Difference between revisions of "Team:HK SKHLPSS"
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<img class="img-responsive" src="http://placehold.it/2000x300/d3d3d3/f2f2f2"> | <img class="img-responsive" src="http://placehold.it/2000x300/d3d3d3/f2f2f2"> | ||
| − | <h2> Welcome to iGEM 2017! </h2> | + | |
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| + | <h2> Welcome to iGEM 2017! </h2> | ||
| + | <p>Your team has been approved and you are ready to start the iGEM season! </p> | ||
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</div> | </div> | ||
| − | + | <h2>Project Description</h2> | |
| + | <p> | ||
| + | A Self-Assembled DNA Nanocube for the Diagnosis of H3N2 Influenza | ||
| + | </p> | ||
| + | <p> | ||
| + | DNA is always used a genetic material for information transfer over generations. Its programmability also allows the application in fabricating various objects for diagnostics and therapeutics as the field of DNA nanotechnology. This year, we used DNA to fabricate a three-dimensional cube that is responsive to the presence of H3N2 influenza mRNA biomarker. We used DNA and RNA oligos of the same sequence of the target for detection. From the result, we found that the DNA nanocube specifically responses to the presence of target leading to the opening of the lid. This results in the disassemble of the quadruplex formation and reduction in hemin-mediated peroxidase activity. We proved that a three-dimensional nanodevice can be used for quick diagnosis within 30 minutes and it is applicable for the detection of different biomarkers and we wish to largely produce this diagnostic device with engineered bacteria. | ||
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| + | The biodiversity of organism is originated from the information carried on DNA that it is only constructed by the fundamental Watson-Crick base pairing rules governing two basic combination of base pairs. The programmability of DNA allows the use of it as LEGO bricks to build different objects in both two-dimensional and three-dimensional manner. | ||
| − | + | In addition to showing the possibility of programming DNA into different shapes, the field of DNA nanotechnology was developed to use it for medical purposes. Under the presence of target nucleic acids, conformational change of a molecular beacon that is also consisted of DNA will occur through a simple strand displacement process. Apart from nucleic acids target, presence of metal ions is also detectable by DNA molecular beacon as it affects the stability of DNA. To detect metal ion, G-quadruplex as a four-stranded nucleic acid structure is well known that the formation of it is highly depending on cation. The formation of quadruplex is able to stabilize the molecular structure of hemin and enhance its’ peroxidase activity that leads to the result of quantitative fluorescence or colorimetric signal. | |
| − | + | This year, the ultimate goal of our iGEM team is trying to establish a metal ion responsive molecular beacon in E. coli as a general platform for detecting environmental contamination using bacteria. In order to achieve this progressively, we will first perform the experiment in vitro using molecular beacon that consists of short oligos as a prove of concept. Then we will try to express the system in E. coli and conduct the same experiment using laboratory prepared sample and on-site collected sample for the detection of contamination. | |
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| − | + | <hr / > | |
| − | + | <div class="row"> | |
| − | + | <div class="col-md-6"> | |
| − | + | <h3>Before you start: </h3> | |
| − | + | <p> Please read the following pages:</p> | |
| − | + | <ul> | |
| − | + | <li> <a href="https://2017.igem.org/Competition">Competition Hub</a> </li> | |
| − | + | <li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li> | |
| − | + | <li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li> | |
| − | + | </ul> | |
| − | + | </div> | |
| − | + | <div class="col-md-6"> | |
| − | + | <div class="highlight"> | |
| − | + | <h3> Styling your wiki </h3> | |
| − | + | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your | |
| − | + | project. | |
| − | + | </p> | |
| − | + | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style | |
| − | + | beyond what has been provided.</p> | |
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| − | + | ||
| − | + | ||
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| + | </div> | ||
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<a href="https://2017.igem.org/Special:Upload"> | <a href="https://2017.igem.org/Special:Upload"> | ||
| − | + | UPLOAD FILES | |
| − | + | </a> | |
</p> | </p> | ||
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Revision as of 06:40, 4 October 2017
Project Description
A Self-Assembled DNA Nanocube for the Diagnosis of H3N2 Influenza
DNA is always used a genetic material for information transfer over generations. Its programmability also allows the application in fabricating various objects for diagnostics and therapeutics as the field of DNA nanotechnology. This year, we used DNA to fabricate a three-dimensional cube that is responsive to the presence of H3N2 influenza mRNA biomarker. We used DNA and RNA oligos of the same sequence of the target for detection. From the result, we found that the DNA nanocube specifically responses to the presence of target leading to the opening of the lid. This results in the disassemble of the quadruplex formation and reduction in hemin-mediated peroxidase activity. We proved that a three-dimensional nanodevice can be used for quick diagnosis within 30 minutes and it is applicable for the detection of different biomarkers and we wish to largely produce this diagnostic device with engineered bacteria.
Before you start:
Please read the following pages:
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
