Difference between revisions of "Team:Austin UTexas LASA/Design"

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<p style="font-size:1.6em;color:black;margin-bottom:5px"><b>Sensing Plasmid</b></p>
 
<p style="font-size:1.6em;color:black;margin-bottom:5px"><b>Sensing Plasmid</b></p>
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Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
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The second half of our project revolved around an assembly that sensed the production of L-DOPA.
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Our sensing construct consisted of several ---. We constructed this assembly using golden gate, gibson, and traditional cloning techniques.
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Diagram here.
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<br></br>
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Here is a brief overview of the transcriptional units and their functions.
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lacI Transcriptional Unit -
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<li>pp2551 Transcriptional Unit - expresses transcriptional factor pp2551 in the presence of pDOPA.  
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<li>CP25 promoter (BBa_---): strong promoter; initiates transcription of gene expressing pp2551.
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<li>pp2551 (BBa_K2458003): gene expressing the transcriptional factor pp2551. Add more info here? **
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<li>ilvGEDA terminator (BBa_K2458001): --- terminator; terminates transcription of gene expressing hpaBC. **
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<li>Venus Transcriptional Unit -
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<li>ppDDC promoter (BBa_K2458007):
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<li>Venus fluorescent protein (BBa_---):
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<li>rpoc terminator (BBa_K2458001):
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Revision as of 17:21, 1 November 2017


Design

Production Plasmid

Part of our project revolved around an assembly that produced L-DOPA (levadopa or L-3,4-dihydroxyphenylalanine) from glucose.

We constructed our production assembly by cloning a CP25/PA3-hpaBC-rpoc transcriptional unit into a vector containing a --- ori and spectinomycin resistance marker. Our construct was assembled using golden gate cloning techniques.

Diagram here.

Here is a brief overview of each main constituent of the hpaBC transcriptional unit:

  • CP25 promoter (BBa_---): strong promoter; initiates transcription of gene expressing hpaBC; interchanged with PA3 promoter.
  • PA3 promoter (BBa_---): moderately-strong promoter; initiates transcription of gene expressing hpaBC; interchanged with CP25 promoter.
  • hpaBC (BBa_K2458000): gene expressing the enzyme hpaBC (4-hydroxyphenylacetate-3-hydrolase). Add more info here? **
  • rpoc terminator (BBa_K2458001): --- terminator; terminates transcription of gene expressing hpaBC. **

    • Sensing Plasmid

    The second half of our project revolved around an assembly that sensed the production of L-DOPA.

    Our sensing construct consisted of several ---. We constructed this assembly using golden gate, gibson, and traditional cloning techniques.

    Diagram here.

    Here is a brief overview of the transcriptional units and their functions.

    lacI Transcriptional Unit -
  • pp2551 Transcriptional Unit - expresses transcriptional factor pp2551 in the presence of pDOPA.
  • CP25 promoter (BBa_---): strong promoter; initiates transcription of gene expressing pp2551.
  • pp2551 (BBa_K2458003): gene expressing the transcriptional factor pp2551. Add more info here? **
  • ilvGEDA terminator (BBa_K2458001): --- terminator; terminates transcription of gene expressing hpaBC. **
  • Venus Transcriptional Unit -
  • ppDDC promoter (BBa_K2458007):
  • Venus fluorescent protein (BBa_---):
  • rpoc terminator (BBa_K2458001):

    This page is different to the "Applied Design Award" page. Please see the Applied Design page for more information on how to compete for that award.

    What should this page contain?
    • Explanation of the engineering principles your team used in your design
    • Discussion of the design iterations your team went through
    • Experimental plan to test your designs
    Inspiration

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