Difference between revisions of "Team:Austin UTexas LASA/Results"
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| − | + | Our team did not have an assay to get quantitative measurements concerning the production of L-DOPA in our production assembly. However, by itself, L-DOPA will oxidize to melanin. As a result, to get qualitative results concerning our production assembly, we grew up our production cells in LB media containing 5 mM tyrosine and 0 mM tyrosine and then observed if a color change occurred. The diagram above shows such a color change. Our production assembly did observe a similar color change, but the change in color was not very pronounced. | |
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Revision as of 03:42, 18 November 2017
Results
Overview
- Assembled and sequence verified Venus cassette plasmid and pp2551 cassette plasmid
- Assembled the multigene sensing assembly
- Tested another version of the sensing assembly and demonstrated that it works
Production Circuit
Our team did not have an assay to get quantitative measurements concerning the production of L-DOPA in our production assembly. However, by itself, L-DOPA will oxidize to melanin. As a result, to get qualitative results concerning our production assembly, we grew up our production cells in LB media containing 5 mM tyrosine and 0 mM tyrosine and then observed if a color change occurred. The diagram above shows such a color change. Our production assembly did observe a similar color change, but the change in color was not very pronounced.
- Assembled the multigene sensing assembly
- Tested another version of the sensing assembly and demonstrated that it works
Production Circuit
Our team did not have an assay to get quantitative measurements concerning the production of L-DOPA in our production assembly. However, by itself, L-DOPA will oxidize to melanin. As a result, to get qualitative results concerning our production assembly, we grew up our production cells in LB media containing 5 mM tyrosine and 0 mM tyrosine and then observed if a color change occurred. The diagram above shows such a color change. Our production assembly did observe a similar color change, but the change in color was not very pronounced.
