Difference between revisions of "Team:Grenoble-Alpes/LabBook"
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<div style="background:#27ae60; margin:0%; padding-bottom: 1%; margin-bottom: 2%;"><h2 class="titre_categorie" id="prep" > Step 1 : Target Preparation </h2></div> | <div style="background:#27ae60; margin:0%; padding-bottom: 1%; margin-bottom: 2%;"><h2 class="titre_categorie" id="prep" > Step 1 : Target Preparation </h2></div> | ||
| − | + | <h5>The first rate-limiting step in the detection of Vibrio Cholerae is the extraction of its DNA. To this aim, the bacteria has been deeply studied, as well as the current DNA extraction techniques.</h5> | |
<h2 style="padding-top:1%; padding-bottom:1%; color:#27ae60; font-size:3vw;"> 1. Vibrio Cholerae </h2> | <h2 style="padding-top:1%; padding-bottom:1%; color:#27ae60; font-size:3vw;"> 1. Vibrio Cholerae </h2> | ||
| − | + | <h2 style="padding-top:1%; padding-bottom:1%; color:#27ae60; font-size:2vw;"> 1.1 Classification & Generalities </h2> | |
| + | <h5> V.Cholerae is a thin gram-negative proteobacterium that has a flagellum which gives it mobility [2]. This bacteria is responsible of cholera disease, causing severe contagious epidemia. This bacteria use to grow in basic conditions (Optimal growth pH : 9) [1] with 1-3% NaCl in liquid or solid mediums [3]. </h5> | ||
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| + | <h2 style="padding-top:1%; padding-bottom:1%; color:#27ae60; font-size:2vw;"> 1.2 Growth in laboratory </h2> | ||
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| + | <h5> Aeroanerobic bacteria grown on conventional media. Optimum growth in medium with 1 to 3% NaCl pH 9, in liquid media (Colonies in 3-4 h at the surface) or in solid media (colonies in 8-10 hours). The bacterium can also grow on bile salt media. According to these characteristics, alkaline peptone water pH 8.6 3% NaCl can be used as an enrichment medium as well as an alkaline agar pH 9. Colonies are 2 to 3 mm in diameter and are smooth, Flat and transparent. [3] </h5> | ||
Revision as of 20:10, 16 October 2017
LabBook
Facing back to the Etendard glacier, in between the peaks of Maurienne and the ones of Oisan. Credits: Estelle Vincent
Step 1 : Target Preparation
The first rate-limiting step in the detection of Vibrio Cholerae is the extraction of its DNA. To this aim, the bacteria has been deeply studied, as well as the current DNA extraction techniques.
1. Vibrio Cholerae
1.1 Classification & Generalities
V.Cholerae is a thin gram-negative proteobacterium that has a flagellum which gives it mobility [2]. This bacteria is responsible of cholera disease, causing severe contagious epidemia. This bacteria use to grow in basic conditions (Optimal growth pH : 9) [1] with 1-3% NaCl in liquid or solid mediums [3].
1.2 Growth in laboratory
Aeroanerobic bacteria grown on conventional media. Optimum growth in medium with 1 to 3% NaCl pH 9, in liquid media (Colonies in 3-4 h at the surface) or in solid media (colonies in 8-10 hours). The bacterium can also grow on bile salt media. According to these characteristics, alkaline peptone water pH 8.6 3% NaCl can be used as an enrichment medium as well as an alkaline agar pH 9. Colonies are 2 to 3 mm in diameter and are smooth, Flat and transparent. [3]
Step 2 :
Step 3 :
