Difference between revisions of "Team:HZAU-China/Improve"

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<a class="biaoti">Improve</a>
 
<a class="biaoti">Improve</a>
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<a class="fubiaoti">Optimized CcaS-CcaR system</a>
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<a class="fubiaoti">Optimized CcaS-CcaR system <a href="http://parts.igem.org/Part:BBa_K2012023">BBa_K2012023</a></a>
 
<a class="zhengwen">This year we improved the CcaS-CcaR system used in last year. By changing the RBS strength of CcaR, we optimized the expression of sfGFP from 2-fold variance up to 3-fold variance. </a>  
 
<a class="zhengwen">This year we improved the CcaS-CcaR system used in last year. By changing the RBS strength of CcaR, we optimized the expression of sfGFP from 2-fold variance up to 3-fold variance. </a>  
 
<img src="https://static.igem.org/mediawiki/2017/6/63/T--HZAU-China--sRBS.png" width="25%">
 
<img src="https://static.igem.org/mediawiki/2017/6/63/T--HZAU-China--sRBS.png" width="25%">

Revision as of 03:14, 2 November 2017

Improve Optimized CcaS-CcaR system BBa_K2012023 This year we improved the CcaS-CcaR system used in last year. By changing the RBS strength of CcaR, we optimized the expression of sfGFP from 2-fold variance up to 3-fold variance. A further improvement is conducted by knocking the PcpcG2-sfGFP into the genome of E.coli. A significant enhancement of this system is observed.