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<a class="zhengwen">Our whole plan is to control the cell replication dynamically. After research, we decided to combine the CRISPR and optogenetic to construct our system.</a> | <a class="zhengwen">Our whole plan is to control the cell replication dynamically. After research, we decided to combine the CRISPR and optogenetic to construct our system.</a> | ||
<img src="https://i0.hdslb.com/bfs/bangumi/71ff7c74c34eeac1c12379c40126d200233780dd.jpg_144x144.jpg" class="tu_1"> | <img src="https://i0.hdslb.com/bfs/bangumi/71ff7c74c34eeac1c12379c40126d200233780dd.jpg_144x144.jpg" class="tu_1"> | ||
− | <a class="zhengwen_disblock">Like mentioned in description, DnaA protein is essential for cell cycle control</a><a class="yinzhu" href="#yinwen_jiaozheng">$^{[1]}$</a><a class="zhengwen_disblock">, so we decide to interrupt its binding to corresponding DNA using CRISPR/dCas9. Under the guidance of gRNA dCas9 will bind to where DnaA should bind blocking further reaction to form replisome</a><a class="yinzhu" href="#yinwen_jiaozheng">$^{[ | + | <a class="zhengwen_disblock">Like mentioned in description, DnaA protein is essential for cell cycle control</a><a class="yinzhu" href="#yinwen_jiaozheng">$^{[1]}$</a><a class="zhengwen_disblock">, so we decide to interrupt its binding to corresponding DNA using CRISPR/dCas9. Under the guidance of gRNA dCas9 will bind to where DnaA should bind blocking further reaction to form replisome</a><a class="yinzhu" href="#yinwen_jiaozheng">$^{[2]}$</a><a class="zhengwen_disblock">. Without replisome, the chromosome can’t replicate block it at phase B. Compared with engineering DnaA protein, this method is more easy and robust, and can be readily integrated into other prokaryotes.</a> |
<img src="https://i0.hdslb.com/bfs/bangumi/71ff7c74c34eeac1c12379c40126d200233780dd.jpg_144x144.jpg" class="tu_1"> | <img src="https://i0.hdslb.com/bfs/bangumi/71ff7c74c34eeac1c12379c40126d200233780dd.jpg_144x144.jpg" class="tu_1"> | ||
<a class="zhengwen">Optogenetic is used to regulate the function of dCas9 due to its specificity in time and space and its easy manipulation, and it is also considered to be a proper connection between organism and computer. So we decided to use light to control the whole system. To accomplish this system, we designed two approaches, one is based on the reaction of transcription level, and the other is based on the protein interaction.</a> | <a class="zhengwen">Optogenetic is used to regulate the function of dCas9 due to its specificity in time and space and its easy manipulation, and it is also considered to be a proper connection between organism and computer. So we decided to use light to control the whole system. To accomplish this system, we designed two approaches, one is based on the reaction of transcription level, and the other is based on the protein interaction.</a> |
Revision as of 02:41, 1 November 2017