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...(str,units){var c,hi,lo;var byteArray=[];for(var i=0;i<str.length;++i){if((units-=2)<0)break;c=str.charCodeAt(i);hi=c>>8;lo=c%256;byteArray.push(lo);byteArr ...+this.slice_end+"\n";string+="Slice Code = "+this.slice_code+"\n";string+="Units Code = "+this.xyzt_units+" ("+this.getUnitsCodeString(nifti.NIFTI1.SPATIAL_

<td class="tg-yw4l">2,5 units/50µL PCR</td> <td class="tg-yw4l">1,25 units/25µL PCR</td>

...r incubation time had the greatest effect on tRNA cleavage efficiency with units of RNase H being optimal between the range of 5-50. With these improvements ...2 fraction 1 20 units of RNase H added; 4- concentrated MS2 fraction 2 20 units of RNase H added; 5- tRNA standard (76 nt).

<h3 class=leap>Safety Case Units and Structure:</h3> ...single root Goal (usually stating that some project is safe) with Context units for the intended environmental conditions of the organism and the species a

...<p><b>Figure 2.</b> Flow cytometric fluorescence measurements in arbitrary units as a function of time. Left: Cultures were grown in LB medium. Right: Cultu ...combined into a single device containing a compartment for each of the two units</span>. Details about the construction and device will be discussed in the

<td>1.0 units/50µL PCR</td> <td>1.25 units/50µL PCR**</td>

...combined into a single device containing a compartment for each of the two units</span>. Details about the construction and device will be discussed in the <p><span class="highlighted">Our device is composed of two units, an energy storing unit and an energy converting unit</span>, each divided

First we characterized the first translated units of the aaRS-test systems by transforming them solely into <i>E.coli</i> BL2 ...he intensity of the emitted light of the two different fluorescent protein units to determine if there were any differences. To do so we took care that the

...ase H added; 7- MS2 fraction 10 units of RNase H added; 8- tRNA elution 10 units of RNase H added; 9- tRNA standard (76 nt).</p>

...nes from IDT and pHYG3 ChrR+Chromodulin were on our hands. Transcriptional units from IDT were ligated with pChlamy_4 vector which possesses an endogenous p ...<li>PCR of transcriptional units from IDT didn’t work because of low quality DNA of ChrR and Chromodulin d

...d pathway in the <em>T.atroviride</em> and to integrate more transcription units to the genome of the <em>T.atroviride</em> at the same time. Therefore, we .... Thus, two transcription units were assembled. Then the two transcription units were transformed into <em>T.atroviride</em> for assembly and integration wi

''At least one enzyme has < 100% activity in this buffer, so additional units of enzyme and/or longer incubation time may be necessary.'' *Recommended maximum of 1 µg of substrate per 10 units of enzyme.

...To be able to calculate the interaction energies between different protein units, the hydrophobic moment and isoelectric point (<a href="#references" target ...protein x-ray structures. This means that they are not applicable for the units in our models, which contain five amino acid residues each.

<td>1.25 units/50 µl PCR</td> <td>1.25 units/50 µl PCR</td>

...osan oligomers from chitin by deacetylating the <i>N</i>-acetylglucosamine units of the substrate&nbsp;<a href="#[3]">[3]</a>. During deacetylation, acetic ...>. Deacetylation describes hydrolysis of the acetamido group of the GlcNAc units, thus generating acetic acid und D-glucosamine&nbsp;(GlcN)&nbsp;<a href="#[

<td>1.25 units/50 µl PCR</td> <td>1.25 units/50 µl PCR</td>

...benthamiana. Using golden gate cloning, we are generating transcriptional units for transient expression of TSHantag after agrobacterium-mediated transform ...ated that functional peptide domains can be fused to the self-polymerizing units of curli to form a variety of functional materials. Thus, curli fibers pres

...s can be changed for any other molecule you are trying to inhibit, and the units can therefore be changed to. <br><br> ...ient, but should only be used when trying to calculate the total number of units of 'I' in a given volume of liquid. <br><br>

...osan oligomers from chitin by deacetylating the <i>N</i>-acetylglucosamine units of the substrate&nbsp;<a href="#[3]">[3]</a>. During deacetylation, acetic ...>. Deacetylation describes hydrolysis of the acetamido group of the GlcNAc units, thus generating acetic acid und D-glucosamine&nbsp;(GlcN)&nbsp;<a href="#[

...a cell density of 1 OD600. Therefore, the total cell volume is 1000 volume units. As the volume unit is defined as the volume of a single cell, there are a ...ween 0 and 10. These parameter sets were simulated for a total of 480 time units, where between t=240 and t=300, the external signal S was set from 0 to 1.

...TOP10 <i>Escherichia coli</i> chassis, measured as Arbitrary Fluorescence Units/OD₆₀₀. The GFP expression levels were compared in LB media and m ...</i> chassis in LB media and SOC media, measured as Arbitrary Fluorescence Units/OD₆₀₀. The GFP expression levels were measured every hour over a

...scence data usually cannot be compared because it is reported in different units or because different groups process data in different ways. ...chers with a detailed protocol and data analysis form that yields absolute units for measuring GFP in a plate reader.

<p>The next step in analyzing the data is to determine how many units of monochlorodimedone can be brominated per milliliter of enzyme. One unit ...his calculation was done the cell lysate was found to be converting 0.0043 units of monochlorodimedone per milliliter of enzyme. This proves that the enzyme

...ss="myButton" style="color:#171A3D;width:100%;">Relating Relative Promoter Units to Transcription Rate</button> ...pan style="font-weight:normal; font-size: 23pt">Relating Relative Promoter Units to Transcription Rate</span></center></font></h1><hr/>

...ss="myButton" style="color:#171A3D;width:100%;">Relating Relative Promoter Units to Transcription Rate</button> ...pan style="font-weight:normal; font-size: 23pt">Relating Relative Promoter Units to Transcription Rate</span></center></font></h1><hr/>

...is the mean of 3 biological repeats. RFU stands for relative fluorescence units.</center> ...nserting two different sensitivity tuner constructs between the processing units of the Sensynova platform; <a href="http://parts.igem.org/Part:BBa_K274371"

<th align="left">Units</th> <td>adapted to fit units from [2]</td>

...ulation to assist in recombinase characterization. The behavior of control units control units under recombinase-mediated sequence inversion was predicted with stochastic

considered developing two hierarchical execution units supporting our Bio-Flip-Flop. We called these two units the forward latch and the backward latch. The state transition process of e

...hite background signifies the day. Fumarate and glycogen are both given in units of mmol L ...xis is flux through ICL. The color indicates either the growth rate (A) in units hr

...is the mean of 3 biological repeats. RFU stands for relative fluorescence units.</center> ...nserting two different sensitivity tuner constructs between the processing units of the Sensynova platform; <a href="http://parts.igem.org/Part:BBa_K274371"

<th>Units</th> <th>Units</th>

...y of 1.0 OD₆₀₀. Therefore, the total cell volume is 1000 volume units. As the volume unit is defined as the volume of a single cell, there are a ...on from pyDOE. These parameter sets were simulated for a total of 240 time units, where between t=120 and t=180, the external signal S was set from 0 to 1.

...rtain distance in the farmland while main device needs only one. The slave units can transform information to main device through 2.4G data transmission mod <p class="PP">Slave units consist of plants' status of health monitoring device---an e-nose system. I

<td width="113" valign="top"><p><strong>Units</strong></p></td> <td width="142" valign="top"><p><strong>Units</strong></p></td>

<td width="142" valign="top"><p><strong>Units</strong></p></td> <td width="95" valign="top"><p><strong>Units</strong></p></td>

...iptional units were ordered from IDT company using iGEM promotion. These 4 units were designed (additional flanking 24 bp sequences) in a such way so that i <p>As it can be seen, these 4 transcriptional units have overlapping regions of the length of 49 bp each for Gibson Assembly (F

...ulation 1 - absence of oxidized electron carriers halts ammonia oxidation. Units of concentration: (mmol NH₃)/L, time is in hours. Note the short ...entrations of nitrogen with (blue) and without (yellow) ammonia oxidation. Units of concentration: (mmol N₂)/L, time is in hours</p></figcaption>

...the two controls across time points of 0, 2, 4 and 6 hours. AU= arbitrary units}}</center> ...the two controls across time points of 0, 2, 4 and 6 hours. AU= arbitrary units}}</center>

...d lots AH01, AB01) to allow for setting independent conversion to absolute units. ...was used for downstream analysis and conversions to absolute fluorescence units. The two

...<p> From the figure, we can see lot of things. In the first 50 units of time, most Reebot quickly enriched enough lanthanide ions, the concentra ...ick reaction, capturing the lanthanide ion and recycle. When it passes 100 units of time, other lanthanide ion release at another position in bottom. Reebot

...zed to p-nitrophenol (ε405=17,700 M-1 cm-1). Activities were expressed as units (1 μmol of p-nitrophenol formed per minute) per OD600 whole cells. ...r bars, mean ± SD (n=3 samples) from the same experiment. a.u., arbitrary units.

...erma Homologous Recombination Kit</a> we providing, two dual transcription units, homologous arm-URA3 marker-L1-tRPS2-phlA-TEF2-PGK1-phlB-tTDH1-L2(<a class= ...<p class="PP capture">Fig.5 Structure of two dual transcription units</p>

...” cells as cells having a fluorescence of greater than 1000 fluorescence units.</p> ...together on the same day. Average fluorescence is provided in fluorescence units as measured by the flow cytometer. Additionally, the average fluorescence p

...[3]</a>. Chitinases break down the glycosidic bonds between chitin monomer units and are classified as hydrolases. ...sm of ChiA1.</b> ChiA1 breaks down the glycosidic bonds between the GlcNAc units of a chitin pentamer, thus creating chitin dimers. </figcaption> </center>

...(Figure 11 and 12) are based on this standard curve. RLU = relative light units.</a> ...and the error bars represent the standard deviations. RLU = relative light units.</a>

...is to determine how we can improve our system by choosing other regulatory units. ....</strong> The timing diagram of tryptophan over Im2 expression (arbitrary units). The functional model has been generated by using Tryptophan operator with

<th>Units</th> ...with a size of 1-2 μm, 1 molecule in the cell = 1nm. Thus we convert all units to be measured in molecules, as this fits the premises of the gillespie alg

<th class="tg-031e">Units</th> <th class="tg-yw4l">Units</th>

...duct concentration) can be plotted as a function of T1. With our arbitrary units of time, the plot appears as in figure 9. ...ion time as a function of incubation time of function 1 (In arbitrary time units)</figcaption>

...body has an infective titer of 9.6*10¹¹ CFU/mL (colony forming units per mL). We visualized these phages using transmission electron microscopy ...brary has an infective titer of 5.7*10¹⁵ CFU/mL (colony forming units per mL). This library can then be used to screen for the appropriate affibo

...tive, and a negative control) in expressing GFP and measuring it in common units to compare results across the world and across a variety of plate readers.< ...calculating a correction factor to convert our data into the standardized units that iGEM requested (OD<subscript>600</subscript>).

...rity, we have taken the half-life of transient complex as 5 arbitrary time units and that of the substrate-mediated complex is set to 100. ...irements. The aim of this year’s study was to produce common, comparable units for measuring green fluorescence protein (GFP) with different plate readers

...r c=e("./delimiter");var d=x(c);var v=e("./domTree");var h=x(v);var p=e("./units");var m=e("./utils");var g=x(m);var b=e("./stretchy");var y=x(b);function x ...:89,"./buildCommon":91,"./delimiter":97,"./domTree":98,"./stretchy":110,"./units":114,"./utils":115,"babel-runtime/core-js/json/stringify":5}],93:[function(

...ay, periplasmic binding protein, and degreening parts into transcriptional units.<br><h4><b>Protocol:</b></h4><br>Minipreps were conducted using iGEM lab ma

...tion in the LasR and Lux AHL system is expected to be around 2-6 arbitrary units. This unexpected point is also present in the TraR and Rhl AHL system. The ...LasR and Sin AHL. The 1E-7M expresses a higher GFP of around 20 arbitrary units than the 1E-4M, which is an unexpected result. This is beneficial to resear

...uidic channels lined by living human cells, which replicate key functional units of living organs to reconstitute integrated human organ-level pathophysiolo ...lism in the hepatic triad of the liver, etc.). Typically, these functional units comprise different types of specialized tissues (e.g., epithelium, vascular

...(Figure 11 and 12) are based on this standard curve. RLU = relative light units.</a> ...and the error bars represent the standard deviations. RLU = relative light units.</a>

// Trust units reported by jQuery.css * Check if two date units are the exact.

measure fluorescence in units of equivalent fluorescein concentrations. It fits in a pipette box and cost ...ty from the fit of <i>k</i>. We are now enabled to measure fluorescence in units of equivalent fluorescein concentrations <i>c</i>. We analysed data of a fi

...run this protocol and to test dilution factors for counting colony forming units. The entire method for protocol was summarized to smooth out logistics. ...llaboration. Katie received info for the researcher feature. A search for units used for FBA/FVA was started to apply to the mathematical model. Gel extra

...aimed to quantify their expression levels using luciferase transcriptional units and also use them to generate a thyroid stimulating hormone antagonist that ...antag protein. Therefore, we will design and create unique transcriptional units (TUs) for expression of TSHantag in tobacco. These TUs will be introduced i

...scence data usually cannot be compared because it is reported in arbitrary units or different groups process data in different ways. Often we work around th ...ers with a detailed protocol and data analysis format that yields absolute units for measuring GFP in a plate reader.

...gram of polymers with effective chain lengths of (A) 400 units and (B) 100 units. The parameters $\phi$ and $\sigma$ denote the volume fraction of the solut

...he time point of 4 hours. Error bars are standard deviation. AU= arbitrary units}}</center> ...the dimers in DH5&alpha;. Error bars are standard deviation. AU= arbitrary units}}</center>

...<p class="text-content-new">The system consists of four essential units: a transmembrane domain, a photocleavable linker, dCas9 and a transactivati ...lass="text-content-new">We designed and tested two different transcription units (TU) containing a CMV promoter (BBa_K747096), a signal peptide with cleavag

...ing are different. The goal of the InterLab Study is to determine absolute units for measuring GFP that are comparable despite the type of plate reader used

...o do so, the construct containing the E-PIF6 and PhyB-VP16 transcriptional units was transformed, together with the chimeric promoter nearby the luciferase ...o do so, the construct containing the E-PIF6 and PhyB-VP16 transcriptional units was transformed, together with the chimeric promoter nearby the luciferase

...pan style="font-weight:normal; font-size: 23pt">Relating Relative Promoter Units to Transcription Rate</span></center></font></h1><hr/> ...umber of RNAP molecules that clear the final base pair of a promoter (with units of [PoPS]

.units{ float:left } <!--drop down menu input conc units-->

...<td style='border:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>2,5 units/50µL PCR</td> ...<td style='border:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>2,5 units/50 µL PCR

...is simply the <b>concentration of bacteria produced per unit time</b>, the units are:</p> <p>Now we have all the variables needed to cross out the units, thus we can solve it as:</p>

<td>10 – 20 units</td> <td>appropriate enzyme or enzymes</td> <td>5 units</td>

...r than the control group by 17 units with no induction and is higher by 21 units with 100-μM-Cu induction. By aligning the sequence with the CUP1 promoter,

...positive control 1 and the two samples. Furthermore, the bands of the GFP-units can be seen at ~ 27 kDa and the bands of the 2-NPA-RS at ~ 35 kDa. At the b ...firmed our expectations. The bands of the whole fusion protein, of the GFP-units and the cleaved streptavidin-tags are clear to see. The GFP-bands are very

...ments and comparing the results to a measured standard curve, the relative units of fluorescence can be converted to absolute units. We started our contribution by resuspending the appropriate DNA from the i

...andard fluorescence measurement protocol and the use of common, comparable units for fluorescence.

<p>Our sensing construct consisted of two primary transcriptional units: one with the pp2551 coding gene and one with the Venus fluorescent protein Here is a brief overview of the transcriptional units and their functions.

and two separate measuring units, one of which is the OD our measure units. Further to obtain a steady OD

...r than the control group by 17 units with no induction and is higher by 21 units with 100-μM-Cu induction. By aligning the sequence with the CUP1 promoter,

<td>Translational units</td> <td>Translational units</td>

...ns because of shorter chain lengths and free amino groups in D-glucosamine units. Formerly described physico-chemical properties also have a large impact on

* Check if two date units are the exact. * Check if two date units overlap.

* Check if two date units are the exact. * Check if two date units overlap.

...font-size:18px"> We choose BBa_J23100(figure 4) from the Relative Promoter Units (RPUs) as the comparison to the sacB promoter. Three groups were set includ ...two parallel groups were attained. According to the arithmetic in terms of units, we divided it by the OD600 also averaged from each of the parallel groups.

3) Measure the DNA concentration on Nanodrop in units of ng/µL. 2 µL was used for each measurement<br> ...ill to each pellet. Add 500 µL lysozyme (10 mg/ml) and 2 µL DNAse I (238 units/µL). Put on stirring at 4 °C for 40 min.<br>

...fore to improve current measurement tools by converting data into absolute units.</p>

...nM. The detector is able measure to automatically measure fluorescence in units of equivalent fluorescein concentrations. The detector is very compact and ...\sigma_k^2}.</script></span> We are now enabled to measure fluorescence in units of equivalent fluorescein concentrations <span class="math inline"><span cl

...nterLab Study in order to provide protocols for users to generate absolute units for measuring GFP. This is the fourth year iGEM has done the <a href="#">In

plating to compare the amount of colony forming units on LB versus LB-Kanamycin (Figure 3). Unexpectedly we noticed <br><br>Yamamoto et. Al saw at 15-fold decrease in colony forming units with the addition of <i>ietAS</i> with the

GQ(Tq(),a,"units",tX());if(nq(S)){S=S.ha;Tq();tqa||(tqa=(new BX).b());a=GQ(0,a,"direction",t ...S=NX(),S=tv(S,a.vm),S=(new A).e("step",S),U=JX(),U=tR(U,a.np),U=(new A).e("units",U);Lqa||

...on quantifying the expression of GFP in common, <br>comparable or absolute units.<br> ...fferent labs. The main task was to quantify expression of GFP in different units. In our case, we measured fluorescence using a plate reader.<br><br>

...ers with a detailed protocol and data analysis form, which yields absolute units to measure GFP in a plate reader.

...matic of the translational units designed to visualize Cpx activation. All units are under control of the inducible pBad/araC promoter and strong RBS BBa_B0

...lar experiment, the intensities of GFP fluorescence (Relative Fluorescence Units; RFU) have shown to follow Hill's equation (2). Therefore, in this study, t ...="font-size: 16px">Fig. 4 Concentration dependance of Reletive Fluoroscent Units </figcaption>

...general expression <b>Evaluation Vector (EV)</b> with easily exchangeable units: I) allowing the replacement of the promoter (which drives the system) and <li>Insertion of basic or composite parts as expression units</li>

...moter and Lac operator to control Mtr CAB’s expression. Both of this two units are induced by IPTG. With this dual switch, we can reduce the leak of the g ...romoter and Lac operator to control KMADH’s expression. Both of this two units are induced by IPTG. With this dual switch, we can reduce the leak of the g

...\text{Sr.} & \text{Parameter} & \text{Description} & \text{value} & \text{units} \\ \hline ...\text{Sr.} & \text{Parameter} & \text{Description} & \text{value} & \text{units} \\ \hline

...nterLab Study in order to provide protocols for users to generate absolute units for measuring GFP. This is the fourth year iGEM has done the <a href="https

...<td>colony forming units for <i> E. coli</i> (cfu) or plaque forming units (pfu) for M13 phage</td>