices had no influence on the replication ability of the cells, while Device 4 and Device 1 showed much lower growth curves. | ||
</div> | </div> | ||
− | <img style="width:60%; height:auto; src="https://static.igem.org/mediawiki/2017/9/9f/T--SCU-WestChina--Wiki-Interlab-Fig2.png" alt=""> | + | <img style="width:60%; height:auto;" src="https://static.igem.org/mediawiki/2017/9/9f/T--SCU-WestChina--Wiki-Interlab-Fig2.png" alt=""> |
<div class="img-describe"> | <div class="img-describe"> | ||
Fig. 2 GFP fluorescence data of each device and replicate measured with an excitation of 485 nm and emission of 530 nm | Fig. 2 GFP fluorescence data of each device and replicate measured with an excitation of 485 nm and emission of 530 nm | ||
</div> | </div> | ||
− | <img style="width:40%; height:auto; src="https://static.igem.org/mediawiki/2017/0/0c/T--SCU-WestChina--Wiki-Interlab-Fig3.png" alt=""> | + | <img style="width:40%; height:auto;" src="https://static.igem.org/mediawiki/2017/0/0c/T--SCU-WestChina--Wiki-Interlab-Fig3.png" alt=""> |
<div class="img-describe"> | <div class="img-describe"> | ||
Fig.3 The ratio of fluorescence to growth for each Interlab device. | Fig.3 The ratio of fluorescence to growth for each Interlab device. |
Latest revision as of 17:18, 1 November 2017
Over the past three years, iGEM has advanced the frontiers of science with the biggest interlaboratory studies ever done in synthetic biology. The SCU-WestChina iGEM team is honored to participate in the Interlab study this year.
The aim of the Interlab study is to analyze the measurement data of fluorescence with a detailed protocol, but different lab equipment and environment. In this way, researchers are able to get an access to compare fluorescence data from laboratories all over the world, in a standardized way.
InterLab 2017 Plate Reader Protocol was followed. The device utilized in our Interlab experiments was BioTek Cytation™ 3.
For this study, following constructs are used:
•Negative Control: I20270
•Positive Control: R0040
•Test Device 1: J23101.BCD2.E0040.B0015
•Test Device 2: J23106.BCD2.E0040.B0015
•Test Device 3: J23117.BCD2.E0040.B0015
•Test Device 4: J23101+I13504
•Test Device 5: J23106+I13504
•Test Device 6: J23117+I13504
All devices and parts were obtained from the iGEM 2017 InterLab Distribution Kit. Devices were made with a pSB1C3 plasmid backbone and transformed into Escherichia coli DH5α.
Results from the Plate Reader indicated that the promoter of the Device 4 is strongest, followed by the promoter of the Device 2 and Device 1.
The lowest Intensity was detected in Device 3, Device 5 and Device 6.