Team:HZAU-China/Project/Description
Description
The replication of prokaryotes can be divided into three phases, B C and D, while there are multi-rounds of replication
happened in one cell. So both the phase and the copy number of genome is various in a culture.
To know more about replication
The replication process of E.coli can be divided into three phases, phase B, phase C and phase D. B is also called
pre-replication phase in which cells are preparing for DNA replication, like G1 phase of eukaryotes. C is also called
replication phase in which the genome is under replication corresponding to phase S in eukaryotic cell cycle. The
last D phase, of course, is called post-replication phase, in which chromosome separates and one cell divides into
two corresponding to G2 and M phase in eukaryotes. Among the three phases, C and D are relatively constant, about
40 min and 20 min separately, so when to initiate a replication determines the whole cell cycle. Recent research
reveals a relationship between replication initiation and cell volume, but many details are still remain unknown.
But what we know is that a protein, DnaA, plays an important role in this process.
DnaA is a versatile protein possessing many different functions related to cell cycle, among which the most important one is to attached to the OriC, the origins of chromosome replication, and initiates replication. So controlling cell cycle by interrupting the attachment of DnaA and corresponding DNA sequence with dCas9 is an efficient approach.
The whole cell cycle of eukaryotes and prokaryotes show a certain similarity, but there is difference between them. The cell cycle of prokaryotes can overlap, which means the next round of replication initiates before the last replication complete, while eukaryotic cell cycle initiates one after another. Experiments have shown that even an isogenetic bacteria grown in the same culture show a difference in both replication phase and genome copy numbers and this becomes a huge noise when constructing the 3D genome structure and building up a circuit related to genome gene. Besides, the development of synthetic biology requires a system to control the reproduction of engineered organisms. So we believe that our project would be a useful tool no matter in theoretical areas or application areas.
Our problem arises from a research about constructing 4D genome of eukaryote. So we begin to wonder why there isn’t
a 4D genome project of prokaryote. After research we find that due to its complicated replication mechanism, there
will be a huge noise while detecting its structure, which hinder the research on prokaryotic genome.
Besides, the heterogenicity of cells are gathering importance recently in different fields.
Therefore we begin to think if there could be a methods to eliminate the heterogeneity. While thinking deeper into
this problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new
phenomenon that can change the traditional statements?
So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program.
DnaA is a versatile protein possessing many different functions related to cell cycle, among which the most important one is to attached to the OriC, the origins of chromosome replication, and initiates replication. So controlling cell cycle by interrupting the attachment of DnaA and corresponding DNA sequence with dCas9 is an efficient approach.
The whole cell cycle of eukaryotes and prokaryotes show a certain similarity, but there is difference between them. The cell cycle of prokaryotes can overlap, which means the next round of replication initiates before the last replication complete, while eukaryotic cell cycle initiates one after another. Experiments have shown that even an isogenetic bacteria grown in the same culture show a difference in both replication phase and genome copy numbers and this becomes a huge noise when constructing the 3D genome structure and building up a circuit related to genome gene. Besides, the development of synthetic biology requires a system to control the reproduction of engineered organisms. So we believe that our project would be a useful tool no matter in theoretical areas or application areas.
Our problem arises from a research about constructing 4D genome of eukaryote. So we begin to wonder why there isn’t
a 4D genome project of prokaryote. After research we find that due to its complicated replication mechanism, there
will be a huge noise while detecting its structure, which hinder the research on prokaryotic genome.
Besides, the heterogenicity of cells are gathering importance recently in different fields.
Therefore we begin to think if there could be a methods to eliminate the heterogeneity. While thinking deeper into
this problem, it becomes interesting that what would happen if all the cells are synchronized, will there be a new
phenomenon that can change the traditional statements?
So in our mind the ideal synchronization methods should be not only simply inhibit the cell cycle but at the same time can free the inhibition according to our decision. As we all know, the manipulation of machine is much more accurate than hum beings, and there is a trend to let machine help us to control the organisms, so we want our synchronization system can also be controlled by machine, by program.
