Lab
Characterisation of BBa_J04450 and BBa_K801100:
This year, we worked on BBa_J04450 and BBa_K801100 and searched the importance of IPTG induction on the production of mRFP1 so as to use this part in our kit.
It was essential for us to know if there is an importance of the inducer in RFP production. A strong leak of RFP production is observed and could be seen on appropriate wavelength.
A : Tubes used for B&C photos. Photos were taken with SnapLab, the detection kit made by Grenoble-Alpes iGEM Team (see pictures below).
We focused our research on the estimation of a difference of fluorescence intensity and a quicker induction, if it exists.
Results showed IPTG had an importance in RFP production. Indeed, we can see that IPTG allow an augmentation of RFP after a decrease period.
Results showed IPTG had an importance in RFP production. Indeed, we can see that IPTG allow an augmentation of RFP after a decrease period. We design this experience with an unique IPTG concentration, however, it could be interesting to repeat this experience with different IPTG gradient.
