Difference between revisions of "Team:HZAU-China/Description"
| Line 1: | Line 1: | ||
{{HZAU-China}} | {{HZAU-China}} | ||
<html> | <html> | ||
| + | |||
<head> | <head> | ||
<meta charset="utf-8"> | <meta charset="utf-8"> | ||
| Line 91: | Line 92: | ||
/*改变超链接的位置*/ | /*改变超链接的位置*/ | ||
| − | .jiaozheng{ | + | |
| + | .jiaozheng { | ||
position: relative; | position: relative; | ||
top: -66px; | top: -66px; | ||
| Line 137: | Line 139: | ||
color: rgb(63, 59, 58); | color: rgb(63, 59, 58); | ||
} | } | ||
| − | + | ||
.zhengwen { | .zhengwen { | ||
display: block; | display: block; | ||
| Line 163: | Line 165: | ||
} | } | ||
| − | .yinzhu{ | + | .yinzhu {} |
| − | + | ||
| − | + | ||
| − | .yinzhu:hover .yinzhu{ | + | .yinzhu:hover .yinzhu {} |
| − | + | ||
| − | + | ||
| − | .yinwen{ | + | .yinwen { |
display: block; | display: block; | ||
font-style: arial; | font-style: arial; | ||
| Line 183: | Line 181: | ||
margin-top: 7.2px; | margin-top: 7.2px; | ||
} | } | ||
| + | |||
.shangduiqi { | .shangduiqi { | ||
position: relative; | position: relative; | ||
| Line 193: | Line 192: | ||
/*图片的样式*/ | /*图片的样式*/ | ||
| − | + | .tu_1 { | |
| − | . | + | width: 600px; |
margin: 0 auto; | margin: 0 auto; | ||
display: block; | display: block; | ||
} | } | ||
| − | . | + | .tu_2 { |
| − | width: | + | width: 800px; |
| + | margin: 0 auto; | ||
| + | display: block; | ||
} | } | ||
| − | . | + | .tu_3 { |
width: 555px; | width: 555px; | ||
| + | margin: 0 auto; | ||
| + | display: block; | ||
} | } | ||
</style> | </style> | ||
| Line 230: | Line 233: | ||
#HZAUmenu { | #HZAUmenu { | ||
display: none; | display: none; | ||
| − | font-size: | + | font-size: 18px; |
padding: 0 50px 0 50px; | padding: 0 50px 0 50px; | ||
| − | width: | + | width: 900px; |
margin: 0; | margin: 0; | ||
color: black; | color: black; | ||
| Line 284: | Line 287: | ||
</div> | </div> | ||
<a class="biaoti">Description</a> | <a class="biaoti">Description</a> | ||
| − | <a class="zhengwen_disblock">The replication of prokaryotes can be divided into three phases, B C and D</a><a class="yinzhu" href="#yinwen_jiaozheng">$^{[1,2,3]}$</a><a class="zhengwen_disblock">, while there are multi-rounds of replication happened in one cell</a><a class="yinzhu" href="#yinwen_jiaozheng">$^{[1]}$</a><a class="zhengwen_disblock">. So both the phase and the copy number of genome is various in a culture.</a> | + | <a class="zhengwen_disblock" style="margin:0 0 0 30px;">The replication of prokaryotes can be divided into three phases, B C and D</a> |
| + | <a class="yinzhu" href="#yinwen_jiaozheng">$^{[1,2,3]}$</a> | ||
| + | <a class="zhengwen_disblock">, while there are multi-rounds of replication happened in one cell</a> | ||
| + | <a class="yinzhu" href="#yinwen_jiaozheng">$^{[1]}$</a> | ||
| + | <a class="zhengwen_disblock">. So both the phase and the copy number of genome is various in a culture.</a> | ||
<div> | <div> | ||
<label for="HZAUmenu-toggle" class="zhengwen">To know more about replication | <label for="HZAUmenu-toggle" class="zhengwen">To know more about replication | ||
| Line 291: | Line 298: | ||
<input type="checkbox" id="HZAUmenu-toggle" /> | <input type="checkbox" id="HZAUmenu-toggle" /> | ||
<ul id="HZAUmenu"> | <ul id="HZAUmenu"> | ||
| − | <a>The replication process of E. coli can be divided into three phases, phase B, phase C and phase D. B is also | + | <a style="margin:0 0 0 30px;">The replication process of E. coli can be divided into three phases, phase B, phase C and phase D. B is also |
called pre-replication phase in which cells are preparing for DNA replication, like G1 phase of eukaryotes. | called pre-replication phase in which cells are preparing for DNA replication, like G1 phase of eukaryotes. | ||
C is also called replication phase in which the genome is under replication corresponding to phase S in eukaryotic | C is also called replication phase in which the genome is under replication corresponding to phase S in eukaryotic | ||
| Line 303: | Line 310: | ||
So controlling cell cycle by interrupting the attachment of DnaA and corresponding DNA sequence with dCas9 | So controlling cell cycle by interrupting the attachment of DnaA and corresponding DNA sequence with dCas9 | ||
is an efficient approach. | is an efficient approach. | ||
| − | <br>The whole cell cycle of eukaryotes and prokaryotes show a certain similarity, but there is difference between | + | <br></a> |
| + | <a style="margin:0 0 0 30px;">The whole cell cycle of eukaryotes and prokaryotes show a certain similarity, but there is difference between | ||
them. The cell cycle of prokaryotes can overlap, which means the next round of replication initiates before | them. The cell cycle of prokaryotes can overlap, which means the next round of replication initiates before | ||
the last replication complete, while eukaryotic cell cycle initiates one after another. Experiments have shown | the last replication complete, while eukaryotic cell cycle initiates one after another. Experiments have shown | ||
| Line 314: | Line 322: | ||
</div> | </div> | ||
<img src="https://static.igem.org/mediawiki/2017/4/44/T--HZAU-China--BCDperiod.png" class="tu_1"> | <img src="https://static.igem.org/mediawiki/2017/4/44/T--HZAU-China--BCDperiod.png" class="tu_1"> | ||
| − | <img src="https://static.igem.org/mediawiki/2017/1/19/T--HZAU-China--description_Figure2.png" class=" | + | <img src="https://static.igem.org/mediawiki/2017/1/19/T--HZAU-China--description_Figure2.png" class="tu_2"> |
<div> | <div> | ||
<div id="section2" class="jiaozheng"> | <div id="section2" class="jiaozheng"> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <a class="zhengwen_disblock">Our problem arises from a research about constructing 4D genome of eukaryote. So we begin to wonder why there isn’t | + | <a class="zhengwen_disblock" style="margin:0 0 0 30px;">Our problem arises from a research about constructing 4D genome of eukaryote. So we begin to wonder why there isn’t |
a 4D genome project of prokaryote. After research we find that due to its complicated replication mechanism, there | a 4D genome project of prokaryote. After research we find that due to its complicated replication mechanism, there | ||
will be a huge noise while detecting its structure, which hinder the research on prokaryotic genome | will be a huge noise while detecting its structure, which hinder the research on prokaryotic genome | ||
| − | + | </a> | |
| + | <a class="yinzhu" href="#yinwen_jiaozheng">$^{[4]}$</a> | ||
| + | <a class="zhengwen_disblock">. </a> | ||
<a class="zhengwen_disblock">Besides, the heterogenicity of cells are gathering importance recently in different fields, like industrial fermentation, | <a class="zhengwen_disblock">Besides, the heterogenicity of cells are gathering importance recently in different fields, like industrial fermentation, | ||
| − | antidrug resistance research and synthetic biology</a><a class="yinzhu" href="#yinwen_jiaozheng">$^{[5-7]}$</a><a class="zhengwen_disblock">.</a> | + | antidrug resistance research and synthetic biology</a> |
| − | <img src="https://static.igem.org/mediawiki/2017/a/a6/T--HZAU-China--computorHand.png" class=" | + | <a class="yinzhu" href="#yinwen_jiaozheng">$^{[5-7]}$</a> |
| + | <a class="zhengwen_disblock">.</a> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/a/a6/T--HZAU-China--computorHand.png" class="tu_3"> | ||
<div> | <div> | ||
<div id="section3" class="jiaozheng"> | <div id="section3" class="jiaozheng"> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <a class="zhengwen">Therefore we begin to think if there could be a methods to eliminate the heterogeneity. While thinking deeper into | + | <a class="zhengwen" style="text-indent:30px;">Therefore we begin to think if there could be a methods to eliminate the heterogeneity. While thinking deeper into |
this problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a | this problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a | ||
new phenomenon that can change the traditional statements? | new phenomenon that can change the traditional statements? | ||
| Line 337: | Line 349: | ||
accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our | accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our | ||
synchronization system can also be controlled by machine, by program.</a> | synchronization system can also be controlled by machine, by program.</a> | ||
| − | <a class="zhengwen">Therefore we begin to think if there could be a method to eliminate the heterogeneity. While thinking deeper into | + | <a class="zhengwen" style="text-indent:30px;">Therefore we begin to think if there could be a method to eliminate the heterogeneity. While thinking deeper into |
this problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a | this problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a | ||
new phenomenon that can change the traditional statements?</a> | new phenomenon that can change the traditional statements?</a> | ||
| − | <a class="zhengwen">So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same | + | <a class="zhengwen" style="text-indent:30px;">So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same |
time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more | time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more | ||
accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our | accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our | ||
synchronization system can also be controlled by machine, by program.</a> | synchronization system can also be controlled by machine, by program.</a> | ||
| − | <div> | + | <a class="zhengwen" style="text-indent:30px;"> <br></a> |
| − | + | <div> | |
| − | + | <div id="yinwen_jiaozheng" class="jiaozheng"> | |
| − | + | </div> | |
| + | </div> | ||
<a class="yinwen">1. Helmstetter CE. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968 | <a class="yinwen">1. Helmstetter CE. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968 | ||
Feb;31(3) 507-518. doi:10.1016/0022-2836(68)90424-5.</a> | Feb;31(3) 507-518. doi:10.1016/0022-2836(68)90424-5.</a> | ||
<a class="yinwen">2. Skarstad K, Steen HB, Boye E. Cell cycle parameters of slowly growing Escherichia coli B/r studied by flow cytometry. | <a class="yinwen">2. Skarstad K, Steen HB, Boye E. Cell cycle parameters of slowly growing Escherichia coli B/r studied by flow cytometry. | ||
J Bacteriol. 1983 May;154(2) 656-662.</a> | J Bacteriol. 1983 May;154(2) 656-662.</a> | ||
| − | <a class="yinwen">3. Umbarger, M. A., Toro, E., Wright, M. A., Porreca, G. J., Bau, D., Hong, S. H., . . . Church, G. M. (2011). The three-dimensional | + | <a class="yinwen">3. Umbarger, M. A., Toro, E., Wright, M. A., Porreca, G. J., Bau, D., Hong, S. H., . . . Church, G. M. (2011). The |
| − | + | three-dimensional architecture of a bacterial genome and its alteration by genetic perturbation. Mol Cell, 44(2), | |
| − | <a class="yinwen">4. Paalme, T., Tiisma, K., Kahru, A., Vanatalu, K. & Vilu, R. Glucose-limited fed-batch cultivation of Escherichia | + | 252-264.</a> |
| − | with computer-controlled fixed growth rate. Biotechnol. Bioeng. 35, 312–319 (1990).</a> | + | <a class="yinwen">4. Paalme, T., Tiisma, K., Kahru, A., Vanatalu, K. & Vilu, R. Glucose-limited fed-batch cultivation of Escherichia |
| − | <a class="yinwen">5. Baumgart, Leo & Mather, William & Hasty, Jeff. (2017). Synchronized DNA cycling across a bacterial population. | + | coli with computer-controlled fixed growth rate. Biotechnol. Bioeng. 35, 312–319 (1990).</a> |
| − | Genetics. 49. . 10.1038/ng.3915.</a> | + | <a class="yinwen">5. Baumgart, Leo & Mather, William & Hasty, Jeff. (2017). Synchronized DNA cycling across a bacterial population. |
| + | Nature Genetics. 49. . 10.1038/ng.3915.</a> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 00:04, 1 November 2017
So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program. Therefore we begin to think if there could be a method to eliminate the heterogeneity. While thinking deeper into this problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new phenomenon that can change the traditional statements? So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program.
