Team:NEFU China/Protocols

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Protocol

On this page, the NEFU team would like to share with you the protocols that we have been using over the summer. This library of protocols has been developed alongside our supervisors for the purpose of this study. Here you can find the exact methods we use to generate our data and results. We share them in the interest of reproducibility.

Cultural Conditions

Vector Construction

Expression of The Recombinant Protein CALB

Extraction of Bacterial Membrane

Sodium Alginate Ball Embedding

Clear Zone

Chemotaxis Protocol

Detecting of Leucine

Fatty Acid Producing Detecting

Fatty Acid Detection of Leaders

Fatty Acid Binding Protein Detecting

Fatty Acid Absorbing Detecting