Description

This year our project is about using light to control the reproduction of E.coli.
We decide to block the replication of E.coli by using dCas9 targeting at OriC.
By splitting dCas9 into two parts, each separately fused with a light inducible dimer.
Under the irradiation of light, the dimer will dimerize, promoting the function of dCas9.
Then the dCas9 will block the OriC, the replication origin of E.coli.
After turning off the light, the dCas9 will split again, which causes the E.coli to reproduce again.