Difference between revisions of "Team:HZAU-China/Description"
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| − | + | <div id="section1" class="jiaozheng"> | |
| − | + | </div> | |
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| − | + | <a class="biaoti">Description</a> | |
| − | + | <a class="zhengwen_disblock" style="margin:0 0 0 30px;">The replication of prokaryotes can be divided into three phases, B C and D</a> | |
| − | + | <a class="yinzhu" href="#yinwen_jiaozheng">$^{[1,2,3]}$</a> | |
| − | + | <a class="zhengwen_disblock">, while there are multi-rounds of replication happened in one cell</a> | |
| − | + | <a class="yinzhu" href="#yinwen_jiaozheng">$^{[1]}$</a> | |
| − | + | <a class="zhengwen_disblock">. So both the phase and the copy number of genome is various in a culture.</a> | |
| − | + | <div> | |
| − | + | <label for="HZAUmenu-toggle" class="zhengwen">To know more about replication | |
| − | + | <span class="caret_black"></span> | |
| − | + | </label> | |
| − | + | <input type="checkbox" id="HZAUmenu-toggle" /> | |
| − | + | <ul id="HZAUmenu"> | |
| − | + | <a style="margin:0 0 0 30px;">The replication process of E. coli can be divided into three phases, phase B, phase C and phase D. B is also called | |
| − | + | pre-replication phase in which cells are preparing for DNA replication, like G1 phase of eukaryotes. C is also | |
| − | + | called replication phase in which the genome is under replication corresponding to phase S in eukaryotic cell cycle. | |
| − | + | The last D phase, of course, is called post-replication phase, in which chromosome separates and one cell divides | |
| − | + | into two corresponding to G2 and M phase in eukaryotes. Among the three phases, C and D are relatively constant, | |
| − | + | about 40 min and 20 min separately, so when to initiate a replication determines the whole cell cycle. Recent research | |
| − | + | reveals a relationship between replication initiation and cell volume, but many details still remain unknown. But | |
| − | + | what we know is that a protein, DnaA, plays an important role in this process. DnaA is a versatile protein possessing | |
| − | + | many different functions related to cell cycle, among which the most important one is to attached to the OriC, | |
| − | + | the origins of chromosome replication, and initiates replication. So controlling cell cycle by interrupting the | |
| − | + | attachment of DnaA and corresponding DNA sequence with dCas9 is an efficient approach. | |
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</a> | </a> | ||
| − | <a | + | <a style="margin:0 0 0 30px;">The whole cell cycle of eukaryotes and prokaryotes show a certain similarity, but there is difference between them. |
| − | + | The cell cycle of prokaryotes can overlap, which means the next round of replication initiates before the last | |
| − | + | replication complete, while eukaryotic cell cycle initiates one after another. Experiments have shown that even | |
| − | + | an isogenetic bacteria grown in the same culture show a difference in both replication phase and genome copy numbers | |
| − | + | and this becomes a huge noise when constructing the 3D genome structure and building up a circuit related to genome | |
| − | + | gene. Besides, the development of synthetic biology requires a system to control the reproduction of engineered | |
| − | + | organisms. So we believe that our project would be a useful tool no matter in theoretical areas or application | |
| − | + | areas.</a> | |
| − | + | </ul> | |
| − | + | </div> | |
| − | + | <img src="https://static.igem.org/mediawiki/2017/4/44/T--HZAU-China--BCDperiod.png" class="tu_1"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2017/1/19/T--HZAU-China--description_Figure2.png" class="tu_2"> | |
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</div> | </div> | ||
</div> | </div> | ||
| + | <a class="zhengwen_disblock" style="margin:0 0 0 30px;">Our problem arises from a research about constructing 4D genome of eukaryote. So we begin to wonder why there isn’t a | ||
| + | 4D genome project of prokaryote. After research we find that due to its complicated replication mechanism, there will | ||
| + | be a huge noise while detecting its structure, which hinder the research on prokaryotic genome | ||
| + | </a> | ||
| + | <a class="yinzhu" href="#yinwen_jiaozheng">$^{[4]}$</a> | ||
| + | <a class="zhengwen_disblock">. </a> | ||
| + | <a class="zhengwen_disblock">Besides, the heterogenicity of cells are gathering importance recently in different fields, like industrial fermentation, | ||
| + | antidrug resistance research and synthetic biology</a> | ||
| + | <a class="yinzhu" href="#yinwen_jiaozheng">$^{[5-7]}$</a> | ||
| + | <a class="zhengwen_disblock">.</a> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/a/a6/T--HZAU-China--computorHand.png" class="tu_3"> | ||
| + | <div> | ||
| + | <div id="section3" class="jiaozheng"> | ||
| + | </div> | ||
| + | </div> | ||
| + | <a class="zhengwen" style="text-indent:30px;">Therefore we begin to think if there could be a methods to eliminate the heterogeneity. While thinking deeper into this | ||
| + | problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon | ||
| + | that can change the traditional statements? | ||
| + | <br>So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time | ||
| + | can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate | ||
| + | than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization | ||
| + | system can also be controlled by machine, by program.</a> | ||
| + | <a class="zhengwen" style="text-indent:30px;">Therefore we begin to think if there could be a method to eliminate the heterogeneity. While thinking deeper into this | ||
| + | problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon | ||
| + | that can change the traditional statements?</a> | ||
| + | <a class="zhengwen" style="text-indent:30px;">So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time | ||
| + | can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate | ||
| + | than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization | ||
| + | system can also be controlled by machine, by program.</a> | ||
| + | <a class="zhengwen" style="text-indent:30px;"> | ||
| + | <br> | ||
| + | </a> | ||
| + | <div> | ||
| + | <div id="yinwen_jiaozheng" class="jiaozheng"> | ||
| + | </div> | ||
| + | </div> | ||
| + | <a class="yinwen">1. Helmstetter CE. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968 | ||
| + | Feb;31(3) 507-518. doi:10.1016/0022-2836(68)90424-5.</a> | ||
| + | <a class="yinwen">2. Skarstad K, Steen HB, Boye E. Cell cycle parameters of slowly growing Escherichia coli B/r studied by flow cytometry. | ||
| + | J Bacteriol. 1983 May;154(2) 656-662.</a> | ||
| + | <a class="yinwen">3. Umbarger, M. A., Toro, E., Wright, M. A., Porreca, G. J., Bau, D., Hong, S. H., . . . Church, G. M. (2011). The three-dimensional | ||
| + | architecture of a bacterial genome and its alteration by genetic perturbation. Mol Cell, 44(2), 252-264. | ||
| + | </a> | ||
| + | <a class="yinwen">4. Paalme, T., Tiisma, K., Kahru, A., Vanatalu, K. & Vilu, R. Glucose-limited fed-batch cultivation of Escherichia coli | ||
| + | with computer-controlled fixed growth rate. Biotechnol. Bioeng. 35, 312–319 (1990).</a> | ||
| + | <a class="yinwen">5. Baumgart, Leo & Mather, William & Hasty, Jeff. (2017). Synchronized DNA cycling across a bacterial population. Nature | ||
| + | Genetics. 49. . 10.1038/ng.3915.</a> | ||
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Revision as of 16:05, 1 November 2017
So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program. Therefore we begin to think if there could be a method to eliminate the heterogeneity. While thinking deeper into this problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon that can change the traditional statements? So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program.
