Difference between revisions of "Team:CCU Taiwan/Hardware"

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<h1>Hardware</h1>
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<h3>Best Hardware Special Prize</h3>
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<p>iGEM is about making teams of students making synthetic biology projects. We encourage teams to work with parts and build biological devices in the lab. But we are inclusive and want all teams to work on many other types of problems in synbio. Robotic assembly, microfluidics, low cost equipment and measurement hardware are all areas ripe for innovation in synbio. </p>
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Teams who are interested in working with hardware as a side project are encouraged to apply for the hardware award.
 
  
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To compete for the <a href="https://2017.igem.org/Judging/Awards">Best Hardware prize</a>, please describe your work on this page and also fill out the description on the <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>.
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<a href="#Devices">Devices detection</a>
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<li><a href="#light">Light intensity</a></li>
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<li><a href="#pH">pH value</a></li>
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<h2>Hardware overview</h2>
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<p>
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We designed an inexpensive and easy to use fluorescence detection system with Arduino. By simply putting the sample to our container and put it in the device, four kinds of data from the same sample can be detected. What’s more, the inner components are designated to change easily, so that other iGemers can modified it to fit their purpose. <br/>
 +
Usually, bubbles occurred when the sample taken up increase the fluorescence detection error. However, our device took advantage of capillary phenomenon to get the sample to fix this bubble problem. <br/>
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As for the optical system, we use a green filter, a led light, two convex lens and a photodiode. Those thing are inexpensive and successfully detect the fluorescence.
 
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<h5>Inspiration</h5>
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<div class="aaa"></div>
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<p>You can look at what other teams did to get some inspiration! <br />
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<h3>Detecting of the light intensity</h3>
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Here are a few examples:</p>
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<ul>
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<li><a href="https://2016.igem.org/Team:Valencia_UPV">2016 Valencia UPV</a></li>
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<li><a href="https://2016.igem.org/Team:Aachen">2016 Aachen </a></li>
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<li><a href="https://2015.igem.org/Team:TU_Delft">2015 TU Delft  </a></li>
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<li><a href="https://2015.igem.org/Team:TU_Darmstadt">2015 TU Darmstadt</a></li>
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</ul>
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    <p>
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      First, We select the light to frequency converter to receive the light intensity of GFP which is emitted from our CSP and Lactic acid,
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and we collect the frequency from the output of converter for every detection.
 +
Second, we use the measuring instrument in the laboratory to detect accurately to know the light intensity emitted from our CSP and Lactic acid.<br/>
 +
Finally, we model the curve for the light intensity and the frequency, so we can know the light intensity relatively when we collect the frequency from the converter.<br/>
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The following curve is the light intensity of GFP corresponded to the frequency of the converter.<br/><br/>
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<img src="https://static.igem.org/mediawiki/2017/d/d4/F_to_d.png" style="display:block; margin:auto;"><br/>
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    </p>
 
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<div id="pH">
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<h3>Detecting of the pH value</h3>
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We choose the test paper changing its color from pH5.0 to pH8.0, for example, when the color of the test paper changing from red to green, the pH valut changing from 5 to 8,
 +
and then use the color detector to detect the test paper and collect the value of RGB, so we can know the pH value after we draw the curve between the value of RGB and the pH value.<br/>
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<br/>
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<img src="https://static.igem.org/mediawiki/2017/c/ca/Ph_v.png" style="display:block; margin:auto;"><br/>
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Final, these five values (two for the light intensity, and three for the values of Red, Green, and Blue ) we receved will be transmitted from Arduino by our Bluetooth, HC-06, to the APP.
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<div id="Materials">
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<h3>Materials</h3>
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<p>
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Arduino Uno
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Test paper<br/>
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TSL 235R<br/>
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510nm Filter<br/>
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485nm LED<br/>
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TCS3200<br/>
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HC-06<br/>
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Convex lens<br/>
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Small Breadbroad<br/>
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Male to Female DuPont Breadboard Jumper Wires
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Revision as of 18:16, 26 October 2017

No Sidebar - Helios by HTML5 UP

Hardware overview

We designed an inexpensive and easy to use fluorescence detection system with Arduino. By simply putting the sample to our container and put it in the device, four kinds of data from the same sample can be detected. What’s more, the inner components are designated to change easily, so that other iGemers can modified it to fit their purpose.
Usually, bubbles occurred when the sample taken up increase the fluorescence detection error. However, our device took advantage of capillary phenomenon to get the sample to fix this bubble problem.
As for the optical system, we use a green filter, a led light, two convex lens and a photodiode. Those thing are inexpensive and successfully detect the fluorescence.


Detecting of the light intensity

First, We select the light to frequency converter to receive the light intensity of GFP which is emitted from our CSP and Lactic acid, and we collect the frequency from the output of converter for every detection. Second, we use the measuring instrument in the laboratory to detect accurately to know the light intensity emitted from our CSP and Lactic acid.
Finally, we model the curve for the light intensity and the frequency, so we can know the light intensity relatively when we collect the frequency from the converter.
The following curve is the light intensity of GFP corresponded to the frequency of the converter.


Detecting of the pH value

We choose the test paper changing its color from pH5.0 to pH8.0, for example, when the color of the test paper changing from red to green, the pH valut changing from 5 to 8, and then use the color detector to detect the test paper and collect the value of RGB, so we can know the pH value after we draw the curve between the value of RGB and the pH value.


Final, these five values (two for the light intensity, and three for the values of Red, Green, and Blue ) we receved will be transmitted from Arduino by our Bluetooth, HC-06, to the APP.


Materials

Arduino Uno Test paper
TSL 235R
510nm Filter
485nm LED
TCS3200
HC-06
Convex lens
Small Breadbroad
Male to Female DuPont Breadboard Jumper Wires