Hardware overview
We designed an inexpensive and easy to use fluorescence detection system with Arduino. By simply putting the sample to our container and put it in the device, four kinds of data from the same sample can be detected. What’s more, the inner components are designated to change easily, so that other iGemers can modified it to fit their purpose.
Usually, bubbles occurred when the sample taken up increase the fluorescence detection error. However, our device took advantage of capillary phenomenon to get the sample to fix this bubble problem.
As for the optical system, we use a green filter, a led light, two convex lens and a photodiode. Those thing are inexpensive and successfully detect the fluorescence.
Detecting of the light intensity
First, We select the light to frequency converter to receive the light intensity of GFP which is emitted from our CSP and Lactic acid,
and we collect the frequency from the output of converter for every detection.
Second, we use the measuring instrument in the laboratory to detect accurately to know the light intensity emitted from our CSP and Lactic acid.
Finally, we model the curve for the light intensity and the frequency, so we can know the light intensity relatively when we collect the frequency from the converter.
The following curve is the light intensity of GFP corresponded to the frequency of the converter.
Detecting of the pH value
We choose the test paper changing its color from pH5.0 to pH8.0, for example, when the color of the test paper changing from red to green, the pH valut changing from 5 to 8,
and then use the color detector to detect the test paper and collect the value of RGB, so we can know the pH value after we draw the curve between the value of RGB and the pH value.
Final, these five values (two for the light intensity, and three for the values of Red, Green, and Blue ) we receved will be transmitted from Arduino by our Bluetooth, HC-06, to the APP.
Materials
Arduino Uno
Test paper
TSL 235R
510nm Filter
485nm LED
TCS3200
HC-06
Convex lens
Small Breadbroad
Male to Female DuPont Breadboard Jumper Wires
