Anikasingh (Talk | contribs) |
Anikasingh (Talk | contribs) |
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+ | <div class="tab-pane fade" id="tab7default"> | ||
+ | <div class="media"> | ||
+ | <div class="media-left media-top" style="padding-top:10px"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/9/9e/T--Austin_UTexas_LASA--Week1.jpg" class="media-object" style="width:80px"> | ||
+ | </div> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media-heading">March 1- March 10, 2017</h4> | ||
+ | <p>Week 1: Ran a gel electrophoresis on ppddc and HpaBC. Golden gate reaction of ppddc and ytk001, then purified it. Transformed ppddc + ytk001, both green and white colonies (transformation successful). | ||
+ | |||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="media"> | ||
+ | <div class="media-left media-top" style="padding-top:10px"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg" class="media-object" style="width:80px;height:80px"> | ||
+ | </div> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media-heading">March 13 - March 17, 2017</h4> | ||
+ | <p>Week 2: Miniprepped ppddc + ytk001. Transformed pp2551 from January. Grew up pp2551 for glycerol stocks and Hpabc for miniprep to do a golden gate reaction with ytk001. | ||
+ | |||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="media"> | ||
+ | <div class="media-left media-top" style="padding-top:10px"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg" class="media-object" style="width:80px"> | ||
+ | </div> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media-heading">March 20 - March 24, 2017</h4> | ||
+ | <p>Week 3: Miniprepped HpaBC and nanodropped, but concentrations too low to do a golden gate assembly. Re-did liquid cultures then miniprep of HpaBC. | ||
+ | |||
+ | |||
+ | </p> </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="media"> | ||
+ | <div class="media-left media-top" style="padding-top:10px"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg" class="media-object" style="width:80px"> | ||
+ | </div> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media-heading">March 27 - March 31, 2017 </h4> | ||
+ | <p>Week 4: Figured out/planned how to combine the two separate sensing plasmids (venus cassette and pp2551 cassette). Decided to do multigene assembly using a new backbone. Looked into addition of lac operon in the sensing assembly. | ||
+ | |||
+ | |||
+ | |||
+ | </p> </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div class="tab-pane fade" id="tab8default"> | ||
+ | <div class="media"> | ||
+ | <div class="media-left media-top" style="padding-top:10px"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/9/9e/T--Austin_UTexas_LASA--Week1.jpg" class="media-object" style="width:80px"> | ||
+ | </div> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media-heading">April 1- April 7, 2017</h4> | ||
+ | <p>Week 1: n/a, people out for school. | ||
+ | |||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="media"> | ||
+ | <div class="media-left media-top" style="padding-top:10px"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg" class="media-object" style="width:80px;height:80px"> | ||
+ | </div> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media-heading">April 10 - April 14, 2017</h4> | ||
+ | <p>Week 2: Did a BsaI golden gate reaction for venus cassette plasmid (VCP). Used a backbone (BTK402) with a pMMB67EH origin, ppddc promoter, rpoc terminator, and ConL1 and ConRE connectors. | ||
+ | |||
+ | |||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="media"> | ||
+ | <div class="media-left media-top" style="padding-top:10px"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg" class="media-object" style="width:80px"> | ||
+ | </div> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media-heading">April 17 - April 21, 2017</h4> | ||
+ | <p>Week 3: Dilutions of each part of the venus cassette plasmid. Grew them up to have enough of our own stock. | ||
+ | |||
+ | </p> </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="media"> | ||
+ | <div class="media-left media-top" style="padding-top:10px"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg" class="media-object" style="width:80px"> | ||
+ | </div> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media-heading">April 24 - April 31, 2017 </h4> | ||
+ | <p>Week 4: Did a BsaI golden gate reaction for production assembly (HCP) with a CP25 promoter, HpaBC, rpoc terminator, conLS and conRE connectors. | ||
+ | |||
+ | |||
+ | |||
+ | </p> </div> | ||
+ | </div> | ||
+ | </div> | ||
Revision as of 23:00, 1 November 2017
Notebook
![](https://static.igem.org/mediawiki/2017/9/9e/T--Austin_UTexas_LASA--Week1.jpg)
Sept 1 - Sept 9, 2016
Week 1: Got aliquots of HpaBC from lab, grew up parts (Hpab, HpaC, pp2551) and digested them to check lengths.
![](https://static.igem.org/mediawiki/2017/9/9e/T--Austin_UTexas_LASA--Week1.jpg)
Oct 3- Oct 7, 2016
Week 1: Did PCR to get HpaB out of the HpaBC lab plasmid, sequence verified ppddc in BTK001 backbone, sequence verified pp2551 and HpaC, made glycerol stocks of sequence verified parts
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
Oct 10 - Oct 14, 2016
Week 2: Ordered HpaBC operon from IDT, grew up pEM7 [BTK156], did Gibson assembly of HpaBC in YTK001 entry vector and grew it up
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
Oct 17 - Oct 21, 2016
Week 3: Split parts into different teams, each team working on assembling and sequence verifying individual parts into entry vecotrs[ppddc, HpaBC pEM7, pp2551]
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
Oct 24 - Oct 28, 2016
Week 4: Grew up and miniprepped HpaBC, and pp2551, but transformation of HpaBC did not work.
![](https://static.igem.org/mediawiki/2017/9/9e/T--Austin_UTexas_LASA--Week1.jpg)
Nov 1- Nov 3, 2016
Week 1: Did Gibson assembly, transformed and miniprepped ppddc, pEM7, and HpaBC, found that Gibson assembly for HpaBC did not work
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
Nov 6 - Nov 10, 2016
Week 2: Redid Gibson assembly for HpaBC, realized we were cloning our parts into YTK001 [plasmid vector]
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
Nov 13 - Nov 17, 2016
Week 3: Transformed, miniprepped, made glycerol stocks of HpaBC. Did a gibson assembly of ppddc.
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
Nov 20 - Nov 24, 2016
Week 4: Transformed pp2551, transformed Gibson product of pEM7, transformed and miniprepped ppddc gibson assembly.
![](https://static.igem.org/mediawiki/2017/9/9e/T--Austin_UTexas_LASA--Week1.jpg)
Dec 1- Dec 8, 2016
Week 1: pp2551 plates had no colonies, streaked with glycerol stocks of HpaBC, redid transformation of pp2551
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
Dec 11 - Dec 15, 2016
Week 2: Transformation of HpaBC, ppddc plates had no colonies, redid Gibson assembly for ppddc
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
Dec 18 - Dec 22, 2016
Week 3: miniprepped and made glycerol stocks of HpaBC, got very low concentrations for the minipreps, looked into origin of the backbone (BTK001). Transformed gibson reaction of ppddc, plate had no colonies.
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
Dec 25 - Dec 29, 2016
Week 4: Digested ppddc and backbone (BTK001) to see if there was a problem in the ori, ran on a gel, showed no insert.
![](https://static.igem.org/mediawiki/2017/9/9e/T--Austin_UTexas_LASA--Week1.jpg)
Jan 1- Jan 5, 2017
Week 1: Used Zymo DNA clean up kit to get more concentrated values to send in for sequencing. Established plan for how to proceed with each part.
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
Jan 8 - Jan 12, 2017
Week 2: Miniprepped pp2551. Designed primers for ppddc pcr to add golden gate overhangs.
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
Jan 15 - Jan 19, 2017
Week 3: Sent in HpaBC and pp2551 to sequencing. Started ‘lab trainings’ with highschool students new to the club.
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
Jan 22 - Jan 26, 2017
Week 4: Received primers for ppddc, and diluted them.
![](https://static.igem.org/mediawiki/2017/9/9e/T--Austin_UTexas_LASA--Week1.jpg)
Feb 1- Feb 9, 2017
Week 1: Ran a PCR reaction of ppddc to add golden gate overhangs.
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
Feb 12 - Feb 16, 2017
Week 2: Received and grew up an aliquot of new backbone (YTK001) with ColE origin because the original backbone was giving us trouble with getting high enough concentrations to sequence verify it.
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
Feb 19 - Feb 23, 2017
Week 3: Miniprepped new backbone (YTK001). Did a PCR clean-up of ppddc.
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
Feb 26 - Feb 28, 2017
Week 4: Re-ran PCR reaction and purified ppddc because we were running low.
![](https://static.igem.org/mediawiki/2017/9/9e/T--Austin_UTexas_LASA--Week1.jpg)
March 1- March 10, 2017
Week 1: Ran a gel electrophoresis on ppddc and HpaBC. Golden gate reaction of ppddc and ytk001, then purified it. Transformed ppddc + ytk001, both green and white colonies (transformation successful).
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
March 13 - March 17, 2017
Week 2: Miniprepped ppddc + ytk001. Transformed pp2551 from January. Grew up pp2551 for glycerol stocks and Hpabc for miniprep to do a golden gate reaction with ytk001.
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
March 20 - March 24, 2017
Week 3: Miniprepped HpaBC and nanodropped, but concentrations too low to do a golden gate assembly. Re-did liquid cultures then miniprep of HpaBC.
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
March 27 - March 31, 2017
Week 4: Figured out/planned how to combine the two separate sensing plasmids (venus cassette and pp2551 cassette). Decided to do multigene assembly using a new backbone. Looked into addition of lac operon in the sensing assembly.
![](https://static.igem.org/mediawiki/2017/9/9e/T--Austin_UTexas_LASA--Week1.jpg)
April 1- April 7, 2017
Week 1: n/a, people out for school.
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
April 10 - April 14, 2017
Week 2: Did a BsaI golden gate reaction for venus cassette plasmid (VCP). Used a backbone (BTK402) with a pMMB67EH origin, ppddc promoter, rpoc terminator, and ConL1 and ConRE connectors.
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
April 17 - April 21, 2017
Week 3: Dilutions of each part of the venus cassette plasmid. Grew them up to have enough of our own stock.
![](https://static.igem.org/mediawiki/2017/d/d6/T--Austin_UTexas_LASA--Month.jpg)
April 24 - April 31, 2017
Week 4: Did a BsaI golden gate reaction for production assembly (HCP) with a CP25 promoter, HpaBC, rpoc terminator, conLS and conRE connectors.
What should this page have?
- Chronological notes of what your team is doing.
- Brief descriptions of daily important events.
- Pictures of your progress.
- Mention who participated in what task.
Inspiration
You can see what others teams have done to organize their notes: