Team:Austin UTexas LASA/Notebook
Notebook
Sept 1 - Sept 9, 2016
Week 1: Got aliquots of HpaBC from lab, grew up parts (Hpab, HpaC, pp2551) and digested them to check lengths.
Oct 3- Oct 7, 2016
Week 1: Did PCR to get HpaB out of the HpaBC lab plasmid, sequence verified ppddc in BTK001 backbone, sequence verified pp2551 and HpaC, made glycerol stocks of sequence verified parts
Oct 10 - Oct 14, 2016
Week 2: Ordered HpaBC operon from IDT, grew up pEM7 [BTK156], did Gibson assembly of HpaBC in YTK001 entry vector and grew it up
Oct 17 - Oct 21, 2016
Week 3: Split parts into different teams, each team working on assembling and sequence verifying individual parts into entry vecotrs[ppddc, HpaBC pEM7, pp2551]
Oct 24 - Oct 28, 2016
Week 4: Grew up and miniprepped HpaBC, and pp2551, but transformation of HpaBC did not work.
Nov 1- Nov 3, 2016
Week 1: Did Gibson assembly, transformed and miniprepped ppddc, pEM7, and HpaBC, found that Gibson assembly for HpaBC did not work
Nov 6 - Nov 10, 2016
Week 2: Redid Gibson assembly for HpaBC, realized we were cloning our parts into YTK001 [plasmid vector]
Nov 13 - Nov 17, 2016
Week 3: Transformed, miniprepped, made glycerol stocks of HpaBC. Did a gibson assembly of ppddc.
Nov 20 - Nov 24, 2016
Week 4: Transformed pp2551, transformed Gibson product of pEM7, transformed and miniprepped ppddc gibson assembly.
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- Chronological notes of what your team is doing.
- Brief descriptions of daily important events.
- Pictures of your progress.
- Mention who participated in what task.
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