Have you already chosen a protein to be evolved?
You already accomplished the first and most important step. Let’s see whether you
are ready to evolve it!
No problem, there are lots of proteins waiting to be evolved.Go on to find
out what you should take care of choosing your protein and preparing your
experiments. Maybe have a look at our toolbox to get an idea of the wide range
of proteins you can evolve with
PREDCEL
Which approach do you pursue by using our evolutionary toolbox?
Did you discuss your idea with a medical professional? We are sure this expertise
would be valuable to choose the right experimental settings.
Did you consider that your products might be dangerous? To get to know this, you
could engage a chemical expert.
Be aware that a number of countries have strict regulations regarding green
biotechnology.
That sounds quite uncertain. Before you start or pursue evolving your protein, you
should know the exact purpose and impact. How about engaging with your local
community to figure out possible needs?
Engage with the community in the field of in vivo evolution – we´d be interested in
discussing your project.
Are you sure your protein is safe to be evolved?
Nevertheless we advise you to check it in our SafetyNet, there could be “sleeping”
hazardous potential.
Do you know which equipment is required to safely work with phages?
Did your considerations include that phages are very volatile? You should create a
special “phage space” in your lab to reduce contamination. Furthermore, you should
comply with the safety level of your evolving protein. Check the safety level of your
protein on our SafetyNet
Check advices on our safety page and get in touch with your safety representative
Before performing your PREDCEL experiments you should reflect on the
consequences
Did you calculate all required parameters on our Integrated Modeling page?
Well done! Now you have the optimal conditions to succeed
Visit our Integrated Modeling page to figure out all required parameters for a
successful PREDCEL run
You should consider that our Integrated Modeling reduces effort, costs and
environmental burden
Did you already engage with your local community to discuss your plans?
Fine. For more ideas, how to engage with the public you could watch the expert interview
with Dr. Kaufmann
You should integrate public needs to ensure the beneficial impact for humanity of your
research aim
You should first engage with your local community to discuss concerns before using our
PREDCEL toolbox
Did you consider possible consequences of your planned PREDCEL experiment?
You are a responsible scientist. We encourage you to use our PREDCEL toolbox and convey
the spirit of responsible science to your friends and colleagues
Take your time to reflect and research on possible consequences to prevent endangering the
environment and yourself. You should engage with experts in this field
Before starting your experiment, you should consult experts and their recommendations
Now it´s on you!
When you talk about PREDCEL with your colleagues, friends and local
communities, you can spread the idea of making directed evolution experiments safe and to
use it responsibly for addressing urgent human needs