Difference between revisions of "Team:HZAU-China/Description"
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<div class="HZAU_div_main"> | <div class="HZAU_div_main"> | ||
<a class="HZAU_title">Description</a> | <a class="HZAU_title">Description</a> | ||
| − | <a class="zhengwen_disblock" style="margin: | + | <a class="zhengwen_disblock" style="margin-left:30px;">The chromosome replication of bacteria can be divided into three phases: B C and D</a> |
<a class="yinzhu" href="#yinwen_jiaozheng">$^{[1-3]}$</a> | <a class="yinzhu" href="#yinwen_jiaozheng">$^{[1-3]}$</a> | ||
<a class="zhengwen_disblock">, and meanwhile multi-rounds of replication exist simultaneously in one cell</a> | <a class="zhengwen_disblock">, and meanwhile multi-rounds of replication exist simultaneously in one cell</a> | ||
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<input type="checkbox" id="HZAUmenu-toggle" /> | <input type="checkbox" id="HZAUmenu-toggle" /> | ||
<ul id="HZAUmenu"> | <ul id="HZAUmenu"> | ||
| − | <a class="zhengwen | + | <a class="zhengwen">The replication process of E. coli can be divided into three phases; phase B, phase C and phase D. Phase B is also called |
pre-replication phase, in which cells are preparing for DNA replication, like G1 phase of eukaryotes. Phase C is also | pre-replication phase, in which cells are preparing for DNA replication, like G1 phase of eukaryotes. Phase C is also | ||
called replication phase, in which the genome is under replication, corresponding to phase S in eukaryotic cell cycle. | called replication phase, in which the genome is under replication, corresponding to phase S in eukaryotic cell cycle. | ||
| Line 235: | Line 247: | ||
<br> | <br> | ||
</a> | </a> | ||
| − | <a class="zhengwen | + | <a class="zhengwen">The whole cell cycles of eukaryotes and prokaryotes show a certain similarity, but there is difference between them. |
The cell cycle of prokaryotes can overlap, which means the next round of replication initiates before the last | The cell cycle of prokaryotes can overlap, which means the next round of replication initiates before the last | ||
replication complete, while eukaryotic cell cycle initiates one after another. Experiments have shown that even | replication complete, while eukaryotic cell cycle initiates one after another. Experiments have shown that even | ||
| Line 247: | Line 259: | ||
<img src="https://static.igem.org/mediawiki/2017/4/44/T--HZAU-China--BCDperiod.png" class="tu_1"> | <img src="https://static.igem.org/mediawiki/2017/4/44/T--HZAU-China--BCDperiod.png" class="tu_1"> | ||
<img src="https://static.igem.org/mediawiki/2017/1/19/T--HZAU-China--description_Figure2.png" class="tu_2"> | <img src="https://static.igem.org/mediawiki/2017/1/19/T--HZAU-China--description_Figure2.png" class="tu_2"> | ||
| − | <a class="zhengwen_disblock" style="margin: | + | <a class="zhengwen_disblock" style="margin-left:30px;">Our problem arises from a research about constructing 4D genome of eukaryote. So we begin to wonder why there isn’t a |
4D genome project of prokaryote. After research we find that due to its complicated replication mechanism, there will | 4D genome project of prokaryote. After research we find that due to its complicated replication mechanism, there will | ||
be a huge noise while detecting its structure, which hinder the research on prokaryotic genome | be a huge noise while detecting its structure, which hinder the research on prokaryotic genome | ||
| Line 253: | Line 265: | ||
<a class="yinzhu" href="#yinwen_jiaozheng">$^{[4]}$</a> | <a class="yinzhu" href="#yinwen_jiaozheng">$^{[4]}$</a> | ||
<a class="zhengwen_disblock">. </a> | <a class="zhengwen_disblock">. </a> | ||
| − | <a class="zhengwen_disblock">Besides, the heterogenicity of cells are gathering importance recently in different fields, like industrial fermentation, | + | <a class="zhengwen_disblock" style="margin-left:30px;">Besides, the heterogenicity of cells are gathering importance recently in different fields, like industrial fermentation, |
antidrug resistance research and synthetic biology</a> | antidrug resistance research and synthetic biology</a> | ||
<a class="yinzhu" href="#yinwen_jiaozheng">$^{[5-7]}$</a> | <a class="yinzhu" href="#yinwen_jiaozheng">$^{[5-7]}$</a> | ||
<a class="zhengwen_disblock">.</a> | <a class="zhengwen_disblock">.</a> | ||
<img src="https://static.igem.org/mediawiki/2017/a/a6/T--HZAU-China--computorHand.png" class="tu_3"> | <img src="https://static.igem.org/mediawiki/2017/a/a6/T--HZAU-China--computorHand.png" class="tu_3"> | ||
| − | <a class="zhengwen | + | <a class="zhengwen">Therefore we begin to think if there could be a methods to eliminate the heterogeneity. While thinking deeper into this |
problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon | problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon | ||
that can change the traditional statements? | that can change the traditional statements? | ||
| Line 265: | Line 277: | ||
than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization | than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization | ||
system can also be controlled by machine, by program.</a> | system can also be controlled by machine, by program.</a> | ||
| − | <a class="zhengwen | + | <a class="zhengwen">Therefore we begin to think if there could be a method to eliminate the heterogeneity. While thinking deeper into this |
problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon | problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon | ||
that can change the traditional statements?</a> | that can change the traditional statements?</a> | ||
| − | <a class="zhengwen | + | <a class="zhengwen">So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time |
can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate | can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate | ||
than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization | than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization | ||
system can also be controlled by machine, by program.</a> | system can also be controlled by machine, by program.</a> | ||
| − | <a class="zhengwen | + | <a class="zhengwen"> |
<br> | <br> | ||
</a> | </a> | ||
Revision as of 22:38, 1 November 2017
Description
The chromosome replication of bacteria can be divided into three phases: B C and D
$^{[1-3]}$
, and meanwhile multi-rounds of replication exist simultaneously in one cell
$^{[1]}$
. So both the replication phase and the copy number of chromosome are heterogeneous in a culture.
Our problem arises from a research about constructing 4D genome of eukaryote. So we begin to wonder why there isn’t a
4D genome project of prokaryote. After research we find that due to its complicated replication mechanism, there will
be a huge noise while detecting its structure, which hinder the research on prokaryotic genome
$^{[4]}$
.
Besides, the heterogenicity of cells are gathering importance recently in different fields, like industrial fermentation,
antidrug resistance research and synthetic biology
$^{[5-7]}$
.
Therefore we begin to think if there could be a methods to eliminate the heterogeneity. While thinking deeper into this
problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon
that can change the traditional statements?
So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program. Therefore we begin to think if there could be a method to eliminate the heterogeneity. While thinking deeper into this problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon that can change the traditional statements? So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program.
References
1. Helmstetter CE. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968
Feb;31(3) 507-518. doi:10.1016/0022-2836(68)90424-5.
2. Skarstad K, Steen HB, Boye E. Cell cycle parameters of slowly growing Escherichia coli B/r studied by flow cytometry.
J Bacteriol. 1983 May;154(2) 656-662.
3. Umbarger, M. A., Toro, E., Wright, M. A., Porreca, G. J., Bau, D., Hong, S. H., . . . Church, G. M. (2011). The three-dimensional
architecture of a bacterial genome and its alteration by genetic perturbation. Mol Cell, 44(2), 252-264.
4. Paalme, T., Tiisma, K., Kahru, A., Vanatalu, K. & Vilu, R. Glucose-limited fed-batch cultivation of Escherichia coli
with computer-controlled fixed growth rate. Biotechnol. Bioeng. 35, 312–319 (1990).
5. Baumgart, Leo & Mather, William & Hasty, Jeff. (2017). Synchronized DNA cycling across a bacterial population. Nature
Genetics. 49. . 10.1038/ng.3915.
Our problem arises from a research about constructing 4D genome of eukaryote. So we begin to wonder why there isn’t a
4D genome project of prokaryote. After research we find that due to its complicated replication mechanism, there will
be a huge noise while detecting its structure, which hinder the research on prokaryotic genome
$^{[4]}$
.
Besides, the heterogenicity of cells are gathering importance recently in different fields, like industrial fermentation,
antidrug resistance research and synthetic biology
$^{[5-7]}$
.
Therefore we begin to think if there could be a methods to eliminate the heterogeneity. While thinking deeper into this
problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon
that can change the traditional statements?
So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program. Therefore we begin to think if there could be a method to eliminate the heterogeneity. While thinking deeper into this problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon that can change the traditional statements? So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program.
