This page is different to the "Applied Design Award" page. Please see the <a href="https://2017.igem.org/Team:Austin_UTexas_LASA/Applied_Design">Applied Design</a> page for more information on how to compete for that award.
Part of our project revolved around an assembly that produced L-DOPA (levadopa or L-3,4-dihydroxyphenylalanine) from glucose.
We constructed our production assembly by cloning a CP25/PA3-hpaBC-rpoc transcriptional unit into a vector containing a --- ori and spectinomycin resistance marker. Our construct was assembled using golden gate cloning techniques.
Diagram here.
Here is a brief overview of each main constituent of the hpaBC transcriptional unit:
CP25 promoter (BBa_---): strong promoter; initiates transcription of gene expressing hpaBC; interchanged with PA3 promoter.
PA3 promoter (BBa_---): moderately-strong promoter; initiates transcription of gene expressing hpaBC; interchanged with CP25 promoter.
hpaBC (BBa_K2458000): gene expressing the enzyme hpaBC (4-hydroxyphenylacetate-3-hydrolase). Add more info here? **
The second half of our project revolved around an assembly that sensed the production of L-DOPA.
Our sensing construct consisted of several ---. We constructed this assembly using golden gate, gibson, and traditional cloning techniques.
Diagram here.
Here is a brief overview of the transcriptional units and their functions.
lacI Transcriptional Unit -
pp2551 Transcriptional Unit - expresses transcriptional factor pp2551 in the presence of pDOPA.
