Difference between revisions of "Team:HZAU-China/Description"
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| − | <a class="zhengwen" style="text-indent:30px;">The replication process of E. coli can be divided into three phases | + | <a class="zhengwen" style="text-indent:30px;">The replication process of E. coli can be divided into three phases; phase B, phase C and phase D. Phase B is also called |
| − | pre-replication phase in which cells are preparing for DNA replication, like G1 phase of eukaryotes. C is also | + | pre-replication phase, in which cells are preparing for DNA replication, like G1 phase of eukaryotes. Phase C is also |
| − | called replication phase in which the genome is under replication corresponding to phase S in eukaryotic cell cycle. | + | called replication phase, in which the genome is under replication, corresponding to phase S in eukaryotic cell cycle. |
| − | The last | + | The last phase D, of course, is called post-replication phase, in which chromosome separates and one cell divides |
| − | into two corresponding to G2 and M | + | into two, corresponding to G2 and M phases in eukaryotes. Among the three phases, C and D are relatively constant, |
| − | about 40 min and 20 min separately, so when to initiate a replication determines the whole cell cycle. Recent | + | about 40 min and 20 min separately, so when to initiate a replication determines the whole cell cycle. Recent work |
| − | + | revealed a relationship between replication initiation and cell volume, but many details still remain unknown. | |
| − | + | What we know is that a protein, DnaA, plays an important role in this process. DnaA is a versatile protein possessing | |
| − | many different functions related to cell cycle, among which the most important one is to | + | many different functions related to cell cycle, among which the most important one is to attach to the OriC, |
the origins of chromosome replication, and initiates replication. So controlling cell cycle by interrupting the | the origins of chromosome replication, and initiates replication. So controlling cell cycle by interrupting the | ||
| − | attachment of DnaA and corresponding DNA sequence with dCas9 is an efficient approach. | + | attachment of DnaA and the corresponding DNA sequence with dCas9 is an efficient approach. |
<br> | <br> | ||
</a> | </a> | ||
Revision as of 18:31, 1 November 2017
Description
The chromosome replication of bacteria can be divided into three phases: B C and D
$^{[1-3]}$
, and meanwhile multi-rounds of replication exist simultaneously in one cell
$^{[1]}$
. So both the replication phase and the copy number of chromosome are heterogeneous in a culture.
Our problem arises from a research about constructing 4D genome of eukaryote. So we begin to wonder why there isn’t a
4D genome project of prokaryote. After research we find that due to its complicated replication mechanism, there will
be a huge noise while detecting its structure, which hinder the research on prokaryotic genome
$^{[4]}$
.
Besides, the heterogenicity of cells are gathering importance recently in different fields, like industrial fermentation,
antidrug resistance research and synthetic biology
$^{[5-7]}$
.
Therefore we begin to think if there could be a methods to eliminate the heterogeneity. While thinking deeper into this
problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon
that can change the traditional statements?
So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program. Therefore we begin to think if there could be a method to eliminate the heterogeneity. While thinking deeper into this problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon that can change the traditional statements? So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program.
1. Helmstetter CE. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968
Feb;31(3) 507-518. doi:10.1016/0022-2836(68)90424-5.
2. Skarstad K, Steen HB, Boye E. Cell cycle parameters of slowly growing Escherichia coli B/r studied by flow cytometry.
J Bacteriol. 1983 May;154(2) 656-662.
3. Umbarger, M. A., Toro, E., Wright, M. A., Porreca, G. J., Bau, D., Hong, S. H., . . . Church, G. M. (2011). The three-dimensional
architecture of a bacterial genome and its alteration by genetic perturbation. Mol Cell, 44(2), 252-264.
4. Paalme, T., Tiisma, K., Kahru, A., Vanatalu, K. & Vilu, R. Glucose-limited fed-batch cultivation of Escherichia coli
with computer-controlled fixed growth rate. Biotechnol. Bioeng. 35, 312–319 (1990).
5. Baumgart, Leo & Mather, William & Hasty, Jeff. (2017). Synchronized DNA cycling across a bacterial population. Nature
Genetics. 49. . 10.1038/ng.3915.
Our problem arises from a research about constructing 4D genome of eukaryote. So we begin to wonder why there isn’t a
4D genome project of prokaryote. After research we find that due to its complicated replication mechanism, there will
be a huge noise while detecting its structure, which hinder the research on prokaryotic genome
$^{[4]}$
.
Besides, the heterogenicity of cells are gathering importance recently in different fields, like industrial fermentation,
antidrug resistance research and synthetic biology
$^{[5-7]}$
.
Therefore we begin to think if there could be a methods to eliminate the heterogeneity. While thinking deeper into this
problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon
that can change the traditional statements?
So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program. Therefore we begin to think if there could be a method to eliminate the heterogeneity. While thinking deeper into this problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon that can change the traditional statements? So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program.
