Difference between revisions of "Team:HZAU-China/Description"
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| − | <a class="zhengwen" style="text-indent:30px;">The whole cell | + | <a class="zhengwen" style="text-indent:30px;">The whole cell cycles of eukaryotes and prokaryotes show a certain similarity, but there is difference between them. |
The cell cycle of prokaryotes can overlap, which means the next round of replication initiates before the last | The cell cycle of prokaryotes can overlap, which means the next round of replication initiates before the last | ||
replication complete, while eukaryotic cell cycle initiates one after another. Experiments have shown that even | replication complete, while eukaryotic cell cycle initiates one after another. Experiments have shown that even | ||
| − | an | + | an isogenic bacteria growing in the same culture show differences in both replication phase and genome copy numbers |
and this becomes a huge noise when constructing the 3D genome structure and building up a circuit related to genome | and this becomes a huge noise when constructing the 3D genome structure and building up a circuit related to genome | ||
| − | + | . Besides, the development of synthetic biology requires a system to control the reproduction of engineered | |
organisms. So we believe that our project would be a useful tool no matter in theoretical areas or application | organisms. So we believe that our project would be a useful tool no matter in theoretical areas or application | ||
areas.</a> | areas.</a> | ||
Revision as of 18:36, 1 November 2017
Description
The chromosome replication of bacteria can be divided into three phases: B C and D
$^{[1-3]}$
, and meanwhile multi-rounds of replication exist simultaneously in one cell
$^{[1]}$
. So both the replication phase and the copy number of chromosome are heterogeneous in a culture.
Our problem arises from a research about constructing 4D genome of eukaryote. So we begin to wonder why there isn’t a
4D genome project of prokaryote. After research we find that due to its complicated replication mechanism, there will
be a huge noise while detecting its structure, which hinder the research on prokaryotic genome
$^{[4]}$
.
Besides, the heterogenicity of cells are gathering importance recently in different fields, like industrial fermentation,
antidrug resistance research and synthetic biology
$^{[5-7]}$
.
Therefore we begin to think if there could be a methods to eliminate the heterogeneity. While thinking deeper into this
problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon
that can change the traditional statements?
So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program. Therefore we begin to think if there could be a method to eliminate the heterogeneity. While thinking deeper into this problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon that can change the traditional statements? So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program.
1. Helmstetter CE. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968
Feb;31(3) 507-518. doi:10.1016/0022-2836(68)90424-5.
2. Skarstad K, Steen HB, Boye E. Cell cycle parameters of slowly growing Escherichia coli B/r studied by flow cytometry.
J Bacteriol. 1983 May;154(2) 656-662.
3. Umbarger, M. A., Toro, E., Wright, M. A., Porreca, G. J., Bau, D., Hong, S. H., . . . Church, G. M. (2011). The three-dimensional
architecture of a bacterial genome and its alteration by genetic perturbation. Mol Cell, 44(2), 252-264.
4. Paalme, T., Tiisma, K., Kahru, A., Vanatalu, K. & Vilu, R. Glucose-limited fed-batch cultivation of Escherichia coli
with computer-controlled fixed growth rate. Biotechnol. Bioeng. 35, 312–319 (1990).
5. Baumgart, Leo & Mather, William & Hasty, Jeff. (2017). Synchronized DNA cycling across a bacterial population. Nature
Genetics. 49. . 10.1038/ng.3915.
Our problem arises from a research about constructing 4D genome of eukaryote. So we begin to wonder why there isn’t a
4D genome project of prokaryote. After research we find that due to its complicated replication mechanism, there will
be a huge noise while detecting its structure, which hinder the research on prokaryotic genome
$^{[4]}$
.
Besides, the heterogenicity of cells are gathering importance recently in different fields, like industrial fermentation,
antidrug resistance research and synthetic biology
$^{[5-7]}$
.
Therefore we begin to think if there could be a methods to eliminate the heterogeneity. While thinking deeper into this
problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon
that can change the traditional statements?
So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program. Therefore we begin to think if there could be a method to eliminate the heterogeneity. While thinking deeper into this problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon that can change the traditional statements? So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program.
