Results
In this project, we achieved that control cell in the aspect of cell replication, proved that the feasibility of using CRISPR to regulate cell cycle(Figure 1.), and further regulate it with light(Figure 2.). A reverse regulation of our system is demonstrated by OD measurement and qPCR (Figure 3.). At the same time, a corresponding software and hardware are developed to achieve the computer controlled cell cycle. The software is used to predict the internal replication procession and control the hardware to achieve reall-time regulation. For more information please view software and hardware