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− | <!DOCTYPE html>
| + | {{HZAU-China}} |
− | <html lang="en"> | + | <html> |
| | | |
| <head> | | <head> |
− | <meta charset="UTF-8"> | + | <meta charset="utf-8"> |
− | <!--一些初始设置--> | + | <meta name="viewport" content="width=device-width, initial-scale=1"> |
− | <style type="text/css"> | + | <link rel="stylesheet" href="http://cdn.static.runoob.com/libs/bootstrap/3.3.7/css/bootstrap.min.css"> |
− | /* HTML5 display-role reset for older browsers */ | + | <script src="http://cdn.static.runoob.com/libs/jquery/2.1.1/jquery.min.js"></script> |
| + | <script src="http://cdn.static.runoob.com/libs/bootstrap/3.3.7/js/bootstrap.min.js"></script> |
| + | <style> |
| + | /*小导航栏样式*/ |
| | | |
− | article, | + | ul.nav-pills { |
− | aside,
| + | background: url(https://static.igem.org/mediawiki/2017/3/35/T--HZAU-China--background.png); |
− | details,
| + | background-size: 100% auto; |
− | figcaption,
| + | background-repeat: no-repeat; |
− | figure,
| + | |
− | footer,
| + | |
− | header,
| + | |
− | hgroup,
| + | |
− | nav,
| + | |
− | section {
| + | |
− | display: block;
| + | |
− | }
| + | |
− | | + | |
− | ol,
| + | |
− | ul {
| + | |
− | list-style: none;
| + | |
− | }
| + | |
− | | + | |
− | blockquote,
| + | |
− | q {
| + | |
− | quotes: none; | + | |
− | }
| + | |
− | | + | |
− | blockquote:before,
| + | |
− | blockquote:after,
| + | |
− | q:before,
| + | |
− | q:after {
| + | |
− | content: '';
| + | |
− | content: none; | + | |
− | }
| + | |
− | | + | |
− | table {
| + | |
− | border-collapse: collapse; | + | |
− | border-spacing: 0;
| + | |
− | }
| + | |
− | </style>
| + | |
− | <!-- HZAU-navigation's style -->
| + | |
− | <style type="text/css">
| + | |
− | div.nav_body {
| + | |
| position: fixed; | | position: fixed; |
− | padding: 0px;
| |
− | left: 0px;
| |
− | top: 16px;
| |
− | width: 100%;
| |
− | min-width: 950px;
| |
− | height: 36px;
| |
− | box-shadow: 0px 1px 3px #aaaaaa;
| |
− | z-index: 1;
| |
− | color: rgb(249, 228, 137);
| |
− | text-decoration-line: none;
| |
− | font-family: "Arial", Helvetica, sans-serif;
| |
− | background-color: #000;
| |
− | z-index: 99;
| |
− | }
| |
− |
| |
− | ul#nav_organ {
| |
− | float: right;
| |
− | list-style: none;
| |
− | margin: 6px auto;
| |
− | text-decoration-line: none;
| |
− | font-family: "Arial", Helvetica, sans-serif;
| |
− | color: rgb(249, 228, 137);
| |
− | background-color: #000;
| |
− | }
| |
− |
| |
− | ul#nav_organ li {
| |
| display: block; | | display: block; |
− | float: left; | + | top: 50%; |
| + | margin-top: -144.5px; |
| + | height: 289px; |
| + | width: 200px; |
| + | overflow: hidden; |
| } | | } |
| | | |
− | a.nav_tissue { | + | ul.nav-pills>li { |
− | padding: 8px 15px 8px 15px; | + | position: relative; |
− | line-height: 20px; | + | top: 50%; |
− | text-decoration-line: none;
| + | |
− | font-family: "Arial", Helvetica, sans-serif;
| + | |
− | color: rgb(249, 228, 137);
| + | |
− | font-size: 16px;
| + | |
| } | | } |
| | | |
− | span.caret { | + | #change_top { |
− | display: inline-block;
| + | margin-top: -60px; |
− | margin-left: 2px; | + | |
− | vertical-align: middle;
| + | |
− | width: 0;
| + | |
− | height: 0;
| + | |
− | border-left: 4px solid transparent;
| + | |
− | border-right: 4px solid transparent;
| + | |
− | border-top: 4px solid rgb(249, 228, 137);
| + | |
| } | | } |
| | | |
− | .nav_cell { | + | /*小导航栏触碰后变色*/ |
− | visibility: hidden;
| + | |
− | opacity: 0;
| + | |
− | display: block;
| + | |
− | box-sizing: border-box;
| + | |
− | left: auto;
| + | |
− | position: absolute;
| + | |
− | top: 30px;
| + | |
− | z-index: 1000;
| + | |
− | float: left;
| + | |
− | min-width: 160px;
| + | |
− | margin: 0 0 0 -4px;
| + | |
− | font-size: 14px;
| + | |
− | text-align: left;
| + | |
− | list-style: none;
| + | |
− | color: rgb(249, 228, 137);
| + | |
− | background-color: #000;
| + | |
− | background-clip: padding-box;
| + | |
− | border: 1px solid rgba(0, 0, 0, .15);
| + | |
− | border-radius: 4px;
| + | |
− | box-shadow: 0 6px 12px rgba(0, 0, 0, .175);
| + | |
− | -webkit-transition: opacity 0.4s linear;
| + | |
− | -moz-transition: opacity 0.4s linear;
| + | |
− | -ms-transition: opacity 0.4s linear;
| + | |
− | -o-transition: opacity 0.4s linear;
| + | |
− | transition: opacity 0.4s linear;
| + | |
− | }
| + | |
| | | |
− | .mw-content-ltr ul, | + | /*改变超链接的位置*/ |
− | .mw-content-rtl .mw-content-ltr ul {
| + | |
− | margin: 0 0 0 -4px;
| + | |
− | }
| + | |
| | | |
− | .shadow { | + | #section1 { |
− | box-shadow: 0px 1px 3px rgb(249, 228, 137); | + | position: relative; |
| + | top: -66px; |
| } | | } |
| | | |
− | .nav_cell>li { | + | #section2 { |
− | display: block; | + | position: relative; |
− | float: left; | + | top: -66px; |
− | clear: both;
| + | |
− | height: 30px;
| + | |
| } | | } |
| | | |
− | .nav_cell>li>a {
| + | #section3 { |
− | padding-top: 3px;
| + | position: relative; |
− | padding-bottom: 3px;
| + | top: -66px; |
− | padding-left: 20px;
| + | |
− | padding-right: 20px;
| + | |
− | text-decoration-line: none;
| + | |
− | color: rgb(249, 228, 137);
| + | |
− | }
| + | |
− | | + | |
− | .menu_item:hover .nav_cell {
| + | |
− | visibility: visible;
| + | |
− | opacity: 1;
| + | |
− | }
| + | |
− | </style>
| + | |
− | <!--消除一些不兼容引起的错拍-->
| + | |
− | <style type="text/css">
| + | |
− | .ppp {
| + | |
− | display: block;
| + | |
− | padding: 100px;
| + | |
− | }
| + | |
− | | + | |
− | #sideMenu,
| + | |
− | #top_title,
| + | |
− | #team_submenu { | + | |
− | display: none; | + | |
− | }
| + | |
− | | + | |
− | #content {
| + | |
− | padding: 0px; | + | |
− | width: 100%;
| + | |
− | margin-top: 0px;
| + | |
− | margin-left: 0px;
| + | |
− | margin: 0 0 0 0;
| + | |
− | }
| + | |
− | | + | |
− | #bodyContent h1,
| + | |
− | #bodyContent h2,
| + | |
− | #bodyContent h3,
| + | |
− | #bodyContent h4,
| + | |
− | #bodyContent h5 {
| + | |
− | margin-bottom: 0px;
| + | |
− | }
| + | |
− | | + | |
− | #team_name {
| + | |
− | display: none
| + | |
− | }
| + | |
− | | + | |
− | .global_wrapper {
| + | |
− | padding: 0px 0px 0px 0px;
| + | |
− | }
| + | |
− | | + | |
− | #top_menu_14 {
| + | |
− | height: 16px;
| + | |
| } | | } |
| </style> | | </style> |
| <!--内容的样式--> | | <!--内容的样式--> |
| <style> | | <style> |
| + | /*总样式*/ |
| + | |
| body { | | body { |
| font-family: "Arial", Helvetica, sans-serif; | | font-family: "Arial", Helvetica, sans-serif; |
− | }
| + | text-align: justify; |
− | | + | margin: 0px; |
− | .HZAU_content {
| + | background-color: #ffffff; |
− | position: relative; | + | |
− | right: 0px; | + | |
− | width: 1000px; | + | |
− | height: 3000px;
| + | |
− | margin: 0 0 0 250px;
| + | |
− | z-index: -1;
| + | |
− | }
| + | |
− | | + | |
− | .HZAU_2017_menu {
| + | |
− | display: block;
| + | |
− | position: fixed;
| + | |
− | left: 15px;
| + | |
− | top: 100px;
| + | |
− | height: 337px;
| + | |
− | width: 233px;
| + | |
− | overflow: hidden;
| + | |
− | }
| + | |
− | | + | |
− | .beijin {
| + | |
− | height: 337px;
| + | |
− | width: 233px;
| + | |
| } | | } |
| | | |
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| .HZAU_2017_menu li { | | .HZAU_2017_menu li { |
| width: 100%; | | width: 100%; |
| + | line-height: 0em; |
| + | list-style-image: none; |
| } | | } |
| | | |
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| } | | } |
| | | |
− | .item { | + | /*内容框子的样式*/ |
− | width: 100%;
| + | |
− | padding-left: 40px;
| + | |
| | | |
| + | .col-sm-9 { |
| + | position: absolute; |
| + | top: 43px; |
| + | left: 50%; |
| + | margin-left: -330px; |
| + | width: 900px; |
| + | height: 2300px; |
| + | z-index: -1; |
| + | } |
| | | |
| + | /*内容中几种文体的样式*/ |
| + | |
| + | .col-sm-9>a:hover { |
| + | color: #000; |
| + | text-decoration: none; |
| } | | } |
| | | |
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| | | |
| .zhengwen { | | .zhengwen { |
| + | display: block; |
| font-style: arial; | | font-style: arial; |
− | font-size: 24px; | + | font-size: 18px; |
− | line-height: 120%; | + | line-height: 34px; |
| text-align: justify; | | text-align: justify; |
| color: #000; | | color: #000; |
| + | margin-bottom: 9px; |
| + | margin-left: 0px; |
| + | margin-right: 0px; |
| + | margin-top: 7.2px |
| } | | } |
| | | |
− | .tu_1 { | + | .shangduiqi { |
| position: relative; | | position: relative; |
− | left: 450px; | + | } |
− | padding-top: 30px; | + | |
− | padding-bottom: 30px;
| + | .shangduiqi a { |
| + | position: absolute; |
| + | top: -66px; |
| + | } |
| + | |
| + | /*图片的样式*/ |
| + | |
| + | .col-sm-9 img { |
| + | margin: 0 auto; |
| + | display: block; |
| + | } |
| + | |
| + | .tu_1 { |
| + | width: 666px; |
| } | | } |
| | | |
| .tu_2 { | | .tu_2 { |
− | position: relative; | + | width: 555px; |
− | left: 450px; | + | } |
− | padding-top: 30px; | + | </style> |
− | padding-bottom: 30px; | + | <!--点击展开部分的样式--> |
| + | <style> |
| + | span.caret_black { |
| + | display: inline-block; |
| + | margin-left: 6px; |
| + | vertical-align: middle; |
| + | width: 0; |
| + | height: 0; |
| + | border-left: 6px solid transparent; |
| + | border-right: 6px solid transparent; |
| + | border-top: 6px solid black; |
| + | } |
| + | |
| + | label { |
| + | cursor: pointer; |
| + | } |
| + | |
| + | #HZAUmenu-toggle { |
| + | display: none; |
| + | } |
| + | |
| + | #HZAUmenu { |
| + | display: none; |
| + | font-size: 17.5px; |
| + | padding: 0 50px 0 50px; |
| + | width: 800px; |
| + | margin: 0; |
| + | color: black; |
| + | } |
| + | |
| + | #HZAUmenu-toggle:checked+#HZAUmenu { |
| + | display: block; |
| } | | } |
| </style> | | </style> |
| </head> | | </head> |
| | | |
− | <body> | + | <body data-spy="scroll" data-target="#myScrollspy" data-offset="20"> |
− | <div class="nav_body">
| + | <div class="container"> |
− | <a href="https://2017.igem.org/Team:HZAU-China" class="HZAU-logo" title="logo">
| + | <div class="row"> |
− | <img src="https://static.igem.org/mediawiki/2017/b/be/HZAU_2017_logo_1.png" height="100%">
| + | <nav class="col-sm-3" id="myScrollspy"> |
− | </a> | + | <div class="container-fluid"> |
− | <ul id="nav_organ">
| + | <div class="container-fluid"> |
− | <li class="Home"> | + | <ul class="nav nav-pills nav-stacked"> |
− | <a class="nav_tissue" href="https://2017.igem.org/Team:HZAU-China">Home</a>
| + | <li class="active" id="change_top"> |
− | </li>
| + | <a href="#section1">Section 1</a> |
− | <li class="Team">
| + | </li> |
− | <a class="nav_tissue" href="https://2017.igem.org/Team:HZAU-China/Team">Team</a> | + | <li> |
− | </li>
| + | <a href="#section2">Section 2</a> |
− | <li class="Project menu_item">
| + | </li> |
− | <a class="nav_tissue" href="">Project
| + | <li> |
− | <span class="caret"></span> | + | <a href="#section3">Section 3</a> |
− | </a>
| + | </li> |
− | <ul class="nav_cell shadow">
| + | </ul> |
− | <li>
| + | </div> |
− | <a href="https://2017.igem.org/Team:HZAU-China/Project/Description">Description</a>
| + | </div> |
− | </li>
| + | </nav> |
− | <li>
| + | <div class="col-sm-9"> |
− | <a href="https://2017.igem.org/Team:HZAU-China/Project/Design">Design</a>
| + | <div> |
− | </li>
| + | <div id="section1"> |
− | <li>
| + | </div> |
− | <a href="https://2017.igem.org/Team:HZAU-China/Project/Experiments">Experiments</a>
| + | </div> |
− | </li>
| + | <a class="biaoti">Description</a> |
− | <li>
| + | <a class="zhengwen">The replication of prokaryotes can be divided into three phases, B C and D, while there are multi-rounds of replication |
− | <a href="https://2017.igem.org/Team:HZAU-China/Project/Notebook">Notebook</a>
| + | happened in one cell. So both the phase and the copy number of genome is various in a culture.</a> |
− | </li>
| + | <div> |
− | <li>
| + | <label for="HZAUmenu-toggle" class="zhengwen">To know more about replication |
− | <a href="https://2017.igem.org/Team:HZAU-China/Project/InterLab">InterLab</a>
| + | <span class="caret_black"></span> |
− | </li>
| + | </label> |
− | <li>
| + | <input type="checkbox" id="HZAUmenu-toggle" /> |
− | <a href="https://2017.igem.org/Team:HZAU-China/Project/Model">Model</a>
| + | <ul id="HZAUmenu"> |
− | </li>
| + | <a>The replication process of E.coli can be divided into three phases, phase B, phase C and phase D. B is also called |
− | <li>
| + | pre-replication phase in which cells are preparing for DNA replication, like G1 phase of eukaryotes. C is also |
− | <a href="https://2017.igem.org/Team:HZAU-China/Project/Results">Results</a>
| + | called replication phase in which the genome is under replication corresponding to phase S in eukaryotic cell |
− | </li>
| + | cycle. The last D phase, of course, is called post-replication phase, in which chromosome separates and one |
− | <li>
| + | cell divides into two corresponding to G2 and M phase in eukaryotes. Among the three phases, C and D are relatively |
− | <a href="https://2017.igem.org/Team:HZAU-China/Project/Demonstrate">Demonstrate</a> | + | constant, about 40 min and 20 min separately, so when to initiate a replication determines the whole cell cycle. |
− | </li> | + | Recent research reveals a relationship between replication initiation and cell volume, but many details are |
− | <li>
| + | still remain unknown. But what we know is that a protein, DnaA, plays an important role in this process. |
− | <a href="https://2017.igem.org/Team:HZAU-China/Project/Improve">Improve</a>
| + | <br> DnaA is a versatile protein possessing many different functions related to cell cycle, among which the most |
− | </li>
| + | important one is to attached to the OriC, the origins of chromosome replication, and initiates replication. |
− | <li>
| + | So controlling cell cycle by interrupting the attachment of DnaA and corresponding DNA sequence with dCas9 |
− | <a href="https://2017.igem.org/Team:HZAU-China/Project/Attributions">Attributions</a>
| + | is an efficient approach. |
− | </li>
| + | <br> The whole cell cycle of eukaryotes and prokaryotes show a certain similarity, but there is difference between |
− | </ul> | + | them. The cell cycle of prokaryotes can overlap, which means the next round of replication initiates before |
− | </li> | + | the last replication complete, while eukaryotic cell cycle initiates one after another. Experiments have shown |
− | <li class="Parts menu_item"> | + | that even an isogenetic bacteria grown in the same culture show a difference in both replication phase and |
− | <a class="nav_tissue" href="">Parts
| + | genome copy numbers and this becomes a huge noise when constructing the 3D genome structure and building up |
− | <span class="caret"></span>
| + | a circuit related to genome gene. Besides, the development of synthetic biology requires a system to control |
− | </a>
| + | the reproduction of engineered organisms. So we believe that our project would be a useful tool no matter in |
− | <ul class="nav_cell shadow">
| + | theoretical areas or application areas. |
− | <li>
| + | </a> |
− | <a href="https://2017.igem.org/Team:HZAU-China/Parts/Parts">Parts</a>
| + | </ul> |
− | </li>
| + | </div> |
− | <li>
| + | <img src="https://static.igem.org/mediawiki/2017/4/44/T--HZAU-China--BCDperiod.png" class="tu_1"> |
− | <a href="https://2017.igem.org/Team:HZAU-China/Parts/Basic Parts">Basic Parts</a>
| + | <div> |
− | </li>
| + | <div id="section2"> |
− | <li> | + | </div> |
− | <a href="https://2017.igem.org/Team:HZAU-China/Parts/Composite Parts">Composite Parts</a>
| + | </div> |
− | </li> | + | <a class="zhengwen">Our problem arises from a research about constructing 4D genome of eukaryote. So we begin to wonder why there isn’t |
− | <li>
| + | a 4D genome project of prokaryote. After research we find that due to its complicated replication mechanism, there |
− | <a href="https://2017.igem.org/Team:HZAU-China/Parts/Part Collection">Part Collection</a>
| + | will be a huge noise while detecting its structure, which hinder the research on prokaryotic genome. |
− | </li>
| + | <br>Besides, the heterogenicity of cells are gathering importance recently in different fields.</a> |
− | </ul> | + | <img src="https://static.igem.org/mediawiki/2017/a/a6/T--HZAU-China--computorHand.png" class="tu_2"> |
− | </li>
| + | <div> |
− | <li class="Safety">
| + | <div id="section3"> |
− | <a class="nav_tissue" href="">Safety</a> | + | </div> |
− | </li>
| + | </div> |
− | <li class="HP menu_item">
| + | <a class="zhengwen">Therefore we begin to think if there could be a methods to eliminate the heterogeneity. While thinking deeper into |
− | <a class="nav_tissue" href="">Human Practices | + | this problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a |
− | <span class="caret"></span>
| + | new phenomenon that can change the traditional statements? |
− | </a>
| + | <br>So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same |
− | <ul class="nav_cell shadow">
| + | time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more |
− | <li>
| + | accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our |
− | <a href="https://2017.igem.org/Team:HZAU-China/Human Practices/Silver HP">Silver HP</a>
| + | synchronization system can also be controlled by machine, by program.</a> |
− | </li> | + | </div> |
− | <li>
| + | |
− | <a href="https://2017.igem.org/Team:HZAU-China/Human Practices/Integrated and Gold">Integrated and Gold</a>
| + | |
− | </li>
| + | |
− | <li>
| + | |
− | <a href="https://2017.igem.org/Team:HZAU-China/Human Practices/Public Engagement">Public Engagement</a>
| + | |
− | </li>
| + | |
− | </ul> | + | |
− | </li>
| + | |
− | <li class="Awards menu_item">
| + | |
− | <a class="nav_tissue" href="">Awards
| + | |
− | <span class="caret"></span>
| + | |
− | </a>
| + | |
− | <ul class="nav_cell shadow">
| + | |
− | <li> | + | |
− | <a href="https://2017.igem.org/Team:HZAU-China/Awards/Hardware">Hardware</a>
| + | |
− | </li> | + | |
− | </ul>
| + | |
− | </li>
| + | |
− | <li class="Judging Form">
| + | |
− | <a class="nav_tissue" href="https://2017.igem.org/Team:HZAU-China/Judging Form">Judging Form</a>
| + | |
− | </li>
| + | |
− | </ul>
| + | |
− | </div>
| + | |
− | <div class="HZAU_2017_menu">
| + | |
− | <img src="https://static.igem.org/mediawiki/2017/8/8e/HZAU_2017_background_1.png" class="beijin">
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− | <ul class="daohang">
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− | <li id="HZAU_menu_1">
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− | <a href="#" id="bianse_1">Background</a>
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− | </li>
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− | <li id="HZAU_menu_2">
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− | <a href="#item2_1" id="bianse_2">Information</a>
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− | </li>
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− | <li id="HZAU_menu_3">
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− | <a href="#item3_1" id="bianse_3">Why light control</a>
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− | </li>
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− | </ul>
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− | </div>
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− | <div class="HZAU_content wrap">
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− | <div class="item" id="item1">
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− | <a class="biaoti">Description</a>
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− | <a class="zhengwen">In the long history of life evolution, different species living in different environment developed the most adaptive
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− | surviving mechanism for them under the selection pressure, among which some unmatchable become widly preserved in
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− | different creatures, and the mechanism of replication is one of them. The majority of prokaryote share the same mechanism
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− | of self-replication including DNA replication, chromosome seperation and cell division. These three parts comprised
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− | the whole cell cycle, and the main model creature for researching the replication process is E.coli, which at the
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− | same time is the main object of our project.</a>
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− | </div>
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− | <img src="https://static.igem.org/mediawiki/2017/b/bc/HZAU_2017_tu1_1.png" class="tu_1">
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− | <div id="item2_1" style="padding:29px"></div>
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− | <div class="item" id="item2">
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− | <a class="zhengwen">The replication process of E.coli replication can be devided into phase B,C and D, three phases. Phase B, also called
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− | pre-replication phase, is for DNA replication proparation similar as G1 phase in eukaryote cell cycle. Phase C is
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− | regarded as replication phase during which the chromosome is replicating corespond to the S phase in eukaryote. The
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− | last phase, phase D, is called post-replication phase, in which the behave of chromosome seperation and cell division
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− | happend just like what happened in G2 and M phase of eukaryote cells. the time of C phase and D phase in a generation
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− | is relative constant, so what actually determines the cell cycle is the initiation of genome replication. We know
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− | that cell have to garantee that each cell should have at least one chormosome, and this requires a highly coordination
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− | between DNA replication and cell divsion. Though the delicate mechanism of the corrdination is still a puzzle, it
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− | is clear that DnaA protein plays a significant role in this event. DnaA is a versatile protein, it can not only behave
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− | like a helicase opening the duble-helix of replication initiation site, OriC, while have the function of raising
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− | other proteins forming into the replisome to prolong the replication, but also can anchor the replicationg choromosome
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− | on the cell membrane leading the following seperation of chromosome. But what makes it the linker between replication
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− | and division is that its concentration determines the initiation volume of replication. So according to these information,
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− | we think that regulating the combination of DNA and DnaA protein will be an efficient method to regulate cell replication.
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− | <a href="">For more information please click here.</a>
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− | </a>
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− | </div>
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− | <img src="https://static.igem.org/mediawiki/2017/5/51/HZAU_2017_tu2_1.png" class="tu_2">
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− | <div id="item3_1" style="padding:29px"></div>
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− | <div class="item" id="item3">
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− | <a class="zhengwen">There will be about 1h for an entire genome replication, but cell division can reach about 20min per generation. this
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− | reflects the multi-copy number of bacterial genome and only with this mechanism can E.coli reproduce in such a high
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− | speed. what's worse is that even isogenic cells grow in the same culture have a scattering distribution not only
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− | in phase but also in genome copy number. the strong heterogenic in cell cycle and genome copy number hinds the 3D
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− | genome research and may be a potential noise on genome gene expression. At the same time, the developing synthetic
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− | biology requires an approach that can control cell reproduction and can be easily incorporated into circuits, so
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− | that the whole system can be more safe and controlable. taking these into consider, we decided to use CRISPRi to
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− | inhibit its cell cycle, for more details please see design.</a>
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− | </div>
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− | <a class="ppp"></a>
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− | <div id="item4_1" style="padding:29px"></div>
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− | <div class="item" id="item4">
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− | <a class="zhengwen">But barely pause the replication in some circumstance can't satisfy our needs, and long time inhibition will prolong
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− | its length, so there is a need to totally control its replication, not only block but also open. with that purpose
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− | we decided to use optogentic to control this system, and the operablity of light to computer makes our system programable,
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− | which can be called 4C.</a>
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