Team:HokkaidoU Japan/Notebook

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Notebook

 

8/21

 

Transformation

Researcher: Kono, Esashi, Sugishita, Shimada

Part: BBa_R0010 on pSB1C3

 

8/22

 

Liquid Culture

Researcher: Kishimoto, Kono, Esashi, Sugishita, Shimada

Part: BBa_R0010 on pSB1C3

 

8/23

 

Mini-Prep

Researcher: Kishimoto, Kono, Esashi, Sugishita, Shimada

Part: BBa_R0010 on pSB1C3

 

Electrophoresis

Researcher: Kishimoto, Kono, Esashi, Sugishita, Shimada

Part: BBa_R0010 on pSB1C3

 

8/24

 

Liquid Culture

Researcher: Kono, Sugishita, Shimada

Part: BBa_R0010 on pSB1C3

 

8/25

 

Mini-Prep

Researcher: Hirao, Kono, Kuroiwa, Nakamura, Shimada

Part: BBa_R0010 on pSB1C3

 

8/30

 

NanoDrop

Researcher: Kishimoto, Kono, Sugishita, Sato

Part: BBa_R0010 on pSB1C3

 

Digestion

Researcher: Kishimoto, Kono, Sugishita, Sato

Part: BBa_R0010 on pSB1C3

 

Electrophoresis

Researcher: Kishimoto, Kono, Sugishita, Sato, Shimada, Takagi

Part: BBa_R0010, pSB1C3

 

8/31

 

Digestion

Researcher: Hirao, Kishimoto, Kono, Sugishita, Sato

Part: BBa_R0010 on pSB1C3

 

Electrophoresis

Researcher: Hirao, Kishimoto, Kono, Sugishita, Sato

Part: BBa_R0010, pSB1C3

 

Liquid Culture

Researcher: Hirao, Kono, Shimada

Part: BBa_R0010 on pSB1C3

 

9/1

 

Mini-Prep

Researcher: Hirao, Hirao, Sugishita, Sato, Shimada

Part: BBa_R0010 on pSB1C3

 

Electrophoresis

Researcher: Hirao, Kono, Sugishita, Sato, Shimada

Part: BBa_R0010 on pSB1C31

 

NanoDrop

Researcher:

Part: BBa_R0010 on pSB1C31

 

Digestion

Researcher: Hirao, Kono, Sugishita, Sato, Shimada

Part: BBa_R0010 on pSB1C3

 

Electrophoresis

Researcher: Hirao, Kono, Sugishita, Sato, Shimada

Part: BBa_R0010, pSB1C3

 

Electrophoresis

Researcher:

Part: BBa_R0010, pSB1C3

 

Gel Extraction

Researcher:

Part: BBa_R0010, pSB1C3

 

Electrophoresis

Researcher:

Part: BBa_R0010, pSB1C3

 

9/6

 

Transformation

Researcher: Hirao, Esashi, Noda, Sugishita, Shimada

Part: BBa_J23100 on J61002, BBa_J23102 on J61002, BBa_J23104 on J61002, BBa_K912000 on pSB1C3, BBa_K912001 on pSB1C3

 

9/7

 

Liquid Culture

Researcher: Esashi

Part: BBa_K912001 on pSB1C3

 

Transformation

Researcher: Hirao, Esashi

Part: BBa_J23100 on J61002, BBa_J23102 on J61002, BBa_J23104 on J61002

 

9/8

 

Mini-Prep

Researcher: Hirao, Kono, Esashi, Sugishita, Kawata

Part: BBa_K912001 on pSB1C3

 

Electrophoresis

Researcher: Hirao, Kono, Esashi, Sugishita

Part: BBa_K912001 on pSB1C3

 

NanoDrop

Researcher: Hirao

Part: BBa_K912001 on pSB1C3

 

Digestion

Researcher: Hirao

Part: BBa_K912001 on pSB1C3, pUC19

 

Electrophoresis

Researcher: Hirao

Part: BBa_K912001, pSB1C3, pUC19

 

9/11

 

Electrophoresis

Researcher: Hirao, Esashi

Part: BBa_K912001, pSB1C3, pUC19

 

Transformation

Researcher: Hirao, Esashi, Sugishita

Part: pUC19

 

Electrophoresis

Researcher: Hirao, Esashi, Sugishita

Part: BBa_K912001, pSB1C3, pUC19

 

Ethanol Precipitation

Researcher: Hirao, Esashi, Sugishita, Sato

Part: BBa_K912001

 

Gel Extraction

Researcher: Hirao, Esashi, Sugishita, Sato

Part: BBa_K912001

 

Electrophoresis

Researcher: Hirao, Esashi, Sugishita, Sato

Part: BBa_K912001

 

Electrophoresis

Researcher: Hirao, Esashi, Sugishita, Sato

Part: BBa_K912001

 

Liquid Culture

Researcher:

Part: BBa_J23100 on J61002, BBa_J23102 on J61002, BBa_J23104 on J61002

 

9/12

 

NanoDrop

Researcher: Hirao, Sato

Part: BBa_K912001, pSB1C3

 

Mini-Prep

Researcher: Hirao, Esashi, Sugishita

Part: BBa_J23100 on J61002, BBa_J23102 on J61002, BBa_J23104 on J61002

 

Electrophoresis

Researcher: Hirao, Esashi, Sugishita

Part: BBa_J23100 on J61002, BBa_J23102 on J61002, BBa_J23104 on J61002

 

9/13

 

Digestion

Researcher: Hirao, Saito

Part: BBa_K912001 on pSB1C3

 

Electrophoresis

Researcher: Hirao, Saito

Part: BBa_K912001

 

Ethanol Precipitation

Researcher: Hirao, Noda, Saito

Part: BBa_K912001

 

Electrophoresis

Researcher: Hirao, Noda, Sito

Part: BBa_K912001

 

Gel Extraction

Researcher: Hirao, Noda, Saito

Part: BBa_K912001

 

9/14

 

NanoDrop

Researcher: Hirao, Noda, Saito

Part: BBa_K912001

 

Electrophoresis

Researcher: Hirao, Kishimoto, Saito

Part: BBa_K912001

 

Electrophoresis

Researcher: Hirao, Kishimoto, Saito

Part: BBa_K912001

 

Mini-Prep

Researcher: Noda

Part: pUC19

 

Electrophoresis

Researcher: Hirao, Noda, Saito

Part: pUC19

 

NanoDrop

Researcher: Hirao

Part: pUC19

 

9/15

 

Digestion

Researcher: Hirao, Saito

Part: BBa_K912001 on pSB1C3

 

Electrophoresis

Researcher: Hirao, Saito, Sugishita, Shimada

Part: BBa_K912001, pSB1C3

 

Ethanol Precipitation

Researcher: Sugishita

Part: BBa_K912001 on pSB1C3

 

Gel Extraction

Researcher: Hirao, Shimada

Part: BBa_K912001

 

Electrophoresis

Researcher:

Part: BBa_K912001

 

9/19

 

Transformation

Researcher:

Part: BBa_K912000 on pSB1C3

 

Liquid Culture

Researcher:

Part: BBa_K912001 on pSB1C3

 

9/20

 

Mini-Prep

Researcher: Kono

Part: BBa_K912001 on pSB1C3

 

Electrophoresis

Researcher: Kono

Part: BBa_K912001 on pSB1C3

 

Electrophoresis

Researcher: Hirao

Part: BBa_K912001 on pSB1C3

 

PCR

Researcher:

Part: BBa_K912001 on pSB1C3

 

Electrophoresis

Researcher:

Part: BBa_K912001 on pSB1C3

 

9/20

 

Liquid Culture

Researcher: Hirao

Part: BBa_K912000 on pSB1C3

 

NanoDrop

Researcher: Kono

Part: BBa_K912001 on pSB1C3

 

9/21

 

Mini-Prep

Researcher: Kono

Part: BBa_K912000 on pSB1C3

 

Electrophoresis

Researcher: Kono, Sugishita

Part: BBa_K912000 on pSB1C3

 

NanoDrop

Researcher: Sugishita

Part: BBa_K912000 on pSB1C3

 

PCR

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

 

Electrophoresis

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

 

PCR Clean-up

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

 

NanoDrop

Researcher: Sugishita

Part: INSERT 5

 

Digestion

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

 

Digestion

Researcher: Kono, Esashi, Sugishita

Part: BBa_K912001 on pSB1C3

 

9/22

 

Electrophoresis

Researcher: Kono, Esashi

Part: INSERT 5

 

9/23

 

PCR

Researcher: Kono, Esashi

Part: INSERT 5

 

PCR Clean-up

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

 

NanoDrop

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

 

Digestion

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

 

NanoDrop

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

 

Electrophoresis

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

 

Ethanol Precipitation

Researcher: Kono, Esashi, Sugishita, Sato

Part: INSERT 5

 

Gel Extraction

Researcher: Hirao, Kono, Esashi, Sugishita, Sato

Part: INSERT 5

 

NanoDrop

Researcher: Hirao, Kono, Esashi, Sugishita, Sato

Part: INSERT 5

 

Ligation

Researcher: Hirao, Kono, Esashi, Sugishita, Sato

Part: pSB1C3

 

Electrophoresis

Researcher: Hirao, Esashi

Part: pSB1C3

 

Ligation

Researcher: Kono

Part: pSB1C3

 

Electrophoresis

Researcher: Kono

Part: pSB1C3

 

Ligation

Researcher: Kono

Part: BBa_K912001, INSERT 5

 

9/24

 

PCR

Researcher: Hirao

Part: INSERT 5

 

PCR Clean-up

Researcher: Hirao

Part: INSERT 5

 

NanoDrop

Researcher: Hirao

Part: INSERT 5

 

Digestion

Researcher: Hirao

Part: INSERT 5

 

Digestion

Researcher: Hirao

Part: BBa_K912001

 

Ethanol Precipitation

Researcher: Hirao

Part: BBa_K912001

 

Gel Extraction

Researcher: Hirao, Nishimura

Part: BBa_K912001, INSERT 5

 

Transformation

Researcher: Hirao, Nishimura

Part: BBa_K912001 _ INSERT 5

 

Gel Extraction

Researcher: Hirao, Nishimura

Part: BBa_K912001, INSERT 5

 

NanoDrop

Researcher: Hirao

Part: BBa_K912001, INSERT 5

 

Ligation

Researcher: Hirao

Part: BBa_K912001, INSERT 5

 

Ethanol Precipitation

Researcher: Kono

Part: BBa_K912001 _ INSERT 5

 

Transformation

Researcher: Kono

Part: BBa_K912001 _ INSERT 5

 

9/25

 

Ligation

Researcher: Kamo, Sugishita

Part: BBa_K912001, INSERT 5

 

PCR

Researcher: Esashi, Saito

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Ligation

Researcher: Kamo, Sugishita

Part: BBa_K912001, INSERT 5

 

PCR Clean-up

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Electrophoresis

Researcher: Esashi, Kamo, Saito, Sugishita

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Digestion

Researcher: Kono, Sugishita

Part: pSB1C3, INSERT 1

 

9/26

 

Transformation

Researcher: Kono

Part: pSB1C3 - INSERT 5

 

Ethanol Precipitation

Researcher: Hirao

Part: pSB1C3, INSERT 1

 

NanoDrop

Researcher: Kono, Kamo, Saito

Part: pSB1C3, INSERT 1

 

Digestion

Researcher: Kono, Kamo, Saito

Part: pSB1C3, INSERT 1

 

Digestion

Researcher: Kono, Saito

Part: BBa_K912001, INSERT 5

 

Ethanol Precipitation

Researcher: Kono

Part: BBa_K912001, INSERT 1

 

Ethanol Precipitation

Researcher: Saito

Part: BBa_K912001, INSERT 5

 

Electrophoresis

Researcher: Saito

Part: BBa_K912001, INSERT 1

 

PCR

Researcher: Hirao

Part: INSERT 5

 

Ethanol Precipitation

Researcher: Hirao, Kishimoto

Part: BBa_K921000, BBa_K912001

 

PCR

Researcher: Hirao, Esashi

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Ethanol Precipitation

Researcher: Esashi

Part: BBa_K921000, BBa_K912001

 

Digestion

Researcher: Hirao, Esashi

Part: BBa_K921000, BBa_K912001

 

PCR Clean-up

Researcher: Hirao, Esashi

Part: INSERT 5

 

NanoDrop

Researcher: Esashi

Part: INSERT 5

 

Ethanol Precipitation

Researcher: Hirao

Part: BBa_K921000, BBa_K912001

 

PCR Clean-up

Researcher: Esashi

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

PCR Clean-up

Researcher: Hirao

Part: INSERT 5

 

NanoDrop

Researcher: Hirao

Part: INSERT 5

 

NanoDrop

Researcher: Esashi

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

9/27

 

NanoDrop

Researcher: Hirao

Part: BBa_K912001

 

Digestion

Researcher: Hirao, Esashi

Part: INSER T 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

Liquid Culture

Researcher: Hirao

Part: BBa_K912000, BBa_K912001

 

Gel Extract

Researcher: Hirao

Part: BBa_K912000, BBa_K912001

 

NanoDrop

Researcher: Hirao

Part: BBa_K912000, BBa_K912001

 

Electrophoresis

Researcher: Hirao

Part: INSER T 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

Ethanol Precipitation

Researcher: Hirao

Part: INSER T 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

Gel Extract

Researcher: Hirao

Part: INSER T 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

PCR

Researcher: Kono, Saito

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

Mini-Prep

Researcher: Kono, Saito

Part: BBa_K912000, BBa_K912001

 

PCR Clean-up

Researcher: Saito

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

Electrophoresis

Researcher: Kono, Saito

Part: BBa_K912000, BBa_K912001

 

PCR

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

PCR

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

9/28

 

PCR Clean-up

Researcher: Hirao, Kono

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

NanoDrop

Researcher: Kono

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

Ethanol Precipitation

Researcher: Kono

Part: BBa_K912000, BBa_K912001

 

NanoDrop

Researcher: Kono

Part: BBa_K912000, BBa_K912001

 

PCR

Researcher: Kono

Part: pSB1C3

 

PCR Clean-up

Researcher: Kono

Part: pSB1C3

 

Gel Extract

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

NanoDrop

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

NanoDrop

Researcher: Kono

Part: pSB1C3

 

Electrophoresis

Researcher: Kono

Part: pSB1C3

 

PCR

Researcher: Esashi

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

PCR

Researcher: Esashi, Saito

Part: pSB1C3

 

Digestion

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

9/29

 

Electrophoresis

Researcher: Hirao

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

PCR Clean-up

Researcher: Hirao

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

Gel Extraction

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

NanoDrop

Researcher: Hirao

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

NanoDrop

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

Digestion

Researcher: Kono, Saito

Part: BBa_K912000, BBa_K912001

 

Mini-Prep

Researcher: Noda

Part: BBa_K912000, BBa_K912001

 

Ethanol Precipitation

Researcher: Kono, Esashi, Saito

Part: BBa_K912000, BBa_K912001

 

NanoDrop

Researcher: Kono, Esashi, Saito

Part: BBa_K912000, BBa_K912001

 

Digestion

Researcher: Esashi, Saito

Part: BBa_K912000, BBa_K912001

 

Digestion

Researcher: Esashi, Saito

Part: BBa_K912000, BBa_K912001

 

Gel Extraction

Researcher: Esashi, Saito

Part: BBa_K912000, BBa_K912001

 

NanoDrop

Researcher: Kono, Esashi

Part: BBa_K912000, BBa_K912001

 

9/30

 

Ligation

Researcher: Kono

Part: BBa_K912000 _ INSERT 5, BBa_K912001 _ INSERT 5

 

Digestion

Researcher: Hirao, Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

Digestion

Researcher: Kono

Part: BBa_K912000, BBa_K912001

 

PCR

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

Ethanol Precipitation

Researcher: Kono

Part: BBa_K912000, BBa_K912001, INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

Gel Extract

Researcher: Kono

Part: BBa_K912000, BBa_K912001, INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

NanoDrop

Researcher: Kono

Part: BBa_K912000, BBa_K912001, INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

Ethanol Precipitation

Researcher: Hirao

Part: BBa_K912000 - INSERT 5, BBa_K912001 _ INSERT 5

 

PCR Clean-up

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

NanoDrop

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

PCR

Researcher: Hirao

Part: INSERT 2, INSERT 3, INSERRT 4

 

PCR Clean-up

Researcher: Hirao, Kamo

Part: INSERT 2, INSERT 3, INSERRT 4

 

Electrophoresis

Researcher: Hirao, Kamo

Part: INSERT 2, INSERT 3, INSERRT 4

 

NanoDrop

Researcher: Hirao, Kamo

Part: INSERT 2, INSERT 3, INSERRT 4

 

Mini-Prep

Researcher: Hirao

Part: BBa_K912000, BBa_K912001

 

NanoDrop

Researcher: Hirao

Part: BBa_K912000, BBa_K912001

 

Ethanol Precipitation

Researcher: Kono

Part: BBa_K912000, BBa_K912001

 

Digestion

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

Mini-Prep

Researcher: Kono

Part: BBa_K912000, BBa_K912001

 

NanoDrop

Researcher: Kono

Part: BBa_K912000, BBa_K912001

 

Ethanol Precipitation

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

Colony PCR

Researcher: Kono

Part: BBa_K912000

 

Gel Extraction

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

NanoDrop

Researcher: Kono

Part: INSERT 1, INSERT 4

 

10/1

 

PCR

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

PCR Clean-up

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

NanoDrop

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Digestion

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Mini-Prep

Researcher: Kono

Part: BBa_K912000 on pSB1C3, BBa_K912001 on pSB1C3

 

NanoDrop

Researcher: Kono

Part: BBa_K912000 on pSB1C3, BBa_K912001 on pSB1C3

 

10/2

 

Digestion

Researcher: Hirao, Kono

Part: BBa_K912000 on pSB1C3, BBa_K912001 on pSB1C3

 

Ethanol Precipitation

Researcher: Hirao, Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Gel Extraction

Researcher: Hirao, Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

NanoDrop

Researcher: Hirao, Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Gel Extraction

Researcher: Hirao, Kono

Part: BBa_K912000 on pSB1C3, BBa_K912001 on pSB1C3

 

NanoDrop

Researcher: Hirao, Kono

Part: BBa_K912000, BBa_K912001

 

Ligation

Researcher: Hirao, Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, BBa_K912000

 

Ligation

Researcher: Hirao

Part: BBa_K912001, INSERT 5

 

10/3

 

Colony PCR

Researcher: Kono

Part: BBa_K912000

 

Transformation

Researcher: Kono, Sugishita

Part: INSERT 1 _ INSERT 2 on pSB1C3, INSERT 1 _ INSERT 3 on pSB1C3, INSERT 1 _ INSERT 4 on pSB1C3, INSERT 5 on K912001

 

PCR

Researcher: Hirao

Part: BBa_K912001, INSERT 5

 

10/4

 

Electrophoresis

Researcher: Kishimoto, Kono

Part: BBa_K912001, INSERT 5

 

Colony PCR

Researcher: Kishimoto, Kono

Part: INSERT 1 _ INSERT 2 on pSB1C3, INSERT 1 _ INSERT 3 on pSB1C3, INSERT 1 _ INSERT 4 on pSB1C3

 

Liquid Culture

Researcher: Hirao

Part: INSERT 1 _ INSERT 2 on pSB1C3, INSERT 1 _ INSERT 3 on pSB1C3, INSERT 1 _ INSERT 4 on pSB1C3, BBa_K912001 _ INSERT 5 on pSB1C3

 

Mini-Prep

Researcher: Kamo, Sugishita

Part: INSERT 1 _ INSERT 2 on pSB1C3, INSERT 1 _ INSERT 3 on pSB1C3, INSERT 1 _ INSERT 4 on pSB1C3, BBa_K912001 _ INSERT 5 on pSB1C3

 

Electrophoresis

Researcher: Sugishita

Part: INSERT 1 _ INSERT 2 on pSB1C3, INSERT 1 _ INSERT 3 on pSB1C3, INSERT 1 _ INSERT 4 on pSB1C3, BBa_K912001 _ INSERT 5 on pSB1C3

 

10/6

 

Digestion

Researcher: Kono

Part: INSERT 1 _ INSERT 2 on pSB1C3, INSERT 1 _ INSERT 3 on pSB1C3, INSERT 1 _ INSERT 4 on pSB1C3, BBa_K912001 _ INSERT 5 on pSB1C3

 

Electrophoresis

Researcher: Kono

Part: INSERT 1 _ INSERT 2, INSERT 1 _ INSERT 3, INSERT 1 _ INSERT 4, BBa_K912001 _ INSERT 5

 

Electrophoresis

Researcher: Kono

Part: INSERT 1 _ INSERT 2, INSERT 1 _ INSERT 3, INSERT 1 _ INSERT 4, BBa_K912001 _ INSERT 5

 

Ligation

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

10/7

 

Electrophoresis

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, INSERT 5

 

Digestion

Researcher: Kono

Part: BBa_K912000 on pSB1C3, BBa_K912001 on pSB1C3

 

PCR

Researcher: Noda

Part: INSERT 1, INSERT 2, INSERT 3

 

Ethanol Precipitation

Researcher: Kono

Part: BBa_K912000, BBa_K912001

 

Electrophoresis

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3

 

Ethanol Precipitation

Researcher: Kono

Part: BBa_K912000 on pSB1C3

 

NanoDrop

Researcher: Kono

Part: BBa_K912000 on pSB1C3

 

PCR Clean-up

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3

 

Gel Extraction

Researcher: Kono

Part: BBa_K912000

 

Electrophoresis

Researcher: Kono

Part: BBa_K912000

 

10/8

 

Gel Extraction

Researcher: Hirao

Part: BBa_K912000, BBa_K912001

 

PCR

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3

 

Electrophoresis

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, BBa_K912000, BBa_K912001

 

NanoDrop

Researcher: Hirao

Part: BBa_K912000, BBa_K912001

 

Ligation

Researcher: Kono

Part: BBa_K912000, BBa_K912001

 

PCR Clean-up

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3

 

NanoDrop

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3

 

10/9

 

Electrophoresis

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3

 

Gel Extraction

Researcher: Hirao, Kono

Part: INSERT 5

 

NanoDrop

Researcher: Hirao

Part: INSERT 5

 

Liquid Culture

Researcher: Hirao

Part: BBa_K912000 on pSB1C3

 

Ligation

Researcher: Kono

Part: INSERT 5

 

Ligation

Researcher: Kono

Part: INSERT 5, BBa_K912001

 

Ethanol Precipitation

Researcher:

Part: INSERT 5, BBa_K912001

 

Electrophoresis

Researcher: Hirao, Kamo

Part: INSERT 5

 

Mini-Prep

Researcher: Hirao

Part: BBa_K912000 on pSB1C3

 

NanoDrop

Researcher: Hirao

Part: BBa_K912000 on pSB1C3

 

Transformation

Researcher: Kono

Part: BBa_K912001 _ INSERT 5

 

10/10

 

Liquid Culture

Researcher: Hirao

Part: BBa_R0010 on pSB1C3, BBa_K592009 on pSB1C3

 

PCR

Researcher: Kono

Part: INSERT 3, INSERT 4, INSERT 5

 

Electrophoresis

Researcher: Kono

Part: INSERT 3, INSERT 4, INSERT 5

 

Colony PCR

Researcher: Kono

Part: BBa_K912001 - INSERT 5

 

Digestion

Researcher: Kono

Part: BBa_K912000 on pSB1C3, pUC19

 

Electrophoresis

Researcher: Kono

Part: BBa_K912000 on pSB1C3, pUC19

 

Mini-Prep

Researcher: Kono

Part: BBa_R0010 on pSB1C3, BBa_K592009 on pSB1C3

 

NanoDrop

Researcher: Kono

Part: BBa_R0010 on pSB1C3, BBa_K592009 on pSB1C3

 

PCR

Researcher: Kono

Part: INSERT 3, INSERT 4

 

Electrophoresis

Researcher: Kono

Part: INSERT 3, INSERT 4, BBa_K912001 - INSERT

 

Digestion

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, BBa_R0010, BBa_K592009

 

PCR Clean-up

Researcher: Hirao

Part: INSERT 3, INSERT 4, INSERT 5

 

NanoDrop

Researcher: Hirao

Part: INSERT 3, INSERT 4, INSERT 5

 

Digestion

Researcher: Kono

Part: INSERT 4, INSERT 5

 

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, BBa_R0010, BBa_K592009

 

10/11

 

Gel Extraction

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, BBa_R0010, BBa_K592009

 

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 4, INSERT 5

 

Gel Extraction

Researcher: Hirao, Kono

Part: INSERT 4, INSERT 5

 

NanoDrop

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, BBa_R0010, BBa_K592009

 

NanoDrop

Researcher: Kono

Part: INSERT 4, INSERT 5

 

Ligation

Researcher: Kono

Part: INSERT 1 _ INSERT 2. INSERT 1 _ INSERT 3, INSERT 1 _ INSERT 4

 

Ligation

Researcher: Kono

Part: BBa_R0010

 

10/11

 

PCR

Researcher: Kono

Part: INSERT 1, INSERT 3

 

Ethanol Precipitation

Researcher: Kono

Part: INSERT 1 _ INSERT 2. INSERT 1 _ INSERT 3, INSERT 1 _ INSERT 4

 

Electrophoresis

Researcher: Kono

Part: BBa_R0010

 

Transformation

Researcher: Kono

Part: INSERT 1 _ INSERT 2. INSERT 1 _ INSERT 3, INSERT 1 _ INSERT 4

 

Electrophoresis

Researcher: Kono

Part: INSERT 1, INSERT 3

 

Mini-Prep

Researcher: Hirao

Part: BBa_K912001 _ INSERT 5 on pSB1C3, BBa_R0010 on pSB1C3, BBa_K912000 on pSB1C3

 

NanoDrop

Researcher: Hirao

Part: BBa_K912001 _ INSERT 5 on pSB1C3, BBa_R0010 on pSB1C3, BBa_K912000 on pSB1C3

 

Colony PCR

Researcher: Hirao, Kono

Part: BBa_K912001 _ INSERT 5 on pSB1C3

 

Electrophoresis

Researcher: Hirao

Part: BBa_K912001 _ INSERT 5 on pSB1C3

 

Digestion

Researcher: Hirao, Kono

Part: BBa_R0010 on pSB1C3, BBa_K912000 on pSB1C3

 

Ethanol Precipitation

Researcher: Hirao

Part: BBa_R0010 on pSB1C3, BBa_K912000 on pSB1C3

 

10/12

 

Gel Extraction

Researcher: Hirao

Part: BBa_R0010, BBa_K912000

 

NanoDrop

Researcher: Hirao

Part: BBa_R0010, BBa_K912000

 

Electrophoresis

Researcher: Hirao

Part: BBa_K921001 _ Part 5

 

Colony PCR

Researcher: Kono

Part: INSERT 1 _ INSERT 2. INSERT 1 _ INSERT 3, INSERT 1 _ INSERT 4

 

10/13

 

Digestion

Researcher: Kono

Part: BBa_K921000 on pSB1C3, BBa_K912001 on pSB1C3

 

Ethanol Precipitation

Researcher: Hirao

Part: BBa_K921000, BBa_K912001

 

Gel Extraction

Researcher: Hirao

Part: BBa_K921000, BBa_K912001

 

NanoDrop

Researcher: Hirao

Part: BBa_K921000, BBa_K912001

 

Electrophoresis

Researcher: Hirao

Part: BBa_K921000, BBa_K912001

 

Digestion

Researcher: Kono

Part: INSERT 1, INSERT 3, BBa_K921000 on pSB1C3, BBa_K912001

 

Ligation

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 4

 

Electrophoresis

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 4

 

Ethanol Precipitation

Researcher: Kono

Part: INSERT 1, INSERT 3, BBa_K912000, BBa_K912001

 

Gel Extract

Researcher: Hirao

Part: INSERT 1, INSERT 3, BBa_K912000, BBa_K912001

 

10/14

 

NanoDrop

Researcher: Hirao

Part: INSERT 1, BBa_K912000, BBa_K912001

 

PCR

Researcher: Kono

Part: INSERT 3, INSERT 5

 

Ligation

Researcher: Kono

Part: INSERT 1 _ INSERT 2, INSERT 1 _ INSERT 4

 

Ligation

Researcher: Kono

Part: BBa_K912000 _ INSERT 5, BBa_K912001 _ INSERT 5

 

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 1 _ INSERT 2 _ pSB1C3, INSERT 1 _ INSERT 4 _ pSB1C3, BBa_R0010, BBa_K912000 _ INSERT 5, BBa_K912000, BBa_K912001 _ INSERT 5, BBa_K912001

 

Electrophoresis

Researcher: Hirao

Part: INSERT 3, INSERT 5

 

PCR Clean-up

Researcher: Hirao

Part: INSERT 3, INSERT 5

 

NanoDrop

Researcher: Hirao

Part: INSERT 3, INSERT 5

 

Digestion

Researcher: Hirao

Part: INSERT 3, INSERT 5

 

Transformation

Researcher: Hirao

Part: INSERT 1 _ INSERT 2 on pSB1C3, INSERT 1 _ INSERT 4 on pSB1C3, BBa_R0010

 

10/15

 

Ethanol Precipitation

Researcher: Kono

Part: INSERT 3, INSERT 5

 

Electrophoresis

Researcher: Kono

Part: INSERT 3, INSERT 5

 

Gel Extraction

Researcher: Kono

Part: INSERT 3, INSERT 5

 

NanoDrop

Researcher: Kono

Part: INSERT 3, INSERT 5

 

Transformation

Researcher: Kono

Part: BBa_K912000 _ INSERT 5 on pSB1C3, BBa_K912991 _ INSERT 5 on pSB1C3

 

Ligation

Researcher: Kono

Part: INSERT 1 _ INSERT 3 on pSB1C3

 

Colony PCR

Researcher: Kono

Part: INSERT 1- INSERT 2, INSERT 1 _ INSERT 4

 

Digestion

Researcher: Kono

Part: BBa_K912000 on pSB1C3, BBa_K912001 on pSB1C3

 

Colony PCR

Researcher: Kono

Part: INSERT 1 _ INSERT 2, INSERT 1 _ INSERT 4

 

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 1 _ INSERT 3 on pSB1C3

 

Colony PCR

Researcher: Hirao

Part: INSERT 1 _ INSERT 2 on pSB1C3, INSERT 1 _ INSERT 4 on pSB1C3

 

Transformation

Researcher: Hirao

Part: INSERT 1 _ ISNERT 3 on pSB1C3

 

Ethanol Precipitation

Researcher: Kono

Part: BBa_K912000, BBa_K912001

 

Electrophoresis

Researcher: Kono

Part: INSERT 1 _ INSERT 2 on pSB1C3, INSERT 1 _ INSERT 4 on pSB1C3

 

10/16

 

Gel Extraction

Researcher: Kono

Part: BBa_K912000, BBa_K912001

 

NanoDrop

Researcher: Kono

Part: BBa_K912000, BBa_K912001

 

Ligation

Researcher: Kono

Part: BBa_K912000 _ INSERT 5 on pSB1C3, BBa_K912001 _ INSERT 5 on pSB1C3, BBa_R0010 on pSB1C3

 

Colony PCR

Researcher: Kono

Part: INSERT 1 _ INSERT 2 on pSB1C3, INSERT 1 _ INSERT 4 on pSB1C3

 

Electrophoresis

Researcher: Kono

Part: INSERT 1 _ INSERT 2 on pSB1C3, INSERT 1 _ INSERT 4 on pSB1C3

 

Ethanol Precipitation

Researcher: Kono

Part: BBa_K912000 _ INSERT 5 on pSB1C3, BBa_K912001 _ INSERT 5 on pSB1C3, BBa_R0010 on pSB1C3

 

Colony PCR

Researcher: Kono

Part: INSERT 1 _ INSERT 3 on pSB1C3, BBa_K912000 _ INSERT 5 on pSB1C3, BBa_K912001 _ INSERT 5 on pSB1C3

 

Electrophoresis

Researcher: Kono

Part: INSERT 1 _ INSERT 3 on pSB1C3, BBa_K912000 _ INSERT 5 on pSB1C3, INSERT 5

 

Digestion

Researcher: Kono

Part: BBa_K592009 on pSB1C3

 

Liquid Culture

Researcher: Kono

Part: BBa_K912000 _ INSERT 5, BBa_K592009 on pSB1C3, BBa_K912001 on pSB1C3

 

10/17

 

Ethanol Precipitation

Researcher: Kono

Part: BBa_K592009

 

Mini-Prep

Researcher: Kono

Part: BBa_K912000 _ INSERT 5 on pSB1C3, BBa_K592009 on pSB1C3, BBa_K912001 on pSB1C3

 

NanoDrop

Researcher: Kono

Part: BBa_K912000 _ INSERT 5 on pSB1C3, BBa_K592009 on pSB1C3, BBa_K912001 on pSB1C3

 

Electrophoresis

Researcher: Kono

Part: BBa_K912000 _ INSERT 5 on pSB1C3, BBa_K592009 on pSB1C3, BBa_K912001 on pSB1C3

 

Digestion

Researcher: Kono

Part: BBa_K912000 _ INSERT 5 on pSB1C3, BBa_K912001 _ INSERT 5 on pSB1C3

 

Electrophoresis

Researcher: Kishimoto

Part: BBa_K912000 _ INSERT 5, BBa_K912001 _ INSERT 5

 

Ethanol Precipitation

Researcher: Hirao

Part: BBa_K912000 _ INSERT 5, BBa_K912001 _ INSERT 5

 

10/18

 

PCR

Researcher: Hirao

Part: BBa_K912000 _ INSERT 5, BBa_K912001 _ INSERT 5

 

Gel Extraction

Researcher: Hirao

Part: BBa_K912000 _ INSERT 5

 

NanoDrop

Researcher: Hirao

Part: BBa_K912000 _ INSERT 5

 

Ligation

Researcher: Hirao

Part: BBa_K912000 _ INSERT 5

 

Electrophoresis

Researcher: Hirao

Part: BBa_K912000 _ INSERT 5

 

Ligation

Researcher: Kono

Part: BBa_K912000 _ INSERT 5

 

Ligation

Researcher: Kono

Part: INSERT 2, INSERT 3, INSERT 4

 

PCR

Researcher: Kono

Part: BBa_K912000 _ INSERT 5, BBa_912001 _ INSERT 5

 

Electrophoresis

Researcher: Kono

Part: BBa_K912000 _ INSERT 5, BBa_912001 _ INSERT 5

 

Ligation

Researcher: Kono

Part: INSERT 2 _ pSB1C3, INSERT 3 _ pSB1C3, INSERT 4 _ pSB1C3

 

10/19

 

Ethanol Precipitation

Researcher: Kono

Part: INSERT 2 _ pSB1C3, INSERT 3 _ pSB1C3, INSERT 4 _ pSB1C3

 

Gel Extraction

Researcher: Kono

Part: BBa_K912000 _ INSERT 5, BBa_K912001 _ INSERT 5

 

Transformation

Researcher: Kono, Kamo

Part: INSERT 2 _ pSB1C3, INSERT 3 _ pSB1C3, INSERT 4 _ pSB1C3

 

PCR

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

10/20

 

PCR Clean-up

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

NanoDrop

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Digestion

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Colony PCR

Researcher: Hirao, Kono

Part: INSERT 2 on pSB1C3, INSERST 4 on pSB1C3

 

Ethanol Precipitation

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Electrophoresis

Researcher: Kono

Part: INSERT 2 on pSB1C3, INSERST 4 on pSB1C3

 

10/21

 

Transformation

Researcher: Kono

Part: INSERT 2 on pSB1C3, INSERST 4 on pSB1C3

 

Liquid Culture

Researcher: Kono

Part: INSERT 2 on pSB1C3

 

Gel Extraction

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

NanoDrop

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Ligation

Researcher: Kono

Part: INSERT 2 on pSB1C3, INSERT 3 on pSB1C3

 

Mini-Prep

Researcher: Kono

Part: INSERT 4 on pSB1C3

 

NanoDrop

Researcher: Kono

Part: INSERT 4 on pSB1C3

 

Ethanol Precipitation

Researcher: Kono

Part: INSERT 2 on pSB1C3, INSERT 3 on pSB1C3

 

Colony PCR

Researcher: Hirao

Part: INSERT 2 on pSB1C3

 

Transformation

Researcher: Hirao, Kono

Part: INSERT 2 on pSB1C3, INSERT 4 on pSB1C3

 

Electrophoresis

Researcher: Hirao, Kono

Part: INSERT 4 on pSB1C3

 

Digestion

Researcher: Hirao

Part: INSERT 4 on pSB1C3

 

10/22

 

Electrophoresis

Researcher: Hirao

Part: INSERT 2 on pSB1C3, INSERT 4 on pSB1C3

 

Liquid Culture

Researcher: Kono

Part: INSERT 2 on pSB1C3

 

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 4 on pSB1C3

 

Electrophoresis

Researcher: Hirao

Part: INSERT 2 on pSB1C3, INSERT 3 on pSB1C3, INSERT 4

 

Gel Extraction

Researcher: Hirao

Part: INSERT 4 on pSB1C3

 

NanoDrop

Researcher: Hirao

Part: INSERT 4 on pSB1C3

 

Ligation

Researcher: Hirao

Part: INSERT 4 on pSB1C3

 

Electrophoresis

Researcher: Hirao

Part: INSERT 4 on pSB1C3

 

Colony PCR

Researcher: Hirao

Part: INSERT 2 on pSB1C3, INSERT 3 on pSB1C3

 

Mini-Prep

Researcher: Hirao

Part: INSERT 2 on pSB1C3

 

NanoDrop

Researcher: Hirao

Part: INSERT 2 on pSB1C3

 

Digestion

Researcher: Hirao

Part: INSERT 2 on pSB1C3

 

Electrophoresis

Researcher: Hirao

Part: INSERT 2 on pSB1C3, INSERT 2

 

10/23

 

PCR

Researcher: Hirao

Part: INSERT 4

 

Electrophoresis

Researcher: Hirao

Part: INSERT 2 on pSB1C3, INSERT 3 on pSB1C3

 

Colony PCR

Researcher: Hirao, Kono

Part: INSERT 2 on pSB1C3, INSERT 3 on pSB1C3

 

PCR

Researcher: Hirao, Kono

Part: INSERT 1, INSERT 2, INSERT 3

 

PCR Clean-up

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3

 

10/24

 

Ligation

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, pSB1C3

 

Electrophoresis

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, pSB1C3

 

PCR

Researcher: Kono

Part: INSERT 4

 

Digestion

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3

 

PCR Clean-up

Researcher: Hirao

Part: INSERT 4

 

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3

 

NanoDrop

Researcher: Hirao

Part: INSERT 4

 

Digestion

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, BBa_K912000 _ INSERT 5

 

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, BBa_K912000 _ INSERT 5

 

Gel Extraction

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, BBa_K912000 _ INSERT 5

 

Electrophoresis

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4, BBa_K912000 _ INSERT 5

 

Digestion

Researcher: Hirao

Part: BBa_K912000 _ INSERT 5

 

Mini-Prep

Researcher: Hirao

Part: BBa_K912000 _ INSERT 5

 

NanoDrop

Researcher: Hirao

Part: BBa_K912000 _ INSERT 5

 

Ethanol Precipitation

Researcher: Kono

Part: BBa_K912000 _ INSERT 5

 

10/25

 

Electrophoresis

Researcher: Kono

Part: BBa_K912000 _ INSERT 5

 

NanoDrop

Researcher: Kono

Part: BBa_K912000 _ INSERT 5

 

Ligation

Researcher: Kono

Part: INSERT 2 _ pSB1C3, INSERT 3 _ pSB1C3, INSERT 4 _ pSB1C3

 

Digestion

Researcher: Hirao

Part: BBa_K912000 _ INSERT 5

 

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 2 _ pSB1C3, INSERT 3 _ pSB1C3, INSERT 4 _ pSB1C3, pSB1C3

 

Transformation

Researcher: Hirao

Part: INSERT 2 _ pSB1C3, INSERT 3 _ pSB1C3, INSERT 4 _ pSB1C3, pSB1C3

 

PCR

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Digestion

Researcher: Hirao

Part: BBa_K912000 _ INSERT 5

 

PCR Clean-up

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

NanoDrop

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Mini-Prep

Researcher: Kono

Part: BBa_K912000, BBa_K912001

 

Digestion

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Digestion

Researcher: Kono

Part: pSB1C3

 

Electrophoresis

Researcher: Hirao

Part: pSB1C3

 

Ethanol Precipitation

Researcher: Hirao

Part: pSB1C3

 

Gel Extraction

Researcher: Hirao

Part: pSB1C3

 

Ethanol Precipitation

Researcher: Kono

Part: pSB1C3

 

Electrophoresis

Researcher: Hirao

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Electrophoresis

Researcher: Kono

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

10/26

 

Gel Extraction

Researcher: Kono

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

NanoDrop

Researcher: Hirao

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Gel Extraction

Researcher: Kono

Part: pSB1C3, INSERT 2, INSERT 3, INSERT 4

 

NanoDrop

Researcher: Kono

Part: pSB1C3, INSERT 2, INSERT 3, INSERT 4

 

Ligation

Researcher: Hirao

Part: INSERT 1 on pSB1C3, INSERT 2 on pSB1C3, INSERT 3 on pSB1C3, INSERT 4 on pSB1C3

 

Ligation

Researcher: Kono

Part: INSERT 1 _ INSERT 2 on pSB1C3, INSERT 1 _ INSERT 3 on pSB1C3, INSERT 1 _ INSERT 4 on pSB1C3

 

Ligation

Researcher: Kono

Part: INSERT 1 _ pSB1C3, INSERT 2 _ pSB1C3, INSERT 3 _ pSB1C3, INSERT 4 _ pSB1C3

 

Ethanol Precipitation

Researcher: Hirao, Kono

Part: INSERT 1 on pSB1C3, INSERT 2 on pSB1C3, INSERT 3 on pSB1C3, INSERT 4 on pSB1C3, INSERT 1 _ INSERT 2 on pSB1C3, INSERT 1 _ INSERT 3 on pSB1C3, INSERT 1 _ INSERT 4 on pSB1C3

 

Transformation

Researcher: Hirao, Kono

Part: INSERT 1 on pSB1C3, INSERT 2 on pSB1C3, INSERT 3 on pSB1C3, INSERT 4 on pSB1C3, INSERT 1 _ INSERT 2 on pSB1C3, INSERT 1 _ INSERT 3 on pSB1C3, INSERT 1 _ INSERT 4 on pSB1C3

 

Colony PCR

Researcher: Hirao, Kono

Part: INSERT 2 on pSB1C3, INSERT 3 on pSB1C3, INSERT 4 on pSB1C3

 

Electrophoresis

Researcher: Kono

Part: INSERT 2 on pSB1C3, INSERT 3 on pSB1C3, INSERT 4 on pSB1C3

 

10/27

 

Colony PCR

Researcher: Kono

Part: INSERT 1 _ INSERT 2 on pSB1C3, INSERT 1 _ INSERT 3 on pSB1C3, INSERT 1 _ INSERT 4 on pSB1C3

 

PCR

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT 4

 

Colony PCR

Researcher: Kono

Part: INSERT 1 _ INSERT 2 on pSB1C3, INSERT 1 _ INSERT 3 on pSB1C3, INSERT 1 _ INSERT 4 on pSB1C3

 

10/28

 

PCR

Researcher: Kono

Part: INSERT 1 on pSB1C3, INSERT 2 on pSB1C3, INSERT 3 on pSB1C3, INSERT 4 on pSB1C3

 

NanoDrop

Researcher: Kono

Part: INSERT 1 on pSB1C3, INSERT 2 on pSB1C3, INSERT 3 on pSB1C3, INSERT 4 on pSB1C3

 

10/29

 

Protein Extraction

Researcher: Hirao, Kono

Part: INSERT 2 on pSB1C3, INSERT 3 on pSB1C3

 

10/30

 

Protein Extraction

Researcher: Hirao, Kono

Part: INSERT 2 on pSB1C3, INSERT 3 on pSB1C3

 

10/31

 

Protein Extraction

Researcher: Hirao, Kono

Part: INSERT 2 on pSB1C3, INSERT 3 on pSB1C3

 

11/1

 

Protein Concentration

Researcher: Hirao, Kono

Part: INSERT 2 on pSB1C3, INSERT 3 on pSB1C3

 

Activity Measurement

Researcher: Hirao, Kono

Part: INSERT 2 on pSB1C3, INSERT 3 on pSB1C3

 

SDS-PAGE

Researcher: Hirao, Kishimoto

Part: INSERT 2 on pSB1C3, INSERT 3 on pSB1C3