Difference between revisions of "Team:HZAU-China/Improve"

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<a class="biaoti">Improve</a>
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This year we improved the CcaS-CcaR system used in last year. By changing the RBS strength of CcaR, we optimized the expression of sfGFP from 2-fold variance up to 3-fold variance
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<a class="fubiaoti">Optimized CcaS-CcaR system</a>
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<a class="zhengwen">This year we improved the CcaS-CcaR system used in last year. By changing the RBS strength of CcaR, we optimized the expression of sfGFP from 2-fold variance up to 3-fold variance. </a>
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<img src="https://static.igem.org/mediawiki/2017/6/63/T--HZAU-China--sRBS.png">
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<img src="https://static.igem.org/mediawiki/2017/3/36/T--HZAU-China--improve.png">
  
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<a class="zhengwen">A further improvement is conducted by knocking the PcpcG2-sfGFP into the genome of E.coli. A significant enhancement of this system is observed.</a>
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<img src="https://static.igem.org/mediawiki/2017/7/79/T--HZAU-China--improvement.png">
  
 
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Revision as of 03:09, 2 November 2017

Improve Optimized CcaS-CcaR system This year we improved the CcaS-CcaR system used in last year. By changing the RBS strength of CcaR, we optimized the expression of sfGFP from 2-fold variance up to 3-fold variance. A further improvement is conducted by knocking the PcpcG2-sfGFP into the genome of E.coli. A significant enhancement of this system is observed.