Difference between revisions of "Team:Austin UTexas LASA/Design"
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Here is a brief overview of each main constituent of the hpaBC transcriptional unit: | Here is a brief overview of each main constituent of the hpaBC transcriptional unit: | ||
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| − | <li>CP25 promoter (BBa_---): strong promoter; initiates transcription of gene expressing hpaBC; interchanged with PA3 promoter. | + | <li><p>CP25 promoter (BBa_---): strong promoter; initiates transcription of gene expressing hpaBC; interchanged with PA3 promoter. |
| − | <li>PA3 promoter (BBa_---): moderately-strong promoter; initiates transcription of gene expressing hpaBC; interchanged with CP25 promoter. | + | <li><p>PA3 promoter (BBa_---): moderately-strong promoter; initiates transcription of gene expressing hpaBC; interchanged with CP25 promoter. |
<li>hpaBC (BBa_K2458000): gene expressing the enzyme hpaBC (4-hydroxyphenylacetate-3-hydrolase). Add more info here? ** | <li>hpaBC (BBa_K2458000): gene expressing the enzyme hpaBC (4-hydroxyphenylacetate-3-hydrolase). Add more info here? ** | ||
<li>rpoc terminator (BBa_K2458001): --- terminator; terminates transcription of gene expressing hpaBC. ** | <li>rpoc terminator (BBa_K2458001): --- terminator; terminates transcription of gene expressing hpaBC. ** | ||
Revision as of 17:20, 1 November 2017
Design
Production Plasmid
Part of our project revolved around an assembly that produced L-DOPA (levadopa or L-3,4-dihydroxyphenylalanine) from glucose.
We constructed our production assembly by cloning a CP25/PA3-hpaBC-rpoc transcriptional unit into a vector containing a --- ori and spectinomycin resistance marker. Our construct was assembled using golden gate cloning techniques.
Diagram here.
Here is a brief overview of each main constituent of the hpaBC transcriptional unit:
CP25 promoter (BBa_---): strong promoter; initiates transcription of gene expressing hpaBC; interchanged with PA3 promoter.
PA3 promoter (BBa_---): moderately-strong promoter; initiates transcription of gene expressing hpaBC; interchanged with CP25 promoter.
Sensing Plasmid
Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.This page is different to the "Applied Design Award" page. Please see the Applied Design page for more information on how to compete for that award.
What should this page contain?
- Explanation of the engineering principles your team used in your design
- Discussion of the design iterations your team went through
- Experimental plan to test your designs
Inspiration
